Cytotoxic effect of Trigonella coerulescens subsp. ayvalikensis Erdoğan, Selvi & Tümen in prostate and colon cancer cell lines

Sümeyye Aydoğan Türkoğlu; Selami Selvi

Makale detayları

Cytotoxic effect of Trigonella coerulescens subsp. ayvalikensis Erdoğan, Selvi & Tümen in prostate and colon cancer cell lines

Yazarlar:

Sümeyye Aydoğan Türkoğlu | Selami Selvi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.700-709

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326701

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Trigonella coerulescens subsp. ayvalikensis is an annual local endemic taxon distributed only in AyvalıkBalıkesir district and evaluated in the Vulnerable Category VU. Trigonella L. is an important genus with medicinal and economic value in the Fabaceae family. Seeds of the genus Trigonella are known to contain several groups of secondary metabolites, the most abundant compounds being steroidal saponins, as well as flavones, isoflavones, and polysaccharides. In our study, the cytotoxic effect on two different cancer cell lines namely, PC-3 prostate and SW480 colon, was investigated by extracting Trigonella coerulescens subsp. ayvalikensis with different solvents. The cytotoxic effects of extracts obtained from plant seeds with different solvents hexane, methanol, ethanol, acetone were investigated. The MTT test was used to examine the cytotoxic effect, which was studied with PC-3 and SW480 cancer cell lines. The different concentrations 23.45 µgµL, 46.875 µgµL, 93.75 µgµL, 187.5 µgµL, 375 µgµL of seed extracts were applied to the cells at different times points 24h, 48h and 72h and absorbance was taken at 550 nm in the spectrophotometer. As a result of cytotoxic studies, it was observed that hexane extract had the most reducing effect on PC-3 compared to the control groups. In the SW480 cell line, a proliferative effect was observed in extracts prepared with methanol, hexane, and acetone in the early period of 24 hours. In the later period 72 hours, the extract prepared with hexane and acetone showed the most cytotoxic effect on SW480 cells.

 Trigonella coerulescens subsp. ayvalikensis is an annual local endemic taxon distributed only in AyvalıkBalıkesir district and evaluated in the Vulnerable Category VU. Trigonella L. is an important genus with medicinal and economic value in the Fabaceae family. Seeds of the genus Trigonella are known to contain several groups of secondary metabolites, the most abundant compounds being steroidal saponins, as well as flavones, isoflavones, and polysaccharides. In our study, the cytotoxic effect on two different cancer cell lines namely, PC-3 prostate and SW480 colon, was investigated by extracting Trigonella coerulescens subsp. ayvalikensis with different solvents. The cytotoxic effects of extracts obtained from plant seeds with different solvents hexane, methanol, ethanol, acetone were investigated. The MTT test was used to examine the cytotoxic effect, which was studied with PC-3 and SW480 cancer cell lines. The different concentrations 23.45 µgµL, 46.875 µgµL, 93.75 µgµL, 187.5 µgµL, 375 µgµL of seed extracts were applied to the cells at different times points 24h, 48h and 72h and absorbance was taken at 550 nm in the spectrophotometer. As a result of cytotoxic studies, it was observed that hexane extract had the most reducing effect on PC-3 compared to the control groups. In the SW480 cell line, a proliferative effect was observed in extracts prepared with methanol, hexane, and acetone in the early period of 24 hours. In the later period 72 hours, the extract prepared with hexane and acetone showed the most cytotoxic effect on SW480 cells.

 Trigonella coerulescens subsp. ayvalikensis is an annual local endemic taxon distributed only in AyvalıkBalıkesir district and evaluated in the Vulnerable Category VU. Trigonella L. is an important genus with medicinal and economic value in the Fabaceae family. Seeds of the genus Trigonella are known to contain several groups of secondary metabolites, the most abundant compounds being steroidal saponins, as well as flavones, isoflavones, and polysaccharides. In our study, the cytotoxic effect on two different cancer cell lines namely, PC-3 prostate and SW480 colon, was investigated by extracting Trigonella coerulescens subsp. ayvalikensis with different solvents. The cytotoxic effects of extracts obtained from plant seeds with different solvents hexane, methanol, ethanol, acetone were investigated. The MTT test was used to examine the cytotoxic effect, which was studied with PC-3 and SW480 cancer cell lines. The different concentrations 23.45 µgµL, 46.875 µgµL, 93.75 µgµL, 187.5 µgµL, 375 µgµL of seed extracts were applied to the cells at different times points 24h, 48h and 72h and absorbance was taken at 550 nm in the spectrophotometer. As a result of cytotoxic studies, it was observed that hexane extract had the most reducing effect on PC-3 compared to the control groups. In the SW480 cell line, a proliferative effect was observed in extracts prepared with methanol, hexane, and acetone in the early period of 24 hours. In the later period 72 hours, the extract prepared with hexane and acetone showed the most cytotoxic effect on SW480 cells.

 Trigonella coerulescens subsp. ayvalikensis is an annual local endemic taxon distributed only in AyvalıkBalıkesir district and evaluated in the Vulnerable Category VU. Trigonella L. is an important genus with medicinal and economic value in the Fabaceae family. Seeds of the genus Trigonella are known to contain several groups of secondary metabolites, the most abundant compounds being steroidal saponins, as well as flavones, isoflavones, and polysaccharides. In our study, the cytotoxic effect on two different cancer cell lines namely, PC-3 prostate and SW480 colon, was investigated by extracting Trigonella coerulescens subsp. ayvalikensis with different solvents. The cytotoxic effects of extracts obtained from plant seeds with different solvents hexane, methanol, ethanol, acetone were investigated. The MTT test was used to examine the cytotoxic effect, which was studied with PC-3 and SW480 cancer cell lines. The different concentrations 23.45 µgµL, 46.875 µgµL, 93.75 µgµL, 187.5 µgµL, 375 µgµL of seed extracts were applied to the cells at different times points 24h, 48h and 72h and absorbance was taken at 550 nm in the spectrophotometer. As a result of cytotoxic studies, it was observed that hexane extract had the most reducing effect on PC-3 compared to the control groups. In the SW480 cell line, a proliferative effect was observed in extracts prepared with methanol, hexane, and acetone in the early period of 24 hours. In the later period 72 hours, the extract prepared with hexane and acetone showed the most cytotoxic effect on SW480 cells.

Makale detayları

Cytotoxic effect of Trigonella coerulescens subsp. ayvalikensis Erdoğan, Selvi & Tümen in prostate and colon cancer cell lines

Yazarlar:

Sümeyye Aydoğan Türkoğlu | Selami Selvi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.700-709

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326701

pISSN:

2148-6905

Trigonella

Prostate cancer

Colon cancer

CytotoxicityTrigonella

Prostate cancer

Colon cancer

Cytotoxicity

Sayı

Makale detayları

Yazarlar: Sümeyye Aydoğan Türkoğlu | Selami Selvi

Yayıncı: İzzet KARA

Ana Başlıklar: Plant Biochemistry

Dergi: International Journal of Secondary Metabolite Vol.11 Issue 4, pp.700-709

Belge Tipi: Makale

Yayın Tarihi: 3/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001326701

pISSN: 2148-6905

Sayı

Yazarlar:

Sümeyye Aydoğan Türkoğlu | Selami Selvi

Yayıncı:

İzzet KARA

Ana Başlıklar:

Plant Biochemistry

Dergi:

International Journal of Secondary Metabolite Vol.11 Issue 4, pp.700-709

Belge Tipi:

Makale

Yayın Tarihi:

3/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001326701

pISSN:

2148-6905