Determination of Chemical Components in Different Essential Oils and Their In Vitro Antifungal Effects on Wet Bubble Disease (Mycogone perniciosa) in Cultivated Mushroom (Agaricus bisporus)

Esin Basım

Makale detayları

Determination of Chemical Components in Different Essential Oils and Their In Vitro Antifungal Effects on Wet Bubble Disease (Mycogone perniciosa) in Cultivated Mushroom (Agaricus bisporus)

Yazarlar:

Esin Basım

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.11-25

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359195

pISSN:

2791-8807

Anahtar kelimeler:

Özet:

 The chemical components of the essential oils EOs from cumin Cuminum cyminum L., laurel Laurus nobilis L., oregano Oreganum vulgare L., and thyme Thymbra spicata L., which are used as spices, were determined through GC-MS analysis. These EOs were tested in vitro for their antifungal effects against Mycogone perniciosa, the causal agent of wet bubble disease in cultivated mushrooms Agaricus bisporus Lange Sing.. The antifungal effects of various doses 1, 5, 10, 20, 30, 40, 50, 100, 200, 300, 400, 500, 1000 ppm of the essential oils from cumin, laurel, oregano, and thyme were examined in vitro to assess their inhibition of M. perniciosa mycelial growth. Sterile deionized water served as the control. A 5 mm diameter disc, taken with a cork borer from the colony edge of the 7-day-old pathogen fungus, was placed on Potato Dextrose Agar PDA medium. Different doses of the essential oils were added to the center of the petri dish lid. The dishes were wrapped with parafilm, inverted, and incubated at 27C. Notably, the lower doses 100 ppm of thyme oil and 200 ppm of oregano oil resulted in 100 inhibition of M. perniciosa mycelial growth. The antifungal effects of cumin and laurel oils varied depending on the doses used. Overall, the essential oils of cumin, laurel, oregano, and thyme show potential as natural control agents against the wet bubble disease pathogen, M. perniciosa. Further research on the practical application of these oils in direct cultivation of mushrooms is needed to explore their full potential.

 The chemical components of the essential oils EOs from cumin Cuminum cyminum L., laurel Laurus nobilis L., oregano Oreganum vulgare L., and thyme Thymbra spicata L., which are used as spices, were determined through GC-MS analysis. These EOs were tested in vitro for their antifungal effects against Mycogone perniciosa, the causal agent of wet bubble disease in cultivated mushrooms Agaricus bisporus Lange Sing.. The antifungal effects of various doses 1, 5, 10, 20, 30, 40, 50, 100, 200, 300, 400, 500, 1000 ppm of the essential oils from cumin, laurel, oregano, and thyme were examined in vitro to assess their inhibition of M. perniciosa mycelial growth. Sterile deionized water served as the control. A 5 mm diameter disc, taken with a cork borer from the colony edge of the 7-day-old pathogen fungus, was placed on Potato Dextrose Agar PDA medium. Different doses of the essential oils were added to the center of the petri dish lid. The dishes were wrapped with parafilm, inverted, and incubated at 27C. Notably, the lower doses 100 ppm of thyme oil and 200 ppm of oregano oil resulted in 100 inhibition of M. perniciosa mycelial growth. The antifungal effects of cumin and laurel oils varied depending on the doses used. Overall, the essential oils of cumin, laurel, oregano, and thyme show potential as natural control agents against the wet bubble disease pathogen, M. perniciosa. Further research on the practical application of these oils in direct cultivation of mushrooms is needed to explore their full potential.

