An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential

Cihan Bostancı; Hasan Nazır; Gönül Dönmez

Makale detayları

An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential

Yazarlar:

Cihan Bostancı | Hasan Nazır | Gönül Dönmez

Yayıncı:

Ankara University

Ana Başlıklar:

Structural Biology

Dergi:

Communications Faculty of Sciences University of Ankara Series C Biology Vol.32 Issue 1, pp.24-35

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283948

pISSN:

1303-6025

Anahtar kelimeler:

Özet:

 Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode SPE carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the PbII solution for electrochemical analysis. After the exposure time, cyclic voltammetry CV and differential pulse voltammetry DPV analyses were carried out. The study shows that the sensor was found in a linear range between 1x108 M and 8x108 M, with the lowest detection limit 109 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mVs, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.

 Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode SPE carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the PbII solution for electrochemical analysis. After the exposure time, cyclic voltammetry CV and differential pulse voltammetry DPV analyses were carried out. The study shows that the sensor was found in a linear range between 1x108 M and 8x108 M, with the lowest detection limit 109 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mVs, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.

 Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode SPE carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the PbII solution for electrochemical analysis. After the exposure time, cyclic voltammetry CV and differential pulse voltammetry DPV analyses were carried out. The study shows that the sensor was found in a linear range between 1x108 M and 8x108 M, with the lowest detection limit 109 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mVs, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.

 Biosensors are strong alternatives to conventional analytical techniques such as HPLC and spectroscopic techniques for water quality and heavy metal detection. Heavy metal contaminated waters can monitor by microbial biosensors efficiently. For this purpose, newly isolated Pseudomonas sp. is used to develop a highly sensitive low-cost microbial biosensor for water quality monitoring. The objective of the study is the invention of new high sensitive low-cost microbial biosensors to determine heavy metals in aqueous solutions and optimise the working conditions Pseudomonas marincola cells were embedded onto the Screen Printed Electrode SPE carbon surface and dried for 30 minutes at laminar flow cabinet. Developed microbial sensors were immersed into the PbII solution for electrochemical analysis. After the exposure time, cyclic voltammetry CV and differential pulse voltammetry DPV analyses were carried out. The study shows that the sensor was found in a linear range between 1x108 M and 8x108 M, with the lowest detection limit 109 M. The optimum pre-concentration time and scan rate were measured as 10 minutes and 10 mVs, respectively. The results support that the new isolated Pseudomonas sp. has significant potential to determine the trace amount of lead in aqueous solutions.

Makale detayları

An investigation of Pseudomonas marincola to determine Pb(II) biosensor potential

Yazarlar: Cihan Bostancı | Hasan Nazır | Gönül Dönmez

Yayıncı: Ankara University

Ana Başlıklar: Structural Biology

Dergi: Communications Faculty of Sciences University of Ankara Series C Biology Vol.32 Issue 1, pp.24-35

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283948

pISSN: 1303-6025

Pseudomonas marincola

biosensor

lead

heavy metal

Sayı

Yazarlar:

Cihan Bostancı | Hasan Nazır | Gönül Dönmez

Yayıncı:

Ankara University

Ana Başlıklar:

Structural Biology

Dergi:

Communications Faculty of Sciences University of Ankara Series C Biology Vol.32 Issue 1, pp.24-35

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283948

pISSN:

1303-6025