Somatic Embryogenesis from Immature Cotyledons of Apomictic and Non-Apomictic Seeds in Walnut (Juglans regia L.)

Bekir SAN; Hatice DUMANOGLU

Makale detayları

Somatic Embryogenesis from Immature Cotyledons of Apomictic and Non-Apomictic Seeds in Walnut (Juglans regia L.)

Yazarlar:

Bekir SAN | Hatice DUMANOGLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2006 Vol.30 Issue 2, pp.111-117

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000808906

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen of the apple cv. 'Golden Delicious' (Malus x domestica Borkh.). The best cotyledon stage for somatic embryogenesis was determined in open-pollinated seeds of 10 walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after anthesis. As a result of this experiment, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after anthesis. Driver and Kuniyuki walnut (DKW) medium supplemented with 1 mg l-1 6-benzylaminopurine (BAP), 2 mg l-1 kinetin, 0.01 mg l-1 indole-3-butyric acid (IBA) and 250 mg l-1 L-glutamine was used in initial cultures. Explants were transferred to DKW medium without growth regulators and L-glutamine in subcultures. The percentage of embryogenic cotyledons that originated from apomictic and non-apomictic seeds ranged from 3.6% to 25% and the number of embryos per cotyledon ranged from 1 to 9.7 at the end of the fourth subculture. A repetitively embryogenic embryo line originating from immature cotyledons of apomictic seeds of the Tokat-1 walnut genotype was maintained by secondary embryogenesis.

 

Somatic embryogenesis from immature cotyledons of apomictic and open-pollinated seeds in some walnut (Juglans regia L.) genotypes was investigated. To obtain apomictic seeds, female flowers were bagged and/or pollinated with pollen of the apple cv. 'Golden Delicious' (Malus x domestica Borkh.). The best cotyledon stage for somatic embryogenesis was determined in open-pollinated seeds of 10 walnut genotypes. Immature cotyledons were cultured 8, 9, 10, 11 and 12 weeks after anthesis. As a result of this experiment, cotyledons of seeds thought to be of apomictic origin were cultured 8 weeks after anthesis. Driver and Kuniyuki walnut (DKW) medium supplemented with 1 mg l-1 6-benzylaminopurine (BAP), 2 mg l-1 kinetin, 0.01 mg l-1 indole-3-butyric acid (IBA) and 250 mg l-1 L-glutamine was used in initial cultures. Explants were transferred to DKW medium without growth regulators and L-glutamine in subcultures. The percentage of embryogenic cotyledons that originated from apomictic and non-apomictic seeds ranged from 3.6% to 25% and the number of embryos per cotyledon ranged from 1 to 9.7 at the end of the fourth subculture. A repetitively embryogenic embryo line originating from immature cotyledons of apomictic seeds of the Tokat-1 walnut genotype was maintained by secondary embryogenesis.

Makale detayları

Somatic Embryogenesis from Immature Cotyledons of Apomictic and Non-Apomictic Seeds in Walnut (Juglans regia L.)

Yazarlar: Bekir SAN | Hatice DUMANOGLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2006 Vol.30 Issue 2, pp.111-117

Belge Tipi: Makale

Yayın Tarihi: 1/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000808906

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Walnut

Juglans regia L.

somatic embryogenesis

immature cotyledon

apomictic seed

Sayı

Yazarlar:

Bekir SAN | Hatice DUMANOGLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2006 Vol.30 Issue 2, pp.111-117

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000808906

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X