Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situ hybridization of rDNA sites and conventional stainings

Arbi GUETAT; Marcella ROSATO; Josep A. ROSSELLÃ"; Mohamed BOUSSAID

Makale detayları

Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situ hybridization of rDNA sites and conventional stainings

Yazarlar:

Arbi GUETAT | Marcella ROSATO | Josep A. ROSSELLÃ" | Mohamed BOUSSAID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.796-807

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815693

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

In North Africa, Allium roseum L. Has been treated as a polymorphic species comprising 12 different taxa. More than 80 individuals in the Allium roseum complex were sampled from 20 populations at different localities in Tunisia and investigated karyologically. Basic chromosome number was confirmed as x = 8. Three cytotypes, diploid (2n = 2x = 16), triploid (2n = 3x = 24), and tetraploid (2n = 4x = 32), were found. Polyploidy was recorded for the first time for this species in Tunisia. Most of the populations were diploid (17 populations), whereas two populations were tetraploid and only one was triploid. The chromosome set of studied populations is nonuniform at the species level and within cytotypes. The 45S and 5S ribosomal genes (rDNA) were visualized by fluorescent in situ hybridization for the A. Roseum polyploid complex. The diploid showed three loci 45S rDNA; the triploid and tetraploid showed 9 and 12 signals, respectively. However, 5S rDNA showed 2 signals for the diploid genome and 4 signals for both triploid and tetraploid. The 45S rDNA results showed intra- and interpopulational numerical variation. However, the data obtained with the 5S rDNA genes revealed a highly conserved distribution of these ribosomal families in the chromosomes of individuals from each population, as well as among the analyzed populations.

 

In North Africa, Allium roseum L. Has been treated as a polymorphic species comprising 12 different taxa. More than 80 individuals in the Allium roseum complex were sampled from 20 populations at different localities in Tunisia and investigated karyologically. Basic chromosome number was confirmed as x = 8. Three cytotypes, diploid (2n = 2x = 16), triploid (2n = 3x = 24), and tetraploid (2n = 4x = 32), were found. Polyploidy was recorded for the first time for this species in Tunisia. Most of the populations were diploid (17 populations), whereas two populations were tetraploid and only one was triploid. The chromosome set of studied populations is nonuniform at the species level and within cytotypes. The 45S and 5S ribosomal genes (rDNA) were visualized by fluorescent in situ hybridization for the A. Roseum polyploid complex. The diploid showed three loci 45S rDNA; the triploid and tetraploid showed 9 and 12 signals, respectively. However, 5S rDNA showed 2 signals for the diploid genome and 4 signals for both triploid and tetraploid. The 45S rDNA results showed intra- and interpopulational numerical variation. However, the data obtained with the 5S rDNA genes revealed a highly conserved distribution of these ribosomal families in the chromosomes of individuals from each population, as well as among the analyzed populations.

Makale detayları

Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situ hybridization of rDNA sites and conventional stainings

Yazarlar:

Arbi GUETAT | Marcella ROSATO | Josep A. ROSSELLÃ" | Mohamed BOUSSAID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.796-807

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815693

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Allium roseum L.

basic chromosome number

karyotype

ploidy level

fluorescent in situ hybridization

5S and 45S rDNA

Sayı

Makale detayları

Karyotype analysis in Allium roseum L. (Alliaceae) using fluorescent in situ hybridization of rDNA sites and conventional stainings

Yazarlar: Arbi GUETAT | Marcella ROSATO | Josep A. ROSSELLÃ" | Mohamed BOUSSAID

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.796-807

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000815693

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Allium roseum L.

basic chromosome number

karyotype

ploidy level

fluorescent in situ hybridization

5S and 45S rDNA

Sayı

Yazarlar:

Arbi GUETAT | Marcella ROSATO | Josep A. ROSSELLÃ" | Mohamed BOUSSAID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 5, pp.796-807

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815693

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X