Evaluation of the Proliferative Effects of Hexagonal Boron Nitride Nanoparticles on Leukemia Cells and Leukemia Stem Cells

Neslihan Meriç; Fatih Kar; Ezgi Kar

Makale detayları

Evaluation of the Proliferative Effects of Hexagonal Boron Nitride Nanoparticles on Leukemia Cells and Leukemia Stem Cells

Yazarlar:

Neslihan Meriç | Fatih Kar | Ezgi Kar

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Eczacılık Biyokimyası

Dergi:

Osmangazi Tıp Dergisi Vol.47 Issue 1, pp.22-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331253

pISSN:

1305-4953

Anahtar kelimeler:

Özet:

 Leukemia is a malignant disease that affects the bone marrow, lymphatic system, spleen, and blood-forming organs, leading to an excessive proliferation of white blood cells. Current cancer treatments are often limited by drug resistance, highlighting the need for novel therapeutic strategies. Nanoparticles, including boron nitride BN nanomaterials, have shown promise in enhancing drug delivery and therapeutic efficacy due to their excellent physical and chemical properties. This study aimed to evaluate the cytotoxic effects of hexagonal boron nitride nanoparticles hBN NPs on leukemia cells and leukemia stem cells to explore their potential use in leukemia treatment.: hBN NPs were synthesized and characterized using X-ray powder diffraction XRD, Scanning Electron Microscopy SEM, and Transmission Electron Microscopy TEM. Leukemia cell lines HL-60 and CCRF-CEM and CD34 leukemia stem cells were treated with various hBN NPs. Cell viability was assessed using MTS assays, and flow cytometry was employed to analyze the expression of leukemia surface markers. The study found that hBN NPs did not exhibit significant anticancer properties; instead, they promoted cell proliferation in leukemia cells and stem cells. The CCRF-CEM CD34 cells showed resistance to hBN NPs treatment, which reduced the treatments therapeutic efficacy. The lack of cytotoxicity toward healthy cells suggests potential selectivity, yet the proliferative effects on leukemia cells indicate that hBN NPs may not be suitable for leukemia treatment. hBN NPs lack therapeutic potential for leukemia due to their proliferative effects on leukemia cells. Future studies should focus on developing combination therapies and exploring hBN NPs impact on other cell lines to identify potential synergistic strategies that could overcome resistance mechanisms in leukemia and other cancers.

 Leukemia is a malignant disease that affects the bone marrow, lymphatic system, spleen, and blood-forming organs, leading to an excessive proliferation of white blood cells. Current cancer treatments are often limited by drug resistance, highlighting the need for novel therapeutic strategies. Nanoparticles, including boron nitride BN nanomaterials, have shown promise in enhancing drug delivery and therapeutic efficacy due to their excellent physical and chemical properties. This study aimed to evaluate the cytotoxic effects of hexagonal boron nitride nanoparticles hBN NPs on leukemia cells and leukemia stem cells to explore their potential use in leukemia treatment.: hBN NPs were synthesized and characterized using X-ray powder diffraction XRD, Scanning Electron Microscopy SEM, and Transmission Electron Microscopy TEM. Leukemia cell lines HL-60 and CCRF-CEM and CD34 leukemia stem cells were treated with various hBN NPs. Cell viability was assessed using MTS assays, and flow cytometry was employed to analyze the expression of leukemia surface markers. The study found that hBN NPs did not exhibit significant anticancer properties; instead, they promoted cell proliferation in leukemia cells and stem cells. The CCRF-CEM CD34 cells showed resistance to hBN NPs treatment, which reduced the treatments therapeutic efficacy. The lack of cytotoxicity toward healthy cells suggests potential selectivity, yet the proliferative effects on leukemia cells indicate that hBN NPs may not be suitable for leukemia treatment. hBN NPs lack therapeutic potential for leukemia due to their proliferative effects on leukemia cells. Future studies should focus on developing combination therapies and exploring hBN NPs impact on other cell lines to identify potential synergistic strategies that could overcome resistance mechanisms in leukemia and other cancers.

 Leukemia is a malignant disease that affects the bone marrow, lymphatic system, spleen, and blood-forming organs, leading to an excessive proliferation of white blood cells. Current cancer treatments are often limited by drug resistance, highlighting the need for novel therapeutic strategies. Nanoparticles, including boron nitride BN nanomaterials, have shown promise in enhancing drug delivery and therapeutic efficacy due to their excellent physical and chemical properties. This study aimed to evaluate the cytotoxic effects of hexagonal boron nitride nanoparticles hBN NPs on leukemia cells and leukemia stem cells to explore their potential use in leukemia treatment.: hBN NPs were synthesized and characterized using X-ray powder diffraction XRD, Scanning Electron Microscopy SEM, and Transmission Electron Microscopy TEM. Leukemia cell lines HL-60 and CCRF-CEM and CD34 leukemia stem cells were treated with various hBN NPs. Cell viability was assessed using MTS assays, and flow cytometry was employed to analyze the expression of leukemia surface markers. The study found that hBN NPs did not exhibit significant anticancer properties; instead, they promoted cell proliferation in leukemia cells and stem cells. The CCRF-CEM CD34 cells showed resistance to hBN NPs treatment, which reduced the treatments therapeutic efficacy. The lack of cytotoxicity toward healthy cells suggests potential selectivity, yet the proliferative effects on leukemia cells indicate that hBN NPs may not be suitable for leukemia treatment. hBN NPs lack therapeutic potential for leukemia due to their proliferative effects on leukemia cells. Future studies should focus on developing combination therapies and exploring hBN NPs impact on other cell lines to identify potential synergistic strategies that could overcome resistance mechanisms in leukemia and other cancers.

