The Effect of Kisspeptin-10 on Cisplatin-Induced Testicular Oxidative Stress

Gözde Arkalı; Tutku Can Acısu; Edanur Güler Ekmen; Meltem Sağıroğlu; Fatma Beril Koçyiğit; Mehmet Çay; Abdurrauf Yüce; Mesut Aksakal

Makale detayları

The Effect of Kisspeptin-10 on Cisplatin-Induced Testicular Oxidative Stress

Yazarlar:

Gözde Arkalı | Tutku Can Acısu | Edanur Güler Ekmen | Meltem Sağıroğlu | Fatma Beril Koçyiğit | Mehmet Çay | Abdurrauf Yüce | Mesut Aksakal

Yayıncı:

Balıkesir University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.184-191

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343768

pISSN:

2146-9601

Anahtar kelimeler:

Özet:

 Objective: Cisplatin, one of the anticarcinogenic drugs, causes damage to spermatogenic cells, sertoli cells and leydig cells. This study aimed to investigate the kisspeptin-10 effect against oxidative stress caused by cisplatin in male reproductive organs and its negative effects on spermatological parameters. Materials and Methods: In the experiment, 34 male Sprague Dawley rats were used. Rats were divided into 4 groups as control, cisplatin, Kisspeptin-10 and cisplatinkisspeptin-10. Cisplatin 5 mgkgsingle dose and Kisspeptin-10 50 nmolkg dose were administered intraperitoneally for 7 days. Results: In the cisplatin group, testicular malondialdehyde level increased p0.001, catalase enzyme activity p0.001 and glutathione level p0.01 decreased, sperm motility p0.05 and testicular p0.01 and seminal vesicle p0.001 weights decreased. In the cisplatin group administered kisspeptin-10, it was determined that testicular malondialdehyde level decreased p0.001, glutathione level increased p0.01, sperm motility increased p0.05, and testicular p0.01 and seminal vesicle p0.001 weights increased compared to the cisplatin group. Conclusion: As a result, it was revealed that Kisspeptin-10 showed improving effects on spermatological parameters by reducing oxidative stress that is effective in cisplatin-induced testicular damage.

 Objective: Cisplatin, one of the anticarcinogenic drugs, causes damage to spermatogenic cells, sertoli cells and leydig cells. This study aimed to investigate the kisspeptin-10 effect against oxidative stress caused by cisplatin in male reproductive organs and its negative effects on spermatological parameters. Materials and Methods: In the experiment, 34 male Sprague Dawley rats were used. Rats were divided into 4 groups as control, cisplatin, Kisspeptin-10 and cisplatinkisspeptin-10. Cisplatin 5 mgkgsingle dose and Kisspeptin-10 50 nmolkg dose were administered intraperitoneally for 7 days. Results: In the cisplatin group, testicular malondialdehyde level increased p0.001, catalase enzyme activity p0.001 and glutathione level p0.01 decreased, sperm motility p0.05 and testicular p0.01 and seminal vesicle p0.001 weights decreased. In the cisplatin group administered kisspeptin-10, it was determined that testicular malondialdehyde level decreased p0.001, glutathione level increased p0.01, sperm motility increased p0.05, and testicular p0.01 and seminal vesicle p0.001 weights increased compared to the cisplatin group. Conclusion: As a result, it was revealed that Kisspeptin-10 showed improving effects on spermatological parameters by reducing oxidative stress that is effective in cisplatin-induced testicular damage.

 Objective: Cisplatin, one of the anticarcinogenic drugs, causes damage to spermatogenic cells, sertoli cells and leydig cells. This study aimed to investigate the kisspeptin-10 effect against oxidative stress caused by cisplatin in male reproductive organs and its negative effects on spermatological parameters. Materials and Methods: In the experiment, 34 male Sprague Dawley rats were used. Rats were divided into 4 groups as control, cisplatin, Kisspeptin-10 and cisplatinkisspeptin-10. Cisplatin 5 mgkgsingle dose and Kisspeptin-10 50 nmolkg dose were administered intraperitoneally for 7 days. Results: In the cisplatin group, testicular malondialdehyde level increased p0.001, catalase enzyme activity p0.001 and glutathione level p0.01 decreased, sperm motility p0.05 and testicular p0.01 and seminal vesicle p0.001 weights decreased. In the cisplatin group administered kisspeptin-10, it was determined that testicular malondialdehyde level decreased p0.001, glutathione level increased p0.01, sperm motility increased p0.05, and testicular p0.01 and seminal vesicle p0.001 weights increased compared to the cisplatin group. Conclusion: As a result, it was revealed that Kisspeptin-10 showed improving effects on spermatological parameters by reducing oxidative stress that is effective in cisplatin-induced testicular damage.

