A calorimetric study of the interaction of silver ions with jack bean urease

Ali Akbar SABOURY; Elahe POORAKBAR; Gholamreza Rezaei BEHBEHANI

Makale detayları

A calorimetric study of the interaction of silver ions with jack bean urease

Yazarlar:

Ali Akbar SABOURY | Elahe POORAKBAR | Gholamreza Rezaei BEHBEHANI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2010 Vol.34 Issue 4, pp.631-638

Belge Tipi:

Makale

Yayın Tarihi:

3/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000728034

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 

A thermodynamic study of silver ions by jack bean urease  (JBU) was carried out at 2 temperatures, 27 and 37 °C, in Tris buffer  (30 mM; pH 7.0) using isothermal titration calorimetry (ITC).  There  was a set of 12 identical and noninteracting binding sites for the  silver ions.  The intrinsic dissociation equilibrium constant and the  molar enthalpy of binding were 185 m M and -16.7 kJ mol-1 at 27  °C, and 229 m M and -16.3 kJ mol-1 at 37 °C, respectively.  The  molar entropy of binding was +15.7 J K-1 mol-1 at 27 °C and  +17.1 J K-1 mol-1 at 37 °C. Hence, the binding process of silver  ions to JBU is not only enthalpy driven but is also entropy driven,  and the role of entropy should be made more effective by increasing  the temperature.

 

A thermodynamic study of silver ions by jack bean urease  (JBU) was carried out at 2 temperatures, 27 and 37 °C, in Tris buffer  (30 mM; pH 7.0) using isothermal titration calorimetry (ITC).  There  was a set of 12 identical and noninteracting binding sites for the  silver ions.  The intrinsic dissociation equilibrium constant and the  molar enthalpy of binding were 185 m M and -16.7 kJ mol-1 at 27  °C, and 229 m M and -16.3 kJ mol-1 at 37 °C, respectively.  The  molar entropy of binding was +15.7 J K-1 mol-1 at 27 °C and  +17.1 J K-1 mol-1 at 37 °C. Hence, the binding process of silver  ions to JBU is not only enthalpy driven but is also entropy driven,  and the role of entropy should be made more effective by increasing  the temperature.

Makale detayları

A calorimetric study of the interaction of silver ions with jack bean urease

Yazarlar: Ali Akbar SABOURY | Elahe POORAKBAR | Gholamreza Rezaei BEHBEHANI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Chemistry 2010 Vol.34 Issue 4, pp.631-638

Belge Tipi: Makale

Yayın Tarihi: 3/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000728034

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Ali Akbar SABOURY | Elahe POORAKBAR | Gholamreza Rezaei BEHBEHANI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Chemistry 2010 Vol.34 Issue 4, pp.631-638

Belge Tipi:

Makale

Yayın Tarihi:

3/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000728034

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527