The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

ALPER KOÇYİĞİT; MESUT ÇEVİK; UĞUR ŞEN; MEHMET KURAN

Makale detayları

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

Yazarlar:

ALPER KOÇYİĞİT | MESUT ÇEVİK | UĞUR ŞEN | MEHMET KURAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765570

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo culture. The quality of bovine embryos, based on the number of blastocyst cells and the ratio of ICM to total blastomeres, was affected by the sources of macromolecules and their combinations with growth factors. Growth factors supplemented to SOFaa media with BSA and PVA significantly increased the number of ICM cells and the ratio of ICM cells to total number of cells. In conclusion, replacing BSA with PVA depressed the blastocyst rate and cell numbers, and the number of blastomeres and ICM and TE cell numbers were affected by both the type of macromolecule and the growth factor supplements.

 

This study was conducted to determine the effects of different macromolecule sources added to synthetic oviduct fluid (SOF) culture medium supplemented with growth factors on the development of bovine embryos and blastocyst morphology. Zygotes were distributed into 5 treatment groups. Cleavage, morula, and blastocyst rates were evaluated under a stereomicroscope. Trophectoderm (TE) and inner cell mass (ICM) cells were determined by differential staining method. It was found that bovine serum albumin (BSA), either alone or in combination with growth factors, as compared to the control or polyvinyl-alcohol (PVA) resulted in higher embryo yield and faster development during early bovine embryo culture. The quality of bovine embryos, based on the number of blastocyst cells and the ratio of ICM to total blastomeres, was affected by the sources of macromolecules and their combinations with growth factors. Growth factors supplemented to SOFaa media with BSA and PVA significantly increased the number of ICM cells and the ratio of ICM cells to total number of cells. In conclusion, replacing BSA with PVA depressed the blastocyst rate and cell numbers, and the number of blastomeres and ICM and TE cell numbers were affected by both the type of macromolecule and the growth factor supplements.

Makale detayları

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

Yazarlar:

ALPER KOÇYİĞİT | MESUT ÇEVİK | UĞUR ŞEN | MEHMET KURAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765570

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Bovine

embryo culture

blastocyst quality

macromolecules

growth factors

Sayı

Makale detayları

The effect of macromolecule and growth factor combinations on in vitrodevelopment of bovine embryos

Yazarlar: ALPER KOÇYİĞİT | MESUT ÇEVİK | UĞUR ŞEN | MEHMET KURAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000765570

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Bovine

embryo culture

blastocyst quality

macromolecules

growth factors

Sayı

Yazarlar:

ALPER KOÇYİĞİT | MESUT ÇEVİK | UĞUR ŞEN | MEHMET KURAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2015 Vol.39 Issue 3

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000765570

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128