Evaluation of orange peel, an industrial waste, for the production of Aspergillus sojae polygalacturonase considering both morphology and rheology effects

Nihan GÖĞÜŞ; Bengi Hakgüder TAZE; Hande DEMİR; Canan TARI; Sevcan ÜNLÜTÜRK; Marcelo Fernandez LAHORE

doi:https://doi.org/10.3906/biy-1308-47

Makale detayları

Evaluation of orange peel, an industrial waste, for the production of Aspergillus sojae polygalacturonase considering both morphology and rheology effects

Yazarlar:

Nihan GÖĞÜŞ | Bengi Hakgüder TAZE | Hande DEMİR | Canan TARI | Sevcan ÜNLÜTÜRK | Marcelo Fernandez LAHORE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.537-548

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812176

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1308-47

Anahtar kelimeler:

Özet:

 

Orange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by Aspergillus sojae using statistical approaches. Effect of pH, another significant parameter affecting the rheology and morphology of the strain, was investigated in the serial bioreactor system using the optimized medium composition. Highest PG enzyme yield and productivity together with the maximum PG enzyme production (93.48 U/mL) were obtained under uncontrolled pH conditions. Under these conditions, morphologically, pellet sizes exhibited a normal distribution ranging between 0.5-1.0 mm and 1.0-1.5 mm, and rheological measurements revealed that fermentation broths showed non-Newtonian flow. The low pH trend observed during the course of the fermentation was another important positive outcome for industrial fermentations, prone to contamination problems.

 

Orange peel is an agroindustrial waste rich in pectin and known to be an inducer for pectinase production. The use of this low-cost substrate for the production of an industrially important enzyme, polygalacturonase (PG), can be an alternative way to turn this waste into a value-added product, contributing to the reduction of environmental waste disposal problems. Enzyme productions by fungal microorganisms are affected by environmental and nutritional factors, demanding the determination of optimum conditions for maximum enzyme production with the desired fungal morphology and broth rheology. Therefore, complex and additional carbon sources were optimized with respect to PG production by Aspergillus sojae using statistical approaches. Effect of pH, another significant parameter affecting the rheology and morphology of the strain, was investigated in the serial bioreactor system using the optimized medium composition. Highest PG enzyme yield and productivity together with the maximum PG enzyme production (93.48 U/mL) were obtained under uncontrolled pH conditions. Under these conditions, morphologically, pellet sizes exhibited a normal distribution ranging between 0.5-1.0 mm and 1.0-1.5 mm, and rheological measurements revealed that fermentation broths showed non-Newtonian flow. The low pH trend observed during the course of the fermentation was another important positive outcome for industrial fermentations, prone to contamination problems.

Makale detayları

Evaluation of orange peel, an industrial waste, for the production of Aspergillus sojae polygalacturonase considering both morphology and rheology effects

Yazarlar:

Nihan GÖĞÜŞ | Bengi Hakgüder TAZE | Hande DEMİR | Canan TARI | Sevcan ÜNLÜTÜRK | Marcelo Fernandez LAHORE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.537-548

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812176

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1308-47

Polygalacturonase

Aspergillus sojae

orange peel

broth rheology

medium pH

fungal morphology

agroindustrial waste

Sayı

Makale detayları

Yazarlar: Nihan GÖĞÜŞ | Bengi Hakgüder TAZE | Hande DEMİR | Canan TARI | Sevcan ÜNLÜTÜRK | Marcelo Fernandez LAHORE

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.537-548

Belge Tipi: Makale

Yayın Tarihi: 3/2014

Belge Dili: İngilizce

Document ID (Org No.): 2000812176

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1308-47

Sayı

Yazarlar:

Nihan GÖĞÜŞ | Bengi Hakgüder TAZE | Hande DEMİR | Canan TARI | Sevcan ÜNLÜTÜRK | Marcelo Fernandez LAHORE

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2014 Vol.38 Issue 4, pp.537-548

Belge Tipi:

Makale

Yayın Tarihi:

3/2014

Belge Dili:

İngilizce

Document ID (Org No.):

2000812176

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1308-47