 Lösemi, kemik iliğini, lenf sistemini, dalağı ve kan oluşturan organları etkileyen ve beyaz kan hücrelerinin aşırı çoğalmasına yol açan kötü huylu bir hastalıktır. Mevcut kanser tedavileri genellikle ilaç direnciyle sınırlıdır ve bu da yeni tedavi stratejilerine olan ihtiyacı vurgulamaktadır. Bor nitrür BN nanomalzemeleri de dahil olmak üzere nanopartiküller, mükemmel fiziksel ve kimyasal özellikleri nedeniyle ilaç iletimini ve tedavi edici etkinliği artırmada umut vadetmektedir. Bu çalışma, lösemi tedavisinde potansiyel kullanımlarını araştırmak için hekzagonal bor nitrür nanopartiküllerinin hBN NPleri lösemi hücreleri ve lösemi kök hücreleri üzerindeki sitotoksik etkilerini değerlendirmeyi amaçlamaktadır. hBN NPleri, X-ışını toz kırınımı XRD, Taramalı Elektron Mikroskobu SEM ve Transmisyon Elektron Mikroskobu TEM kullanılarak sentezlendi ve karakterize edildi. Lösemi hücre hatları HL-60 ve CCRF-CEM ve CD34 lösemi kök hücreleri çeşitli hBN NP konsantrasyonlarıyla tedavi edildi. Hücre canlılığı MTS analizleri kullanılarak değerlendirildi ve lösemi yüzey belirteçlerinin ekspresyonunu analiz etmek için akış sitometrisi kullanıldı. Çalışma, hBN NPlerinin önemli antikanser özellikleri göstermediğini; bunun yerine lösemi hücrelerinde ve kök hücrelerinde hücre çoğalmasını teşvik ettiğini buldu. CCRF-CEM CD34 hücreleri hBN NP tedavisine direnç gösterdi ve bu da tedavinin terapötik etkinliğini azalttı. Sağlıklı hücrelere karşı sitotoksisitenin olmaması potansiyel seçiciliği düşündürmektedir, ancak lösemi hücreleri üzerindeki çoğaltıcı etkiler hBN NPlerinin lösemi tedavisi için uygun olmayabileceğini göstermektedir. hBN NPleri lösemi hücreleri üzerindeki çoğaltıcı etkileri nedeniyle lösemi için terapötik potansiyele sahip değildir. Gelecekteki çalışmalar, lösemi ve diğer kanserlerde direnç mekanizmalarının üstesinden gelebilecek potansiyel sinerjistik stratejileri belirlemek için kombinasyon tedavileri geliştirmeye ve hBN NPlerinin diğer hücre hatları üzerindeki etkisini araştırmaya odaklanmalıdır.

Makale detayları

Evaluation of the Proliferative Effects of Hexagonal Boron Nitride Nanoparticles on Leukemia Cells and Leukemia Stem Cells

Yazarlar:

Neslihan Meriç | Fatih Kar | Ezgi Kar

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Eczacılık Biyokimyası

Dergi:

Osmangazi Tıp Dergisi Vol.47 Issue 1, pp.22-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331253

pISSN:

1305-4953

Leukemia

Hexagonal Boron Nitride Nanoparticles

CytotoxicityLösemi

Hekzagonal Bor Nitrür Nanopartikülleri

Sitotoksisite

Sayı

Makale detayları

Evaluation of the Proliferative Effects of Hexagonal Boron Nitride Nanoparticles on Leukemia Cells and Leukemia Stem Cells

Yazarlar: Neslihan Meriç | Fatih Kar | Ezgi Kar

Yayıncı: Eskişehir Osmangazi University

Ana Başlıklar: Eczacılık Biyokimyası

Dergi: Osmangazi Tıp Dergisi Vol.47 Issue 1, pp.22-31

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001331253

pISSN: 1305-4953

Leukemia

Hexagonal Boron Nitride Nanoparticles

CytotoxicityLösemi

Hekzagonal Bor Nitrür Nanopartikülleri

Sitotoksisite

Sayı

Yazarlar:

Neslihan Meriç | Fatih Kar | Ezgi Kar

Yayıncı:

Eskişehir Osmangazi University

Ana Başlıklar:

Eczacılık Biyokimyası

Dergi:

Osmangazi Tıp Dergisi Vol.47 Issue 1, pp.22-31

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001331253

pISSN:

1305-4953