 Amaç: Antikanserojen ilaçlardan biri olan sisplatin, spermatogenik hücrelerde, Sertoli hücrelerinde, Leydig hücrelerinde hasara yol açmaktadır. Bu çalışmada, sisplatinin erkek üreme organlarında neden olduğu oksidatif stres ve akabinde gelişen spermatolojik parametreler üzerindeki olumsuz etkilere karşı kisspeptin-10un etkisinin araştırılması amaçlandı. Gereç ve Yöntem: Deneyde 34 adet erkek Sprague Dawley sıçanı kullanıldı. Ratlar kontrol, sisplatin, Kisspeptin-10 ve sisplatinkisspeptin-10 olarak 4 gruba ayrıldı. Sisplatin 5 mgkgtek doz, Kisspeptin-10 ise 50 nmolkg dozda 7 gün boyunca intraperitoneal uygulandı. Bulgular: Sisplatin grubunda testikular malondialdehit düzeyinin arttığı p0.001, katalaz enzim aktivitesinin p0.001 ve glutatyon düzeyinin p0.01 azaldığı, sperm motilitesinin p0.05 ve testis p0.01 ile vezikula seminalis p0.001 üreme organ ağırlığının azaldığı belirlendi. CP grubu ile kıyaslandığında CPKiss-10 grubunda testikular malondialdehit düzeyinin azaldığı p0.001, glutatyon düzeyinin arttığı p0.01, sperm motilitesinin p0.05 ve testis p0.01 ile vezikula seminalis p0.001 üreme organ ağırlığının arttığı belirlendi. Sonuç: Kisspeptin-10un sisplatin kaynaklı testikular hasarda etkili olan oksidatif stresi azaltarak, spermatolojik parametreler üzerinde iyileştirici etkiler gösterdiği kanısına varılmıştır.

Makale detayları

The Effect of Kisspeptin-10 on Cisplatin-Induced Testicular Oxidative Stress

Yazarlar:

Gözde Arkalı | Tutku Can Acısu | Edanur Güler Ekmen | Meltem Sağıroğlu | Fatma Beril Koçyiğit | Mehmet Çay | Abdurrauf Yüce | Mesut Aksakal

Yayıncı:

Balıkesir University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.184-191

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343768

pISSN:

2146-9601

Cisplatin

Testes

Kisspeptin-10

Oxidative StressSisplatin

Testis

Kisspeptin-10

Oksidatif Stres

Sayı

Makale detayları

Yazarlar: Gözde Arkalı | Tutku Can Acısu | Edanur Güler Ekmen | Meltem Sağıroğlu | Fatma Beril Koçyiğit | Mehmet Çay | Abdurrauf Yüce | Mesut Aksakal

Yayıncı: Balıkesir University

Ana Başlıklar: Veteriner Anatomi ve Fizyoloji

Dergi: Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.184-191

Belge Tipi: Makale

Yayın Tarihi: 3/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343768

pISSN: 2146-9601

Sayı

Yazarlar:

Gözde Arkalı | Tutku Can Acısu | Edanur Güler Ekmen | Meltem Sağıroğlu | Fatma Beril Koçyiğit | Mehmet Çay | Abdurrauf Yüce | Mesut Aksakal

Yayıncı:

Balıkesir University

Ana Başlıklar:

Veteriner Anatomi ve Fizyoloji

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.14 Issue 1, pp.184-191

Belge Tipi:

Makale

Yayın Tarihi:

3/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343768

pISSN:

2146-9601