Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Polat İpek; Mehmet Fırat Baran; Reşit Yıldız; Abdulkerim Hatipoğlu

Makale detayları

Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Yazarlar:

Polat İpek | Mehmet Fırat Baran | Reşit Yıldız | Abdulkerim Hatipoğlu

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.399-407

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290052

pISSN:

2618-5946

Anahtar kelimeler:

Özet:

 This study was carried out to synthesize silver nanoparticles AgNPs from Arum dioscoridis AD leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible UV-vis Spectroscopy, Field Emission Scanning Electron Microscopy FE-SEM, Electron Dispersive X-ray EDX Spectroscopy, Fourier Transform Infrared FT-IR Spectroscopy, Transmission Electron Microscopy TEM, X-ray Diffractometer XRD, Atomic Force Microscopy AFM and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration MIC values mgL of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT 3-4,5-dimetiltiazol-2-il-2,5-difeniltetrazolium bromid assay, the half-maximal inhibitory concentrations IC50: μgmL of AD-AgNPs on human colon adenocarcinoma cell CACO-2, human breast cancer cell MCF-7, glioblastoma multiforme cell T98-G, and healthy human umbilical vein endothelial cell HUVEC lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.

 This study was carried out to synthesize silver nanoparticles AgNPs from Arum dioscoridis AD leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible UV-vis Spectroscopy, Field Emission Scanning Electron Microscopy FE-SEM, Electron Dispersive X-ray EDX Spectroscopy, Fourier Transform Infrared FT-IR Spectroscopy, Transmission Electron Microscopy TEM, X-ray Diffractometer XRD, Atomic Force Microscopy AFM and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration MIC values mgL of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT 3-4,5-dimetiltiazol-2-il-2,5-difeniltetrazolium bromid assay, the half-maximal inhibitory concentrations IC50: μgmL of AD-AgNPs on human colon adenocarcinoma cell CACO-2, human breast cancer cell MCF-7, glioblastoma multiforme cell T98-G, and healthy human umbilical vein endothelial cell HUVEC lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.

 This study was carried out to synthesize silver nanoparticles AgNPs from Arum dioscoridis AD leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible UV-vis Spectroscopy, Field Emission Scanning Electron Microscopy FE-SEM, Electron Dispersive X-ray EDX Spectroscopy, Fourier Transform Infrared FT-IR Spectroscopy, Transmission Electron Microscopy TEM, X-ray Diffractometer XRD, Atomic Force Microscopy AFM and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration MIC values mgL of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT 3-4,5-dimetiltiazol-2-il-2,5-difeniltetrazolium bromid assay, the half-maximal inhibitory concentrations IC50: μgmL of AD-AgNPs on human colon adenocarcinoma cell CACO-2, human breast cancer cell MCF-7, glioblastoma multiforme cell T98-G, and healthy human umbilical vein endothelial cell HUVEC lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.

 This study was carried out to synthesize silver nanoparticles AgNPs from Arum dioscoridis AD leaf extract and to investigate the cytotoxic and antipathogenic effects of them. The plant material had a reducing and stabilizing effect on the synthesized nanomaterial. During the plant-mediated synthesis of nanomaterials, no substances that would cause environmental pollution were used. For the structural characterization of AD-AgNPs, Ultraviolet-visible UV-vis Spectroscopy, Field Emission Scanning Electron Microscopy FE-SEM, Electron Dispersive X-ray EDX Spectroscopy, Fourier Transform Infrared FT-IR Spectroscopy, Transmission Electron Microscopy TEM, X-ray Diffractometer XRD, Atomic Force Microscopy AFM and Zetasizer analyses were performed. The produced AgNPs showed maximum surface plasmon resonance at 431.67 nm and had mostly spherical morphology. The zeta potential value of the nanomaterial was -9.76 mV and the average powder crystal size was 31.48 nm. The minimum inhibitory concentration MIC values mgL of AD-AgNPs on Staphylococcus aureus, Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Candida albicans were 0.25, 2.00, 0.125, 4.00, and 1.00, respectively. After 24 and 48 hours of application by MTT 3-4,5-dimetiltiazol-2-il-2,5-difeniltetrazolium bromid assay, the half-maximal inhibitory concentrations IC50: μgmL of AD-AgNPs on human colon adenocarcinoma cell CACO-2, human breast cancer cell MCF-7, glioblastoma multiforme cell T98-G, and healthy human umbilical vein endothelial cell HUVEC lines were determined as 2.977, 2.801, 5.694, 4.392; 2.115, 2.300, 2.612, 4.091, respectively.

Makale detayları

Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Yazarlar:

Polat İpek | Mehmet Fırat Baran | Reşit Yıldız | Abdulkerim Hatipoğlu

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.399-407

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290052

pISSN:

2618-5946

AgNPs

Arum dioscoridis extract

Cytotoxicity

Green chemistry

Pathogen microorganism

Sayı

Makale detayları

Biosynthesis of silver nanoparticles from Arum dioscoridis plant leaf aqueous extract: anticancer and antimicrobial properties

Yazarlar: Polat İpek | Mehmet Fırat Baran | Reşit Yıldız | Abdulkerim Hatipoğlu

Yayıncı: Gültekin ÖZDEMİR

Ana Başlıklar: Food Engineering

Dergi: International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.399-407

Belge Tipi: Makale

Yayın Tarihi: 6/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001290052

pISSN: 2618-5946

AgNPs

Arum dioscoridis extract

Cytotoxicity

Green chemistry

Pathogen microorganism

Sayı

Yazarlar:

Polat İpek | Mehmet Fırat Baran | Reşit Yıldız | Abdulkerim Hatipoğlu

Yayıncı:

Gültekin ÖZDEMİR

Ana Başlıklar:

Food Engineering

Dergi:

International Journal of Agriculture Environment and Food Sciences Vol.7 Issue 2, pp.399-407

Belge Tipi:

Makale

Yayın Tarihi:

6/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001290052

pISSN:

2618-5946