Effect of Rheum ribes Application on Immunohistochemical Localizations of TLR2 and TLR4 in the Liver and Kidneys of Diabetic Rats

Ayhan Akgün; Turgay Deprem

Makale detayları

Effect of Rheum ribes Application on Immunohistochemical Localizations of TLR2 and TLR4 in the Liver and Kidneys of Diabetic Rats

Yazarlar:

Ayhan Akgün | Turgay Deprem

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.21 Issue 3, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001332601

pISSN:

1304-7280

Anahtar kelimeler:

Özet:

 Diabetes mellitus DM causes various complications. The most important of these complications are those occurring in the liver and kidney, which are responsible for hemostasis. Rheum ribes R. ribes is used for treatment purposes in DM as well as various diseases. TLR family members are increased in many cases. However, the in-creased expression of TLR2 and TLR4 in diabetic patients is noteworthy. In our study, we aimed to examine the effects of R. ribes extract in diabetes as well as the immunohistochemical localization of TLR2 and TLR4, which are signifi-cantly increased in diabetic cases. Streptozotocin STZ was administered intraperitoneally 50 mgkg in DM subjects. R. ribes and diabetes  R. ribes groups were given 200 mgkg R. ribes extract by oral gavage for 21 days. In the exam-ination, TLR2 immunoreactivity in liver tissue was found in hepatocytes, central veins, and sinusoid endothelium; TLR4 immunoreactivity was seen in V. centralis, hepatocytes and V. interlobularis. In the kidney, TLR2 and TLR4 immunore-activity was observed in glomerulus cells, proximal tubules, distal tubules, and medulla. While TLR2 was cytoplasmic immunoreactive, TLR4 was both cytoplasmic and nuclear. Immunoreactivity for both TLR2 and TLR4 was observed intensely in the liver and kidney tissues of the diabetes group. But it was observed that immunoreactivity was signifi-cantly reduced in the liver and kidney tissues of the diabetic group administered R. ribes extract. As a result, it was determined that R. ribes plant has a positive effect on the liver and kidney tissues of diabetic rats. Therefore, we think that the R. ribes plant, which is also used in various diseases, will contribute to the development of new treatment methods in the pharmaceutical industry for DM disease.

 Diabetes mellitus DM causes various complications. The most important of these complications are those occurring in the liver and kidney, which are responsible for hemostasis. Rheum ribes R. ribes is used for treatment purposes in DM as well as various diseases. TLR family members are increased in many cases. However, the in-creased expression of TLR2 and TLR4 in diabetic patients is noteworthy. In our study, we aimed to examine the effects of R. ribes extract in diabetes as well as the immunohistochemical localization of TLR2 and TLR4, which are signifi-cantly increased in diabetic cases. Streptozotocin STZ was administered intraperitoneally 50 mgkg in DM subjects. R. ribes and diabetes  R. ribes groups were given 200 mgkg R. ribes extract by oral gavage for 21 days. In the exam-ination, TLR2 immunoreactivity in liver tissue was found in hepatocytes, central veins, and sinusoid endothelium; TLR4 immunoreactivity was seen in V. centralis, hepatocytes and V. interlobularis. In the kidney, TLR2 and TLR4 immunore-activity was observed in glomerulus cells, proximal tubules, distal tubules, and medulla. While TLR2 was cytoplasmic immunoreactive, TLR4 was both cytoplasmic and nuclear. Immunoreactivity for both TLR2 and TLR4 was observed intensely in the liver and kidney tissues of the diabetes group. But it was observed that immunoreactivity was signifi-cantly reduced in the liver and kidney tissues of the diabetic group administered R. ribes extract. As a result, it was determined that R. ribes plant has a positive effect on the liver and kidney tissues of diabetic rats. Therefore, we think that the R. ribes plant, which is also used in various diseases, will contribute to the development of new treatment methods in the pharmaceutical industry for DM disease.

