Evaluation of preanalytical error processes in the microbiology laboratory and effect of training on these processes

Nihan Ceken; Hülya Duran; Tuğba Kula Atik; Esin Avcı

Makale detayları

Evaluation of preanalytical error processes in the microbiology laboratory and effect of training on these processes

Yazarlar:

Nihan Ceken | Hülya Duran | Tuğba Kula Atik | Esin Avcı

Yayıncı:

Pamukkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 3, pp.648-659

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001329360

pISSN:

1308-0865

Anahtar kelimeler:

Özet:

 Purpose: We aimed to calculate preanalytical error rates in the Medical Microbiology Laboratory of our hospital by the six-sigma method and examine the effect of training on error rates by comparing performances of processes before and after training. Materials and methods: All samples evaluated between 2016-2021 were retrospectively examined. Rejected samples, blood culture contamination rate and urine culture contamination rate were evaluated via Laboratory Error Classification System. The staff obtaining laboratory samples were trained by means of live classes during 2017, 2018 and 2019, and with online classes during 2021. Error rates and sigma levels were calculated before and after training. Results: 685591 samples were accepted by our laboratory; 1175 0.2 were rejected. The most frequent cause of rejection 53.4 was hemolysis of sample. The sigma levels showed hemolysis of the sample as the most frequent cause of rejection, with a value of 4.7 good performance. Among other quality indicators, the rate of urinary culture contamination was 11.4, and the rate of blood culture contamination was 3.5. The total sigma level of urine culture contamination was 2.9 unacceptable performance, and the total blood culture contamination was 3.5 minimal performance. Error rates had generally decreased after training, while an increase in performance at the sigma level was detected at all three indicators. Conclusion: In order to minimize preanalytical errors in the medical laboratory, the preanalytical process should be regularly surveyed by quality and performance indicators, and continuing education should provide current information.

 Purpose: We aimed to calculate preanalytical error rates in the Medical Microbiology Laboratory of our hospital by the six-sigma method and examine the effect of training on error rates by comparing performances of processes before and after training. Materials and methods: All samples evaluated between 2016-2021 were retrospectively examined. Rejected samples, blood culture contamination rate and urine culture contamination rate were evaluated via Laboratory Error Classification System. The staff obtaining laboratory samples were trained by means of live classes during 2017, 2018 and 2019, and with online classes during 2021. Error rates and sigma levels were calculated before and after training. Results: 685591 samples were accepted by our laboratory; 1175 0.2 were rejected. The most frequent cause of rejection 53.4 was hemolysis of sample. The sigma levels showed hemolysis of the sample as the most frequent cause of rejection, with a value of 4.7 good performance. Among other quality indicators, the rate of urinary culture contamination was 11.4, and the rate of blood culture contamination was 3.5. The total sigma level of urine culture contamination was 2.9 unacceptable performance, and the total blood culture contamination was 3.5 minimal performance. Error rates had generally decreased after training, while an increase in performance at the sigma level was detected at all three indicators. Conclusion: In order to minimize preanalytical errors in the medical laboratory, the preanalytical process should be regularly surveyed by quality and performance indicators, and continuing education should provide current information.

 Purpose: We aimed to calculate preanalytical error rates in the Medical Microbiology Laboratory of our hospital by the six-sigma method and examine the effect of training on error rates by comparing performances of processes before and after training. Materials and methods: All samples evaluated between 2016-2021 were retrospectively examined. Rejected samples, blood culture contamination rate and urine culture contamination rate were evaluated via Laboratory Error Classification System. The staff obtaining laboratory samples were trained by means of live classes during 2017, 2018 and 2019, and with online classes during 2021. Error rates and sigma levels were calculated before and after training. Results: 685591 samples were accepted by our laboratory; 1175 0.2 were rejected. The most frequent cause of rejection 53.4 was hemolysis of sample. The sigma levels showed hemolysis of the sample as the most frequent cause of rejection, with a value of 4.7 good performance. Among other quality indicators, the rate of urinary culture contamination was 11.4, and the rate of blood culture contamination was 3.5. The total sigma level of urine culture contamination was 2.9 unacceptable performance, and the total blood culture contamination was 3.5 minimal performance. Error rates had generally decreased after training, while an increase in performance at the sigma level was detected at all three indicators. Conclusion: In order to minimize preanalytical errors in the medical laboratory, the preanalytical process should be regularly surveyed by quality and performance indicators, and continuing education should provide current information.

