The effect of the ethanolic extract of Laportea decumana  (Roxb.) Wedd. on the inflammatory, proliferative and  maturation stages of wound healing of an acute injury  using a rat model

Yulia Yusrini Djabir; Gemini Alam

Makale detayları

The effect of the ethanolic extract of Laportea decumana (Roxb.) Wedd. on the inflammatory, proliferative and maturation stages of wound healing of an acute injury using a rat model

Yazarlar:

Yulia Yusrini Djabir | Gemini Alam

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.81-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339595

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Laportea decumana Roxb. Wedd. is a plant that is traditionally used for its analgesic, antipyretic, antioxidant, anti-inflammatory, and antibacterial purposes. This study aimed to determine the wound-healing effects of fractionated L. decumana ethanol extract ointment on the inflammatory, proliferation, and maturation phases in a rat model of acute injury. L. decumana leaves were extracted with 70 ethanol and then fractionated with n-hexane with a centrifuge. The polar fraction was used in the animal model. Acute injury was induced in four areas on male rats n15, which were assigned to receive either Vaseline, 2 L. decumana extract, 4 L. decumana extract, or Myrhax ointment control. The wound histological assessments during the inflammatory, proliferation, and maturation phases were conducted on day 1, day 4, and day 9 after injury, respectively. The results show that the wound diameter on Day 9 was significantly lower with 4 L. decumana treatment than with Vaseline and 2 L. decumana treatment and was similar to the results of using Myrhax ointment. Histopathological examination showed that during the inflammatory phase, all wounds exhibited edema, leucocytes, and macrophages; however, during the proliferation phase, 4 L. decumana treatment resulted in significantly more granulation and fibroblasts, as well as thicker collagen and faster reepithelialization during the maturation phase compared to Vaseline-only treatment. In conclusion, 4 L. decumana demonstrated a potent wound-healing effect in the rat acute injury model, especially hastening the proliferation and maturation phases of wound healing.

 Laportea decumana Roxb. Wedd. is a plant that is traditionally used for its analgesic, antipyretic, antioxidant, anti-inflammatory, and antibacterial purposes. This study aimed to determine the wound-healing effects of fractionated L. decumana ethanol extract ointment on the inflammatory, proliferation, and maturation phases in a rat model of acute injury. L. decumana leaves were extracted with 70 ethanol and then fractionated with n-hexane with a centrifuge. The polar fraction was used in the animal model. Acute injury was induced in four areas on male rats n15, which were assigned to receive either Vaseline, 2 L. decumana extract, 4 L. decumana extract, or Myrhax ointment control. The wound histological assessments during the inflammatory, proliferation, and maturation phases were conducted on day 1, day 4, and day 9 after injury, respectively. The results show that the wound diameter on Day 9 was significantly lower with 4 L. decumana treatment than with Vaseline and 2 L. decumana treatment and was similar to the results of using Myrhax ointment. Histopathological examination showed that during the inflammatory phase, all wounds exhibited edema, leucocytes, and macrophages; however, during the proliferation phase, 4 L. decumana treatment resulted in significantly more granulation and fibroblasts, as well as thicker collagen and faster reepithelialization during the maturation phase compared to Vaseline-only treatment. In conclusion, 4 L. decumana demonstrated a potent wound-healing effect in the rat acute injury model, especially hastening the proliferation and maturation phases of wound healing.

 Laportea decumana Roxb. Wedd. is a plant that is traditionally used for its analgesic, antipyretic, antioxidant, anti-inflammatory, and antibacterial purposes. This study aimed to determine the wound-healing effects of fractionated L. decumana ethanol extract ointment on the inflammatory, proliferation, and maturation phases in a rat model of acute injury. L. decumana leaves were extracted with 70 ethanol and then fractionated with n-hexane with a centrifuge. The polar fraction was used in the animal model. Acute injury was induced in four areas on male rats n15, which were assigned to receive either Vaseline, 2 L. decumana extract, 4 L. decumana extract, or Myrhax ointment control. The wound histological assessments during the inflammatory, proliferation, and maturation phases were conducted on day 1, day 4, and day 9 after injury, respectively. The results show that the wound diameter on Day 9 was significantly lower with 4 L. decumana treatment than with Vaseline and 2 L. decumana treatment and was similar to the results of using Myrhax ointment. Histopathological examination showed that during the inflammatory phase, all wounds exhibited edema, leucocytes, and macrophages; however, during the proliferation phase, 4 L. decumana treatment resulted in significantly more granulation and fibroblasts, as well as thicker collagen and faster reepithelialization during the maturation phase compared to Vaseline-only treatment. In conclusion, 4 L. decumana demonstrated a potent wound-healing effect in the rat acute injury model, especially hastening the proliferation and maturation phases of wound healing.

 Laportea decumana Roxb. Wedd. is a plant that is traditionally used for its analgesic, antipyretic, antioxidant, anti-inflammatory, and antibacterial purposes. This study aimed to determine the wound-healing effects of fractionated L. decumana ethanol extract ointment on the inflammatory, proliferation, and maturation phases in a rat model of acute injury. L. decumana leaves were extracted with 70 ethanol and then fractionated with n-hexane with a centrifuge. The polar fraction was used in the animal model. Acute injury was induced in four areas on male rats n15, which were assigned to receive either Vaseline, 2 L. decumana extract, 4 L. decumana extract, or Myrhax ointment control. The wound histological assessments during the inflammatory, proliferation, and maturation phases were conducted on day 1, day 4, and day 9 after injury, respectively. The results show that the wound diameter on Day 9 was significantly lower with 4 L. decumana treatment than with Vaseline and 2 L. decumana treatment and was similar to the results of using Myrhax ointment. Histopathological examination showed that during the inflammatory phase, all wounds exhibited edema, leucocytes, and macrophages; however, during the proliferation phase, 4 L. decumana treatment resulted in significantly more granulation and fibroblasts, as well as thicker collagen and faster reepithelialization during the maturation phase compared to Vaseline-only treatment. In conclusion, 4 L. decumana demonstrated a potent wound-healing effect in the rat acute injury model, especially hastening the proliferation and maturation phases of wound healing.

Makale detayları

The effect of the ethanolic extract of Laportea decumana (Roxb.) Wedd. on the inflammatory, proliferative and maturation stages of wound healing of an acute injury using a rat model

Yazarlar:

Yulia Yusrini Djabir | Gemini Alam

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.81-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339595

pISSN:

2630-6344

Laportea decumana

wound healing

inflammation

proliferation

maturation

Sayı

Makale detayları

The effect of the ethanolic extract of Laportea decumana (Roxb.) Wedd. on the inflammatory, proliferative and maturation stages of wound healing of an acute injury using a rat model

Yazarlar: Yulia Yusrini Djabir | Gemini Alam

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy | Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.29 Issue 1, pp.81-90

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001339595

pISSN: 2630-6344

Laportea decumana

wound healing

inflammation

proliferation

maturation

Sayı

Yazarlar:

Yulia Yusrini Djabir | Gemini Alam

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.29 Issue 1, pp.81-90

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001339595

pISSN:

2630-6344