Determination of N-acetylcysteine in the presence of ciprofloxacin or levofloxacin in microparticulate dry powder inhalers

Yağmur AKDAĞ; Selma ŞAHİN; Emirhan NEMUTLU; Levent ÖNER

Makale detayları

Determination of N-acetylcysteine in the presence of ciprofloxacin or levofloxacin in microparticulate dry powder inhalers

Yazarlar:

Yağmur AKDAĞ | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı:

TUBITAK

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi:

Makale

Yayın Tarihi:

6/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725674

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527

Özet:

 


A fast and easy method was validated for simultaneous determination of ciprofloxacin hydrochloride monohydrate(CP), levofloxacin hemihydrate (LV), and N-acetylcysteine (NAC) in samples. The analysis was performed on aC18 column (250 × 4.6 mm, 5 μm) (Inertsil ODS-3V) using an isocratic elution method with a mobile phase composedof 25 mM KH2 PO4 (pH 3.0) and methanol (72:28, v/v) at a flow rate of 1 mL/min. UV detection was performed at 214nm for NAC and 293 nm for CP and LV. The method was validated for linearity, accuracy, precision (repeatability andreproducibility), specificity, sensitivity, and stability. The calibration study using several media demonstrated that thecalibration curves were linear for all compounds in all media (R2 >0.9993). The limit of detection was 0.098 μg/mL forCP, 0.049 μg/mL for LV, and 0.487 μg/mL for NAC. The limit of quantification was 0.328 μg/mL for CP, 0.165 μg/mLfor LV, and 1.624 μg/mL for NAC. Precision and accuracy values of the method fulfilled the required limits. All theseoutcomes demonstrate that the validated HPLC method is appropriate for simultaneous analysis of CP, LV, and NACin samples for content uniformity of dry powder inhaler and permeability studies.

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Makale detayları

Determination of N-acetylcysteine in the presence of ciprofloxacin or levofloxacin in microparticulate dry powder inhalers

Yazarlar: Yağmur AKDAĞ | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı: TUBITAK

Ana Başlıklar: Uygulamalı fizik

Dergi: Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi: Makale

Yayın Tarihi: 6/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000725674

Sıklık: İki Aylık

eISSN: 1303-6130

pISSN: 1300-0527

Sayı

Yazarlar:

Yağmur AKDAĞ | Selma ŞAHİN | Emirhan NEMUTLU | Levent ÖNER

Yayıncı:

TUBITAK

Ana Başlıklar:

Uygulamalı fizik

Dergi:

Turkish Journal of Chemistry 2019 Vol.43 Issue 3, pp.846-856

Belge Tipi:

Makale

Yayın Tarihi:

6/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000725674

Sıklık:

İki Aylık

eISSN:

1303-6130

pISSN:

1300-0527