n vivo wound-healing and in vitro antibacterial and antioxidant properties of Carduus adpressus extract

Ayşe Arzu Şakul; Mehmet Evren Okur; Ayşe Esra Karadağ; Benay Daylan; Ekrem Musa Özdemir; Mehmet Yalçın Günal

Makale detayları

n vivo wound-healing and in vitro antibacterial and antioxidant properties of Carduus adpressus extract

Yazarlar:

Ayşe Arzu Şakul | Mehmet Evren Okur | Ayşe Esra Karadağ | Benay Daylan | Ekrem Musa Özdemir | Mehmet Yalçın Günal

Yayıncı:

Marmara University

Ana Başlıklar:

Medical Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 2, pp.665-676

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347830

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Carduus adpressus has been used for its anti-hair loss effect in traditional folk medicine. The plant species is mainly distributed in Türkiye, Bulgaria, and the Western Caucasus. The studies on this specific plant in the genus Carduus is limited which remarks the significance of the current study. This study aims to investigate the antibacterial, antioxidant, and wound-healing properties of the methanolic extract of Carduus adpressus. Extract was obtained by maceration. The broth microdilution assay was performed on Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. DPPH and ABTS radical scavenging assays were performed to detect the antioxidant capacity. Wound-healing activity was tested using alloxan-induced diabetic BALB-c mice. 4 groups, control, vehicle, CAE and Carduus adpressus groups were treated with the relative agent for 10 days. Extract demonstrated 62.5 μgmL MIC against S. aureus and E. faecalis, and 125 μgmL MIC against P. aeruginosa and E. coli. ABTS assay showed higher antioxidant activity compared to the DPPH assay. Carduus adpressus group demonstrated strong regeneration, epithelisation, and angiogenesis compared to the control group on day 10. Additionally, expression of PDGF, VEGF, and collagen formation was increased in the Carduus adpressus group compared to the control group on day 10. Extract demonstrated strong antibacterial, antioxidant, and wound-healing activities which indicate that it could be a source in developing wound-healing agents.

 Carduus adpressus has been used for its anti-hair loss effect in traditional folk medicine. The plant species is mainly distributed in Türkiye, Bulgaria, and the Western Caucasus. The studies on this specific plant in the genus Carduus is limited which remarks the significance of the current study. This study aims to investigate the antibacterial, antioxidant, and wound-healing properties of the methanolic extract of Carduus adpressus. Extract was obtained by maceration. The broth microdilution assay was performed on Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. DPPH and ABTS radical scavenging assays were performed to detect the antioxidant capacity. Wound-healing activity was tested using alloxan-induced diabetic BALB-c mice. 4 groups, control, vehicle, CAE and Carduus adpressus groups were treated with the relative agent for 10 days. Extract demonstrated 62.5 μgmL MIC against S. aureus and E. faecalis, and 125 μgmL MIC against P. aeruginosa and E. coli. ABTS assay showed higher antioxidant activity compared to the DPPH assay. Carduus adpressus group demonstrated strong regeneration, epithelisation, and angiogenesis compared to the control group on day 10. Additionally, expression of PDGF, VEGF, and collagen formation was increased in the Carduus adpressus group compared to the control group on day 10. Extract demonstrated strong antibacterial, antioxidant, and wound-healing activities which indicate that it could be a source in developing wound-healing agents.

 Carduus adpressus has been used for its anti-hair loss effect in traditional folk medicine. The plant species is mainly distributed in Türkiye, Bulgaria, and the Western Caucasus. The studies on this specific plant in the genus Carduus is limited which remarks the significance of the current study. This study aims to investigate the antibacterial, antioxidant, and wound-healing properties of the methanolic extract of Carduus adpressus. Extract was obtained by maceration. The broth microdilution assay was performed on Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. DPPH and ABTS radical scavenging assays were performed to detect the antioxidant capacity. Wound-healing activity was tested using alloxan-induced diabetic BALB-c mice. 4 groups, control, vehicle, CAE and Carduus adpressus groups were treated with the relative agent for 10 days. Extract demonstrated 62.5 μgmL MIC against S. aureus and E. faecalis, and 125 μgmL MIC against P. aeruginosa and E. coli. ABTS assay showed higher antioxidant activity compared to the DPPH assay. Carduus adpressus group demonstrated strong regeneration, epithelisation, and angiogenesis compared to the control group on day 10. Additionally, expression of PDGF, VEGF, and collagen formation was increased in the Carduus adpressus group compared to the control group on day 10. Extract demonstrated strong antibacterial, antioxidant, and wound-healing activities which indicate that it could be a source in developing wound-healing agents.

 Carduus adpressus has been used for its anti-hair loss effect in traditional folk medicine. The plant species is mainly distributed in Türkiye, Bulgaria, and the Western Caucasus. The studies on this specific plant in the genus Carduus is limited which remarks the significance of the current study. This study aims to investigate the antibacterial, antioxidant, and wound-healing properties of the methanolic extract of Carduus adpressus. Extract was obtained by maceration. The broth microdilution assay was performed on Staphylococcus aureus, Enterococcus faecalis, Pseudomonas aeruginosa, and Escherichia coli. DPPH and ABTS radical scavenging assays were performed to detect the antioxidant capacity. Wound-healing activity was tested using alloxan-induced diabetic BALB-c mice. 4 groups, control, vehicle, CAE and Carduus adpressus groups were treated with the relative agent for 10 days. Extract demonstrated 62.5 μgmL MIC against S. aureus and E. faecalis, and 125 μgmL MIC against P. aeruginosa and E. coli. ABTS assay showed higher antioxidant activity compared to the DPPH assay. Carduus adpressus group demonstrated strong regeneration, epithelisation, and angiogenesis compared to the control group on day 10. Additionally, expression of PDGF, VEGF, and collagen formation was increased in the Carduus adpressus group compared to the control group on day 10. Extract demonstrated strong antibacterial, antioxidant, and wound-healing activities which indicate that it could be a source in developing wound-healing agents.

Makale detayları

n vivo wound-healing and in vitro antibacterial and antioxidant properties of Carduus adpressus extract

Yazarlar:

Ayşe Arzu Şakul | Mehmet Evren Okur | Ayşe Esra Karadağ | Benay Daylan | Ekrem Musa Özdemir | Mehmet Yalçın Günal

Yayıncı:

Marmara University

Ana Başlıklar:

Medical Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 2, pp.665-676

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347830

pISSN:

2630-6344

Antibacterial

antioxidant

Carduus adpressus

extract

BALB-c mice

wound-healing

Sayı

Makale detayları

n vivo wound-healing and in vitro antibacterial and antioxidant properties of Carduus adpressus extract

Yazarlar: Ayşe Arzu Şakul | Mehmet Evren Okur | Ayşe Esra Karadağ | Benay Daylan | Ekrem Musa Özdemir | Mehmet Yalçın Günal

Yayıncı: Marmara University

Ana Başlıklar: Medical Pharmacology

Dergi: Journal of Research in Pharmacy Vol.27 Issue 2, pp.665-676

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347830

pISSN: 2630-6344

Antibacterial

antioxidant

Carduus adpressus

extract

BALB-c mice

wound-healing

Sayı

Yazarlar:

Ayşe Arzu Şakul | Mehmet Evren Okur | Ayşe Esra Karadağ | Benay Daylan | Ekrem Musa Özdemir | Mehmet Yalçın Günal

Yayıncı:

Marmara University

Ana Başlıklar:

Medical Pharmacology

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 2, pp.665-676

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347830

pISSN:

2630-6344