Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Leyla BAHAR; Semra KAHRAMAN; Nazan ERAS; Caroline PIRKEVI

Makale detayları

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Yazarlar:

Leyla BAHAR | Semra KAHRAMAN | Nazan ERAS | Caroline PIRKEVI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.706-713

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752728

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Background/aim: The aim of this study was to compare the cellular properties of endometrial tissues from fertile patients and patients having at least 3 previous in vitro fertilization failures, during the implantation window. The ultrastructural evaluation of the endometrium in the implantation window may shed light on the complexity of the implantation failure paradigm.   Materials and methods: The study involved 23 women, 14 infertile with a clinical diagnosis of repeated implantation failure (RIF) and 9 fertile, defined as the control group. Endometrial samples were examined by transmission electron microscopy (TEM).   Results: In the control group, secretory vacuoles and cytoplasmic projections filled with secretory material, called pinopodes, were noted; microvilli were observed on some apical surfaces; and ciliated cells were absent. In the RIF group, the number of pinopodes was remarkably lower, with some of them being immature. Moreover, decidualization of stromal cells was not frequent and fewer epithelial cells with poor secretory vacuoles were discerned.   Conclusion: TEM analyses of endometrial samples from the RIF group revealed dramatic differences at the ultrastructural level compared to the controls, which may well be an underlying cause of their infertility.

 

Background/aim: The aim of this study was to compare the cellular properties of endometrial tissues from fertile patients and patients having at least 3 previous in vitro fertilization failures, during the implantation window. The ultrastructural evaluation of the endometrium in the implantation window may shed light on the complexity of the implantation failure paradigm.   Materials and methods: The study involved 23 women, 14 infertile with a clinical diagnosis of repeated implantation failure (RIF) and 9 fertile, defined as the control group. Endometrial samples were examined by transmission electron microscopy (TEM).   Results: In the control group, secretory vacuoles and cytoplasmic projections filled with secretory material, called pinopodes, were noted; microvilli were observed on some apical surfaces; and ciliated cells were absent. In the RIF group, the number of pinopodes was remarkably lower, with some of them being immature. Moreover, decidualization of stromal cells was not frequent and fewer epithelial cells with poor secretory vacuoles were discerned.   Conclusion: TEM analyses of endometrial samples from the RIF group revealed dramatic differences at the ultrastructural level compared to the controls, which may well be an underlying cause of their infertility.

Makale detayları

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Yazarlar:

Leyla BAHAR | Semra KAHRAMAN | Nazan ERAS | Caroline PIRKEVI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.706-713

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752728

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Endometrial receptivity

recurrent implantation failure

transmission electron microscopy

Sayı

Makale detayları

Comparison of endometrial biopsies of fertile women and womenwith repeated implantation failure at the ultrastructural level

Yazarlar: Leyla BAHAR | Semra KAHRAMAN | Nazan ERAS | Caroline PIRKEVI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.706-713

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000752728

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Endometrial receptivity

recurrent implantation failure

transmission electron microscopy

Sayı

Yazarlar:

Leyla BAHAR | Semra KAHRAMAN | Nazan ERAS | Caroline PIRKEVI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.706-713

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000752728

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144