Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods

Melis SESLİ; Esin AKBAY; Mehmet Ali ONUR

Makale detayları

Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods

Yazarlar:

Melis SESLİ | Esin AKBAY | Mehmet Ali ONUR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.537-547

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811173

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 


 
                         Decellularization is a process that involves the removal of cellular material from the tissues and organs while maintaining the
structural, functional, and mechanical properties of extracellular matrix. The purpose of this study was to carry out decellularization of
rat adipose tissue, diaphragm, and heart by using two different methods in order to compare their efficiency and investigate proliferation
profiles of rat adipose-tissue-derived mesenchymal stem cells (AdMSCs) on these scaffolds. Tissues were treated with an optimized
detergent-based decellularization (Method A) and a freeze-and-thaw-based decellularization (Method B). AdMSCs were then seeded
on scaffolds having a density of 2 × 105 cells/scaffold and AO/PI double-staining and MTT assays were performed in order to determine
cell viability. In this study, which is the first research comparing two methods of decellularization of an adipose tissue, diaphragm, and
heart scaffolds with AdMSCs, Method A provided efficient decellularization in these three tissues and it was shown that these porous
scaffolds were cyto-compatible for the cells. Method B caused severe tissue damage in diaphragm and insufficient decellularization in
heart whereas it also resulted in cyto-compatible adipose tissue scaffolds.
                    

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Makale detayları

Decellularization of rat adipose tissue, diaphragm, and heart: a comparison of two decellularization methods

Yazarlar: Melis SESLİ | Esin AKBAY | Mehmet Ali ONUR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.537-547

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811173

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Melis SESLİ | Esin AKBAY | Mehmet Ali ONUR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 6, pp.537-547

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811173

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152