Effects of green tea on ACE gene expression in rat liver in CCl4-induced cirrhosis

Syed Muhammad SHAHID; Rozeena SHAIKH; Sana SHAMIM; Muhammad ISMAIL; Tabassum MAHBOOB

Makale detayları

Effects of green tea on ACE gene expression in rat liver in CCl4-induced cirrhosis

Yazarlar:

Syed Muhammad SHAHID | Rozeena SHAIKH | Sana SHAMIM | Muhammad ISMAIL | Tabassum MAHBOOB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.586-591

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753667

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

This study was designed to investigate the beneficial effects of green tea administration on angiotensin converting enzyme (ACE) gene expression in CCl4-induced liver cirrhosis in rats.  Materials and methods: A total of 24 male Wistar rats were divided into 3 groups as: Group 1, normal untreated rats; Group 2, CCl4-induced cirrhotic rats; Group 3: CCl4-induced cirrhotic + green tea treated rats. The beneficial effects of green tea were measured by alanine aminotransferase (ALT), alkaline phosphatase (ALP), total and direct bilirubin level, tissue malondialdehyde (MDA), super oxide dismutase (SOD), and catalase (CAT). The genomic DNA was isolated from excised tissue to determine the ACE genotypes using specific primers. The ACE gene expression in liver tissue was assessed using the quantitative RT-PCR method.  Results: Liver cirrhosis was indicated by high plasma ALT, direct bilirubin level, tissue MDA, and low SOD. The antioxidant enzymes SOD and CAT were low (P < 0.01) in cirrhotic and green tea-treated rats. High activity (P < 0.01) of ALT was observed in green tea-treated cirrhotic rats. The total and direct bilirubin levels were high (P < 0.01) in CCl4-treated cirrhotic rats while they were low (P < 0.05) in green tea-treated cirrhotic rats. The tissue MDA was high (P < 0.01) in CCl4- and green tea-treated cirrhotic rats. ACE gene expression after 8 weeks of CCl4 treatment in cirrhotic rats was significantly high (P < 0.05), and was reversed in green tea-treated cirrhotic rats in comparison to controls.  Conclusion: The administration of green tea tries to correct the deteriorative biochemical and genetic changes during CCl4-induced liver cirrhosis in rats. The long-term consumption of green tea has beneficial effects on abnormally increased ACE gene activity during liver cirrhosis caused by CCl4 administration in rats.

 

This study was designed to investigate the beneficial effects of green tea administration on angiotensin converting enzyme (ACE) gene expression in CCl4-induced liver cirrhosis in rats.  Materials and methods: A total of 24 male Wistar rats were divided into 3 groups as: Group 1, normal untreated rats; Group 2, CCl4-induced cirrhotic rats; Group 3: CCl4-induced cirrhotic + green tea treated rats. The beneficial effects of green tea were measured by alanine aminotransferase (ALT), alkaline phosphatase (ALP), total and direct bilirubin level, tissue malondialdehyde (MDA), super oxide dismutase (SOD), and catalase (CAT). The genomic DNA was isolated from excised tissue to determine the ACE genotypes using specific primers. The ACE gene expression in liver tissue was assessed using the quantitative RT-PCR method.  Results: Liver cirrhosis was indicated by high plasma ALT, direct bilirubin level, tissue MDA, and low SOD. The antioxidant enzymes SOD and CAT were low (P < 0.01) in cirrhotic and green tea-treated rats. High activity (P < 0.01) of ALT was observed in green tea-treated cirrhotic rats. The total and direct bilirubin levels were high (P < 0.01) in CCl4-treated cirrhotic rats while they were low (P < 0.05) in green tea-treated cirrhotic rats. The tissue MDA was high (P < 0.01) in CCl4- and green tea-treated cirrhotic rats. ACE gene expression after 8 weeks of CCl4 treatment in cirrhotic rats was significantly high (P < 0.05), and was reversed in green tea-treated cirrhotic rats in comparison to controls.  Conclusion: The administration of green tea tries to correct the deteriorative biochemical and genetic changes during CCl4-induced liver cirrhosis in rats. The long-term consumption of green tea has beneficial effects on abnormally increased ACE gene activity during liver cirrhosis caused by CCl4 administration in rats.

Makale detayları

Effects of green tea on ACE gene expression in rat liver in CCl4-induced cirrhosis

Yazarlar:

Syed Muhammad SHAHID | Rozeena SHAIKH | Sana SHAMIM | Muhammad ISMAIL | Tabassum MAHBOOB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.586-591

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753667

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Green tea

liver cirrhosis

ALT

ALP

total and direct bilirubin

ACE gene

Sayı

Makale detayları

Effects of green tea on ACE gene expression in rat liver in CCl4-induced cirrhosis

Yazarlar: Syed Muhammad SHAHID | Rozeena SHAIKH | Sana SHAMIM | Muhammad ISMAIL | Tabassum MAHBOOB

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.586-591

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000753667

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Green tea

liver cirrhosis

ALT

ALP

total and direct bilirubin

ACE gene

Sayı

Yazarlar:

Syed Muhammad SHAHID | Rozeena SHAIKH | Sana SHAMIM | Muhammad ISMAIL | Tabassum MAHBOOB

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 4, pp.586-591

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753667

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144