 The chemical components of the essential oils EOs from cumin Cuminum cyminum L., laurel Laurus nobilis L., oregano Oreganum vulgare L., and thyme Thymbra spicata L., which are used as spices, were determined through GC-MS analysis. These EOs were tested in vitro for their antifungal effects against Mycogone perniciosa, the causal agent of wet bubble disease in cultivated mushrooms Agaricus bisporus Lange Sing.. The antifungal effects of various doses 1, 5, 10, 20, 30, 40, 50, 100, 200, 300, 400, 500, 1000 ppm of the essential oils from cumin, laurel, oregano, and thyme were examined in vitro to assess their inhibition of M. perniciosa mycelial growth. Sterile deionized water served as the control. A 5 mm diameter disc, taken with a cork borer from the colony edge of the 7-day-old pathogen fungus, was placed on Potato Dextrose Agar PDA medium. Different doses of the essential oils were added to the center of the petri dish lid. The dishes were wrapped with parafilm, inverted, and incubated at 27C. Notably, the lower doses 100 ppm of thyme oil and 200 ppm of oregano oil resulted in 100 inhibition of M. perniciosa mycelial growth. The antifungal effects of cumin and laurel oils varied depending on the doses used. Overall, the essential oils of cumin, laurel, oregano, and thyme show potential as natural control agents against the wet bubble disease pathogen, M. perniciosa. Further research on the practical application of these oils in direct cultivation of mushrooms is needed to explore their full potential.

 Baharat olarak tüketilen kimyon Cuminum cyminum L., defne Laurus nobilis L., kekik Oreganum vulgare L. ve karabaş kekiği Thymbra spicata L. uçucu yağlarının kimyasal bileşenleri GC-MSde belirlenmiş ve bu uçucu yağların antifungal etkileri, kültür mantarında Agaricus bisporus Lange Sing. ıslak kabarcık hastalığı etmeni olan Mycogone perniciosaya karşı in vitro olarak test edilmiştir. Kimyon, defne, kekik ve karabaş kekiği uçucu yağlarının farklı dozlarının 1, 5, 10, 20, 30, 40, 50, 100, 200, 300, 400, 500, 1000 ppm antifungal etkileri, M. pernisiosanın miselyal büyümesi üzerindeki inhibe edici etkisi açısından in vitro olarak araştırılmıştır. Kontrol olarak steril deiyonize su kullanılmıştır. 7 günlük patojen fungusa ait 5 mm çapındaki diskler mantar delici ile alınarak Patates Dekstroz Agar PDA besiyerine konulmuştur. Uçucu yağların farklı dozları, petri kabı kapağının ortasına pipetle konularak hemen petri kutuları parafilm ile sarılmış ve ayrıca petri kutuları ters çevrilerek 270Cde inkübe edilmiştir. Kekik yağının daha düşük dozları bile M. perniciosanın miselyal gelişimi üzerinde 100 antifungal etki göstermiştir. Kimyon ve defnenin M. perniciosa üzerindeki antifungal etkileri, kullanılan uçucu yağların dozlarına göre farklılık göstermiştir. Kimyon, defne, kekik ve karabaş kekiği uçucu yağları, ıslak kabarcık hastalığı patojeni M. perniciosayı kontrol etmekiçin doğal ajanlar olarak kullanılma potansiyeline sahiptir. Uçucu yağların pratik kullanımı, doğrudan kültür mantarları üzerinde gelecekte yapılacak detaylı çalışmalarla tamamlanmalıdır.

Makale detayları

Determination of Chemical Components in Different Essential Oils and Their In Vitro Antifungal Effects on Wet Bubble Disease (Mycogone perniciosa) in Cultivated Mushroom (Agaricus bisporus)

Yazarlar:

Esin Basım

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.11-25

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359195

pISSN:

2791-8807

Agaricus bisporus

Essential oils

GC-MS

Mycogone pernicosaAgaricus bisporus

Essensiyal Yağlar

GC-MS

Mycogone perniciosa

Sayı

Makale detayları

Yazarlar: Esin Basım

Yayıncı: Bingol University

Ana Başlıklar: Fitopatoloji

Dergi: Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.11-25

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359195

pISSN: 2791-8807

Sayı

Yazarlar:

Esin Basım

Yayıncı:

Bingol University

Ana Başlıklar:

Fitopatoloji

Dergi:

Uluslararası Gıda Tarım ve Hayvan Bilimleri Dergisi Vol.5 Issue 2, pp.11-25

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359195

pISSN:

2791-8807