Effects of Rainbow Trout (Oncorhynchus mykiss) Serum (RTS) on Replication of Infectious Pancreatic Necrosis Virus (IPNV)

Hamdi ÖĞÜT

Makale detayları

Effects of Rainbow Trout (Oncorhynchus mykiss) Serum (RTS) on Replication of Infectious Pancreatic Necrosis Virus (IPNV)

Yazarlar:

Hamdi ÖĞÜT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2005 Vol.29 Issue 2, pp.505-510

Belge Tipi:

Makale

Yayın Tarihi:

1/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000769126

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

A series of experiments were conducted to determine the effects of rainbow trout serum (RTS), taken from rainbow trout (Oncorhynchus mykiss) that have never experienced infectious pancreatic necrosis disease, on infectious pancreatic necrosis virus (IPNV) in vitro and in vivo. In vitro, 109 IPNV isolates belonging to various subtypes and having various degrees of virulence were passed in the presence and absence of 1% RTS on rainbow trout gonad (RTG-2) cell line. Moreover, brook trout (Salvelinus fontinalis) were challenged with four virulent Buhl subtype isolates passed 11 times in the presence and absence of 1% RTS. Avirulent isolates of IPNV were inhibited from replication on RTG-2 cells even after a single passage in the presence of 1% RTS, whereas isolates having a high degree of virulence were consistently resistant to RTS. Moreover, in vivo the level of inhibition was related with virulence of the virus (P < 0.0001). Serum inhibition of RTS on IPNV growth in vivo and in vitro is not dependent on the virus serotype. The effect of RTS presence on virus virulence in virus propagation was variable even in the same serotype.

 

A series of experiments were conducted to determine the effects of rainbow trout serum (RTS), taken from rainbow trout (Oncorhynchus mykiss) that have never experienced infectious pancreatic necrosis disease, on infectious pancreatic necrosis virus (IPNV) in vitro and in vivo. In vitro, 109 IPNV isolates belonging to various subtypes and having various degrees of virulence were passed in the presence and absence of 1% RTS on rainbow trout gonad (RTG-2) cell line. Moreover, brook trout (Salvelinus fontinalis) were challenged with four virulent Buhl subtype isolates passed 11 times in the presence and absence of 1% RTS. Avirulent isolates of IPNV were inhibited from replication on RTG-2 cells even after a single passage in the presence of 1% RTS, whereas isolates having a high degree of virulence were consistently resistant to RTS. Moreover, in vivo the level of inhibition was related with virulence of the virus (P < 0.0001). Serum inhibition of RTS on IPNV growth in vivo and in vitro is not dependent on the virus serotype. The effect of RTS presence on virus virulence in virus propagation was variable even in the same serotype.

Makale detayları

Effects of Rainbow Trout (Oncorhynchus mykiss) Serum (RTS) on Replication of Infectious Pancreatic Necrosis Virus (IPNV)

Yazarlar:

Hamdi ÖĞÜT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2005 Vol.29 Issue 2, pp.505-510

Belge Tipi:

Makale

Yayın Tarihi:

1/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000769126

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

IPNV

6S

replication

rainbow trout serum

RTS

Sayı

Makale detayları

Effects of Rainbow Trout (Oncorhynchus mykiss) Serum (RTS) on Replication of Infectious Pancreatic Necrosis Virus (IPNV)

Yazarlar: Hamdi ÖĞÜT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2005 Vol.29 Issue 2, pp.505-510

Belge Tipi: Makale

Yayın Tarihi: 1/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000769126

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

IPNV

6S

replication

rainbow trout serum

RTS

Sayı

Yazarlar:

Hamdi ÖĞÜT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2005 Vol.29 Issue 2, pp.505-510

Belge Tipi:

Makale

Yayın Tarihi:

1/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000769126

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128