In vitro inhibitory activity of Annona squamosa leaves against enzymes associated with metabolic disorders

Md. Rowshanul Habib

Makale detayları

In vitro inhibitory activity of Annona squamosa leaves against enzymes associated with metabolic disorders

Yazarlar:

Md. Rowshanul Habib

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1272-1280

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347546

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Annona squamosa L. is a popular medicinal plant of Bangladesh having several traditional uses. To give the scientific clarification for ethnomedicinal uses of this plant, this study was aimed to analyze the phytochemical profile of methanol extract of Annona squamosa leaves designated as MEAL and evaluate its inhibitory properties against α-amylase, pancreatic lipase, and angiotensin converting enzyme ACE. Different in vitro methods were used to perform enzyme inhibition assays and kinetic studies for MEAL and its phytochemical profile was analyzed by gas chromatography-mass spectroscopy GCMS. In enzyme inhibition assays, MEAL showed significant ACE inhibitory activity whereas it had moderate inhibition against pancreatic lipase. The IC50 of MEAL was found to be 9.26  0.71 μgmL and 6.90  0.58 μgmL for ACE and lipase enzymes, respectively. MEAL was also found as mixed type inhibitor of ACE in enzyme kinetic study. In case of α-amylase, MELA IC50: 608.8  7.02 μgmL showed poor inhibition activity when compared with standard drug acarbose IC50: 28.53  1.35 μgmL. In addition, presence of some compounds in MEAL identified by GC-MS, were also consistence with these enzyme inhibitory activities. Thus, this study proves the enzyme inhibitory abilities of Annona squamosa leaves for the first time and it might be a potential tool for the treatment of hypertension and obesity.

 Annona squamosa L. is a popular medicinal plant of Bangladesh having several traditional uses. To give the scientific clarification for ethnomedicinal uses of this plant, this study was aimed to analyze the phytochemical profile of methanol extract of Annona squamosa leaves designated as MEAL and evaluate its inhibitory properties against α-amylase, pancreatic lipase, and angiotensin converting enzyme ACE. Different in vitro methods were used to perform enzyme inhibition assays and kinetic studies for MEAL and its phytochemical profile was analyzed by gas chromatography-mass spectroscopy GCMS. In enzyme inhibition assays, MEAL showed significant ACE inhibitory activity whereas it had moderate inhibition against pancreatic lipase. The IC50 of MEAL was found to be 9.26  0.71 μgmL and 6.90  0.58 μgmL for ACE and lipase enzymes, respectively. MEAL was also found as mixed type inhibitor of ACE in enzyme kinetic study. In case of α-amylase, MELA IC50: 608.8  7.02 μgmL showed poor inhibition activity when compared with standard drug acarbose IC50: 28.53  1.35 μgmL. In addition, presence of some compounds in MEAL identified by GC-MS, were also consistence with these enzyme inhibitory activities. Thus, this study proves the enzyme inhibitory abilities of Annona squamosa leaves for the first time and it might be a potential tool for the treatment of hypertension and obesity.

 Annona squamosa L. is a popular medicinal plant of Bangladesh having several traditional uses. To give the scientific clarification for ethnomedicinal uses of this plant, this study was aimed to analyze the phytochemical profile of methanol extract of Annona squamosa leaves designated as MEAL and evaluate its inhibitory properties against α-amylase, pancreatic lipase, and angiotensin converting enzyme ACE. Different in vitro methods were used to perform enzyme inhibition assays and kinetic studies for MEAL and its phytochemical profile was analyzed by gas chromatography-mass spectroscopy GCMS. In enzyme inhibition assays, MEAL showed significant ACE inhibitory activity whereas it had moderate inhibition against pancreatic lipase. The IC50 of MEAL was found to be 9.26  0.71 μgmL and 6.90  0.58 μgmL for ACE and lipase enzymes, respectively. MEAL was also found as mixed type inhibitor of ACE in enzyme kinetic study. In case of α-amylase, MELA IC50: 608.8  7.02 μgmL showed poor inhibition activity when compared with standard drug acarbose IC50: 28.53  1.35 μgmL. In addition, presence of some compounds in MEAL identified by GC-MS, were also consistence with these enzyme inhibitory activities. Thus, this study proves the enzyme inhibitory abilities of Annona squamosa leaves for the first time and it might be a potential tool for the treatment of hypertension and obesity.

 Annona squamosa L. is a popular medicinal plant of Bangladesh having several traditional uses. To give the scientific clarification for ethnomedicinal uses of this plant, this study was aimed to analyze the phytochemical profile of methanol extract of Annona squamosa leaves designated as MEAL and evaluate its inhibitory properties against α-amylase, pancreatic lipase, and angiotensin converting enzyme ACE. Different in vitro methods were used to perform enzyme inhibition assays and kinetic studies for MEAL and its phytochemical profile was analyzed by gas chromatography-mass spectroscopy GCMS. In enzyme inhibition assays, MEAL showed significant ACE inhibitory activity whereas it had moderate inhibition against pancreatic lipase. The IC50 of MEAL was found to be 9.26  0.71 μgmL and 6.90  0.58 μgmL for ACE and lipase enzymes, respectively. MEAL was also found as mixed type inhibitor of ACE in enzyme kinetic study. In case of α-amylase, MELA IC50: 608.8  7.02 μgmL showed poor inhibition activity when compared with standard drug acarbose IC50: 28.53  1.35 μgmL. In addition, presence of some compounds in MEAL identified by GC-MS, were also consistence with these enzyme inhibitory activities. Thus, this study proves the enzyme inhibitory abilities of Annona squamosa leaves for the first time and it might be a potential tool for the treatment of hypertension and obesity.

Makale detayları

In vitro inhibitory activity of Annona squamosa leaves against enzymes associated with metabolic disorders

Yazarlar:

Md. Rowshanul Habib

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1272-1280

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347546

pISSN:

2630-6344

Annona squamosa

leaves

inhibition

α-Amylase

lipase

ACE

Sayı

Makale detayları

In vitro inhibitory activity of Annona squamosa leaves against enzymes associated with metabolic disorders

Yazarlar: Md. Rowshanul Habib

Yayıncı: Marmara University

Ana Başlıklar: Pharmaceutical Biochemistry

Dergi: Journal of Research in Pharmacy Vol.26 Issue 5, pp.1272-1280

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347546

pISSN: 2630-6344

Annona squamosa

leaves

inhibition

α-Amylase

lipase

ACE

Sayı

Yazarlar:

Md. Rowshanul Habib

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmaceutical Biochemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 5, pp.1272-1280

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347546

pISSN:

2630-6344