 Diabetes mellitus DM causes various complications. The most important of these complications are those occurring in the liver and kidney, which are responsible for hemostasis. Rheum ribes R. ribes is used for treatment purposes in DM as well as various diseases. TLR family members are increased in many cases. However, the in-creased expression of TLR2 and TLR4 in diabetic patients is noteworthy. In our study, we aimed to examine the effects of R. ribes extract in diabetes as well as the immunohistochemical localization of TLR2 and TLR4, which are signifi-cantly increased in diabetic cases. Streptozotocin STZ was administered intraperitoneally 50 mgkg in DM subjects. R. ribes and diabetes  R. ribes groups were given 200 mgkg R. ribes extract by oral gavage for 21 days. In the exam-ination, TLR2 immunoreactivity in liver tissue was found in hepatocytes, central veins, and sinusoid endothelium; TLR4 immunoreactivity was seen in V. centralis, hepatocytes and V. interlobularis. In the kidney, TLR2 and TLR4 immunore-activity was observed in glomerulus cells, proximal tubules, distal tubules, and medulla. While TLR2 was cytoplasmic immunoreactive, TLR4 was both cytoplasmic and nuclear. Immunoreactivity for both TLR2 and TLR4 was observed intensely in the liver and kidney tissues of the diabetes group. But it was observed that immunoreactivity was signifi-cantly reduced in the liver and kidney tissues of the diabetic group administered R. ribes extract. As a result, it was determined that R. ribes plant has a positive effect on the liver and kidney tissues of diabetic rats. Therefore, we think that the R. ribes plant, which is also used in various diseases, will contribute to the development of new treatment methods in the pharmaceutical industry for DM disease.

 Diyabetes mellitus DM çeşitli komplikasyonlara sebep olmaktadır. Bu komplikasyonların en önemlileri hemostaz-dan sorumlu olan karaciğer ve böbrekte meydana gelen komplikasyonlardır. Işgın Rheum ribes, R. ribes çeşitli hasta-lıkların yanı sıra DMde de tedavi amaçlı kullanılmaktadır. TLR aile uyeleri birçok durumda artmaktadır. Ancak diyabetik hastalarda TLR2 ve TLR4teki ekspresyon artışı dikkat çekmektedir. Çalışmamızda R. ribes ekstraktının diyabetteki etkilerinin yanı sıra diyabetik olgularda belirgin olarak artan TLR2 ve TLR4un immunohistokimyasal lokalizasyonunu incelemeyi amaçladık. DM deneklerde Streptozotosin STZ 50 mgkg intraperitoneal uygulanarak oluşturuldu. R. ribes ve diyabet  R. ribes gruplarına 21 gun boyunca oral gavaj ile 200 mgkg R. ribes ekstraktı verildi. Yapılan incelemede karaciğer dokusunda TLR2 immunreaktivitesi hepatositlerde, V. sentralislerde, sinuzoid endotellerinde; TLR4 immunre-aktivitesi V. sentralis, hepatositler ve V. interlobularislerde göruldu. Böbrekte ise TLR2 ve TLR4 immunreaktivite glome-rulus hucrelerinde, proksimal tubullerde, distal tubullerde ve medullada gözlendi. TLR2 sitoplazmik immunreaktif oldu-ğu halde TLR4 hem sitoplazmik hem de nukleerdi. Hem TLR2 hem de TLR4 için immunreaktivite diyabet grubu karaci-ğer ve böbrek dokularında yoğun olarak gözlenirken, R. ribes ekstraktı uygulanan diyabet grubu karaciğer ve böbrek dokularında immunreaktivitenin önemli ölçude azaldığı gözlendi. Sonuç olarak diyabetik ratların karaciğer ve böbrek dokularında R. ribes bitkisinin pozitif bir etkiye sahip olduğu belirlenmiştir. Bundan dolayı çeşitli hastalıklarda da kullanı-lan R. ribes bitkisi DM hastalığı için ilaç endustrisinde yeni tedavi yöntemlerinin geliştirilmesine katkı sağlayacağını duşunmekteyiz.

Makale detayları

Effect of Rheum ribes Application on Immunohistochemical Localizations of TLR2 and TLR4 in the Liver and Kidneys of Diabetic Rats

Yazarlar:

Ayhan Akgün | Turgay Deprem

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.21 Issue 3, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001332601

pISSN:

1304-7280

Diabetes mellitus

immunohistochemistry

kidney

liver

Rheum ribes

TLR2

TLR4Böbrek

diyabetes mellitus

immunohistokimya

karaciğer

Rheum ribes

TLR2

TLR4

Sayı

Makale detayları

Yazarlar: Ayhan Akgün | Turgay Deprem

Yayıncı: Erciyes University

Ana Başlıklar: Veteriner Histoloji ve Embriyolojisi

Dergi: Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.21 Issue 3, pp.148-154

Belge Tipi: Makale

Yayın Tarihi: 2/2024

Belge Dili: Türkçe

Document ID (Org No.): 2001332601

pISSN: 1304-7280

Sayı

Yazarlar:

Ayhan Akgün | Turgay Deprem

Yayıncı:

Erciyes University

Ana Başlıklar:

Veteriner Histoloji ve Embriyolojisi

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.21 Issue 3, pp.148-154

Belge Tipi:

Makale

Yayın Tarihi:

2/2024

Belge Dili:

Türkçe

Document ID (Org No.):

2001332601

pISSN:

1304-7280