Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yakup AKKOÇ; S. Seyhan TÜKEL

doi:https://doi.org/10.32571/ijct.420772

Makale detayları

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yazarlar:

Yakup AKKOÇ | S. Seyhan TÜKEL

Yayıncı:

İbrahim DEMİRTAŞ

Ana Başlıklar:

Kimya mühendisliği

Dergi:

International Journal of Chemistry and Technology 2018 Vol.2 Issue 1, pp.63-67

Belge Tipi:

Makale

Yayın Tarihi:

6/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000476898

Sıklık:

İki Yıllık

eISSN:

2602-277X

DOI:

https://doi.org/10.32571/ijct.420772

Özet:

 


In this study, in order
to obtain fructose syrup from starch, pullulanase (PUL), glucoamylase (GA) and
glucose isomerase (GI) were immobilized together with cross-linked enzyme
aggregates (CLEAs), and named as 'combi-CLEAs'. The values of Vmaxand Kmfrom
the kinetic parameters of combi-CLEAs [(GA + PUL) + GI] were found as 12      U g-1 and 0.1157 mg ml-1
in optimum conditions, respectively. After
50 uses, it was observed that the remaining activity of combi-CLEAs [GA + PUL +
GI] was preserved at 46%. The thermal stability of combi-CLEAs
[GA + PUL) + GI] was observed to be 44% and 30%, respectively, at 60 and 80 ° C
after 24 hours. At the end of 30 days, the
storage stabilities of combi-CLEAs [(GA + PUL) + GI] was determined as 56% and
13% at the temperatures of 4 and 25°C, respectively.


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Makale detayları

Co-Immobilization of pullulanase, glucoamylase and glucose isomerase together with cross-linked enzyme aggregates: Fructose conversion from three enzymes and starch in one step

Yazarlar: Yakup AKKOÇ | S. Seyhan TÜKEL

Yayıncı: İbrahim DEMİRTAŞ

Ana Başlıklar: Kimya mühendisliği

Dergi: International Journal of Chemistry and Technology 2018 Vol.2 Issue 1, pp.63-67

Belge Tipi: Makale

Yayın Tarihi: 6/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000476898

Sıklık: İki Yıllık

eISSN: 2602-277X

DOI: https://doi.org/10.32571/ijct.420772

Sayı

Yazarlar:

Yakup AKKOÇ | S. Seyhan TÜKEL

Yayıncı:

İbrahim DEMİRTAŞ

Ana Başlıklar:

Kimya mühendisliği

Dergi:

International Journal of Chemistry and Technology 2018 Vol.2 Issue 1, pp.63-67

Belge Tipi:

Makale

Yayın Tarihi:

6/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000476898

Sıklık:

İki Yıllık

eISSN:

2602-277X

DOI:

https://doi.org/10.32571/ijct.420772