OPTIMIZATION FORMULATION OF INSULIN-LOADED POLY-CAPROLACTONE NANOPARTICLES FOR CHRONIC WOUND HEALING USING BOX-BEHNKEN DESIGN

Tamer Tekin; Ayşegül Yıldız; Fatma Nur Tuğcu Demiröz; Füsun Acartürk

Makale detayları

OPTIMIZATION FORMULATION OF INSULIN-LOADED POLY-CAPROLACTONE NANOPARTICLES FOR CHRONIC WOUND HEALING USING BOX-BEHNKEN DESIGN

Yazarlar:

Tamer Tekin | Ayşegül Yıldız | Fatma Nur Tuğcu Demiröz | Füsun Acartürk

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.900-914

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356415

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: The aim of this study is to optimize the formulation of insulin-loaded poly-caprolactone nanoparticles INS-PCL-NP for use in chronic wound healing using Box-Behnken experimental design. Material and Method: The response of independent variables poly-caprolactone concentration, polyvinyl alcohol concentration and sonication time on dependent variables particle size, PDI and encapsulation efficiency were evaluated. Nanoparticle formulations were produced by the double emulsion wow-solvent evaporation method. The resulting formulations were characterized in terms of particle size, zeta potential, and encapsulation efficiency to determine the optimum formulation. Further analyses, including morphological analyses, Fourier-transform infrared spectroscopy FTIR, differential scanning calorimetry DSC, and in vitro drug release studies were conducted on the optimized formulation. Result and Discussion: According to the results of the characterization studies, it was observed that the optimized nanoparticles had a particle size of 618.511.2 nm, a PDI of 0.1940.021, a zeta potential of -8.211.1 mV and an encapsulation efficiency of 73.14.2. In morphological analysis, it was observed that the nanoparticles had a spherical structure. The optimized nanoparticle formulation showed a rapid release of 49.12.1 within the first 24 hours, followed by a controlled release for 168 hours.

 Objective: The aim of this study is to optimize the formulation of insulin-loaded poly-caprolactone nanoparticles INS-PCL-NP for use in chronic wound healing using Box-Behnken experimental design. Material and Method: The response of independent variables poly-caprolactone concentration, polyvinyl alcohol concentration and sonication time on dependent variables particle size, PDI and encapsulation efficiency were evaluated. Nanoparticle formulations were produced by the double emulsion wow-solvent evaporation method. The resulting formulations were characterized in terms of particle size, zeta potential, and encapsulation efficiency to determine the optimum formulation. Further analyses, including morphological analyses, Fourier-transform infrared spectroscopy FTIR, differential scanning calorimetry DSC, and in vitro drug release studies were conducted on the optimized formulation. Result and Discussion: According to the results of the characterization studies, it was observed that the optimized nanoparticles had a particle size of 618.511.2 nm, a PDI of 0.1940.021, a zeta potential of -8.211.1 mV and an encapsulation efficiency of 73.14.2. In morphological analysis, it was observed that the nanoparticles had a spherical structure. The optimized nanoparticle formulation showed a rapid release of 49.12.1 within the first 24 hours, followed by a controlled release for 168 hours.

 Objective: The aim of this study is to optimize the formulation of insulin-loaded poly-caprolactone nanoparticles INS-PCL-NP for use in chronic wound healing using Box-Behnken experimental design. Material and Method: The response of independent variables poly-caprolactone concentration, polyvinyl alcohol concentration and sonication time on dependent variables particle size, PDI and encapsulation efficiency were evaluated. Nanoparticle formulations were produced by the double emulsion wow-solvent evaporation method. The resulting formulations were characterized in terms of particle size, zeta potential, and encapsulation efficiency to determine the optimum formulation. Further analyses, including morphological analyses, Fourier-transform infrared spectroscopy FTIR, differential scanning calorimetry DSC, and in vitro drug release studies were conducted on the optimized formulation. Result and Discussion: According to the results of the characterization studies, it was observed that the optimized nanoparticles had a particle size of 618.511.2 nm, a PDI of 0.1940.021, a zeta potential of -8.211.1 mV and an encapsulation efficiency of 73.14.2. In morphological analysis, it was observed that the nanoparticles had a spherical structure. The optimized nanoparticle formulation showed a rapid release of 49.12.1 within the first 24 hours, followed by a controlled release for 168 hours.

 Amaç: Bu çalışmanın amacı kronik yara iyileşmesinde kullanılmak üzere insülin yüklü poli-kaprolakton nanopartiküllerinin INS-PCL-NP formülasyonunu Box-Behnken deneysel tasarımı kullanarak optimize etmektir. Gereç ve Yöntem: Bağımsız değişkenlerin poli-kaprolakton konsantrasyonu, polivinil alkol konsantrasyonu ve sonikasyon süresi bağımlı değişkenlere partikül boyutu, PDI ve kapsülleme verimliliği tepkisi değerlendirilmiştir. Nanopartikül formülasyonları çift emülsiyon sys-çözücü buharlaştırma yöntemi ile üretilmiştir. Optimum formülasyonu belirlemek için nanopartiküller partikül boyutu, zeta potansiyeli ve enkapsülasyon etkinliği açısından karakterize edilmiştir. Deneysel tasarıma uygun olarak belirlenen optimum nanopartikül formülasyonu ile morfolojik analizler, FTIR, DSC analizleri ve in vitro ilaç salım çalışmaları yapılmıştır. Sonuç ve Tartışma: Karakterizasyon çalışmalarının sonuçlarına göre, optimize edilmiş nanopartiküllerin 618.511.2 nm partikül boyutuna, 0.1940.021 PDIye, -8.211.1 mV zeta potansiyeline ve 73.14.2 enkapsülasyon etkinliğine sahip olduğu görülmüştür. Morfolojik analizde, nanopartiküllerin küresel bir yapıya sahip olduğu görülmüştür. Optimize edilmiş nanopartikül formülasyonu, ilk 24 saat içinde 49.12.1lik ilk salım, ardından 168 saat boyunca kontrollü salım göstermiştir.

Makale detayları

OPTIMIZATION FORMULATION OF INSULIN-LOADED POLY-CAPROLACTONE NANOPARTICLES FOR CHRONIC WOUND HEALING USING BOX-BEHNKEN DESIGN

Yazarlar:

Tamer Tekin | Ayşegül Yıldız | Fatma Nur Tuğcu Demiröz | Füsun Acartürk

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.900-914

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356415

pISSN:

1015-3918

Box-Behnken design

insulin

nanoparticle

polycaprolactoneBox-Behnken tasarımı

insülin

nanopartikül

polikaprolakton

Sayı

Makale detayları

Yazarlar: Tamer Tekin | Ayşegül Yıldız | Fatma Nur Tuğcu Demiröz | Füsun Acartürk

Yayıncı: Ankara University

Ana Başlıklar: İlaç Dağıtım Teknolojileri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.900-914

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001356415

pISSN: 1015-3918

Sayı

Yazarlar:

Tamer Tekin | Ayşegül Yıldız | Fatma Nur Tuğcu Demiröz | Füsun Acartürk

Yayıncı:

Ankara University

Ana Başlıklar:

İlaç Dağıtım Teknolojileri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.49 Issue 3, pp.900-914

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001356415

pISSN:

1015-3918