The effects of chard extract against streptozotocin-induced erectile dysfunction in rats

Mustafa Aydın; Özlem Saçan; Levent Kabasakal; Şule Çetinel; Mustafa Kadıhasanoglu; Muammer Kendirci; Refiye Yanardağ; Göksel Şener

Makale detayları

The effects of chard extract against streptozotocin-induced erectile dysfunction in rats

Yazarlar:

Mustafa Aydın | Özlem Saçan | Levent Kabasakal | Şule Çetinel | Mustafa Kadıhasanoglu | Muammer Kendirci | Refiye Yanardağ | Göksel Şener

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.12-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318870

pISSN:

2587-2087

Anahtar kelimeler:

Özet:

 Background and Aims: To analyze the potential therapeutic effects of chard against streptozotocin STZ -induced erectile dysfunction ED and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats 250-300g were allocated into groups as follows: control, diabetic, diabetic  chard, and diabetic  insulin. In order to induce diabetes, rats were given 65 mgkg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 gkg for 45 days beginning on 15th days. Sixty days after STZ injection, intracavernosal pressure ICP was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine ADMAlevels, and lactate dehydrogenase LDH activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence CL, malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes-induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.

 Background and Aims: To analyze the potential therapeutic effects of chard against streptozotocin STZ -induced erectile dysfunction ED and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats 250-300g were allocated into groups as follows: control, diabetic, diabetic  chard, and diabetic  insulin. In order to induce diabetes, rats were given 65 mgkg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 gkg for 45 days beginning on 15th days. Sixty days after STZ injection, intracavernosal pressure ICP was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine ADMAlevels, and lactate dehydrogenase LDH activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence CL, malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes-induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.

 Background and Aims: To analyze the potential therapeutic effects of chard against streptozotocin STZ -induced erectile dysfunction ED and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats 250-300g were allocated into groups as follows: control, diabetic, diabetic  chard, and diabetic  insulin. In order to induce diabetes, rats were given 65 mgkg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 gkg for 45 days beginning on 15th days. Sixty days after STZ injection, intracavernosal pressure ICP was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine ADMAlevels, and lactate dehydrogenase LDH activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence CL, malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes-induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.

 Background and Aims: To analyze the potential therapeutic effects of chard against streptozotocin STZ -induced erectile dysfunction ED and oxidative damage in the corpus cavernousum in rats. Materials and Methods: In this study, Sprague-Dawley rats 250-300g were allocated into groups as follows: control, diabetic, diabetic  chard, and diabetic  insulin. In order to induce diabetes, rats were given 65 mgkg intraperitoneal streptozotocin. Chard extract was given orally at a dose 2 gkg for 45 days beginning on 15th days. Sixty days after STZ injection, intracavernosal pressure ICP was measured and rats were decapitated. Blood samples were obtained for glucose, asymmetric dimethylarginine ADMAlevels, and lactate dehydrogenase LDH activity while cavernous tissues were taken to analyze luminol and lucigenin chemiluminescence CL, malondialdehyde and glutathione and along with histological analysis. Results: The results revealed that diabetes caused significant decreases in cavernosal tissue glutathione levels, while luminol and lucigenin CL, and malondialdehyde levels were significantly elevated. Plasma glucose, ADMA levels, and LDH activity were also found to be increased in diabetic group. On the other hand, both chard extract and insulin treatment reversed these biochemical parameters significantly. Furthermore, it was found that the ICP value examined for evaluating erectile functions were lower in the diabetic group, but increased in both treatment groups which were similar to the control values. Conclusion: According to our results, chard extract, similar to insulin, reduced diabetes-induced oxidative damage in cavernosal tissue and protected erectile functions. This effects may be attributed its hypoglycemic and antioxidant properties.

Makale detayları

The effects of chard extract against streptozotocin-induced erectile dysfunction in rats

Yazarlar:

Mustafa Aydın | Özlem Saçan | Levent Kabasakal | Şule Çetinel | Mustafa Kadıhasanoglu | Muammer Kendirci | Refiye Yanardağ | Göksel Şener

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.12-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318870

pISSN:

2587-2087

Chard extract

diabetes mellitus

erectile dysfunction

oxidative damage

Sayı

Makale detayları

The effects of chard extract against streptozotocin-induced erectile dysfunction in rats

Yazarlar: Mustafa Aydın | Özlem Saçan | Levent Kabasakal | Şule Çetinel | Mustafa Kadıhasanoglu | Muammer Kendirci | Refiye Yanardağ | Göksel Şener

Yayıncı: Istanbul University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences

Dergi: İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.12-19

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001318870

pISSN: 2587-2087

Chard extract

diabetes mellitus

erectile dysfunction

oxidative damage

Sayı

Yazarlar:

Mustafa Aydın | Özlem Saçan | Levent Kabasakal | Şule Çetinel | Mustafa Kadıhasanoglu | Muammer Kendirci | Refiye Yanardağ | Göksel Şener

Yayıncı:

Istanbul University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

İstanbul Journal of Pharmacy Vol.54 Issue 1, pp.12-19

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001318870

pISSN:

2587-2087