 Amaç: Bu çalışmada, hastanemiz Tıbbi Mikrobiyoloji Laboratuvarında preanalitik hata oranlarının altı sigma yöntemi ile hesaplanması, eğitim öncesi ve sonrası süreç performansını karşılaştırarak eğitimin hata oranlarına etkisinin incelenmesi amaçlanmıştır. Gereç ve yöntem: 2016-2021 yılları arasında değerlendirilen tüm numuneler retrospektif olarak incelendi. Laboratuvar hata sınıflama sistemi LHSS üzerinden reddedilen numuneler, kan kültürü kontaminasyon oranı ve idrar kültürü kontaminasyon oranı gözden geçirildi. Numune alan personele 2017, 2018 ve 2019 yılında yüzyüze, 2021 yılında çevirimiçi eğitimler verildi, eğitim öncesi ve sonrası hata oranları ve sigma düzeyleri hesaplandı. Bulgular: Laboratuvarımıza 685591 numune kabul edilmiş, 1175i 0,2 reddedilmiştir. En sık ret nedeni hemolizli numunedir 53,4. Sigma düzeylerine bakıldığında en sık ret nedeni olan hemolizli numunede 4,7 iyi performans olarak saptanmıştır. Diğer kalite göstergelerinden idrar kültürü kontaminasyon oranı 11,4, kan kültürü kontaminasyon oranı 3,5 olarak bulunmuştur. İdrar kültürü kontaminasyonunun sigma düzeyine bakıldığında 2,9 kabul edilemez performans; kan kültürü kontaminasyonunun toplamda 3,5 minimum performans olduğu görülmüştür. Eğitim sonrası hata oranlarının genel olarak azaldığı görülmüş, sigma düzeyinde performans artışı her üç göstergede de tespit edilmiştir. Sonuç: Tıbbi laboratuvarlarda preanalitik hataları en aza indirebilmek için preanalitik süreç kalite ve performans göstergeleri ile düzenli olarak takip edilmeli, sürekli eğitimlerle de bilgilerin güncel kalması sağlanmalıdır.

Makale detayları

Evaluation of preanalytical error processes in the microbiology laboratory and effect of training on these processes

Yazarlar:

Nihan Ceken | Hülya Duran | Tuğba Kula Atik | Esin Avcı

Yayıncı:

Pamukkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 3, pp.648-659

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001329360

pISSN:

1308-0865

Microbiology laboratory

preanalytical error

trainingMikrobiyoloji laboratuvari

preanalitik hata

eğitim

Sayı

Makale detayları

Evaluation of preanalytical error processes in the microbiology laboratory and effect of training on these processes

Yazarlar: Nihan Ceken | Hülya Duran | Tuğba Kula Atik | Esin Avcı

Yayıncı: Pamukkale University

Ana Başlıklar: Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi: Pamukkale Tıp Dergisi Vol.18 Issue 3, pp.648-659

Belge Tipi: Makale

Yayın Tarihi: 2/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001329360

pISSN: 1308-0865

Microbiology laboratory

preanalytical error

trainingMikrobiyoloji laboratuvari

preanalitik hata

eğitim

Sayı

Yazarlar:

Nihan Ceken | Hülya Duran | Tuğba Kula Atik | Esin Avcı

Yayıncı:

Pamukkale University

Ana Başlıklar:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Dergi:

Pamukkale Tıp Dergisi Vol.18 Issue 3, pp.648-659

Belge Tipi:

Makale

Yayın Tarihi:

2/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001329360

pISSN:

1308-0865