Ocimum basilicum-Assisted Gold Nanoparticle Formation: Structural Features and Antimicrobial Performance

Muhammed Said Özonay; Cumali Keskin; Ayşe Baran; Özgür Topgider; Şeyma Asyalı

Makale detayları

Ocimum basilicum-Assisted Gold Nanoparticle Formation: Structural Features and Antimicrobial Performance

Yazarlar:

Muhammed Said Özonay | Cumali Keskin | Ayşe Baran | Özgür Topgider | Şeyma Asyalı

Yayıncı:

Ordu University

Ana Başlıklar:

Clinical Chemistry | Clinical Microbiology

Dergi:

Middle Black Sea Journal of Health Science Vol.11 Issue 3, pp.190-211

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357759

pISSN:

2149-7796

Anahtar kelimeler:

Özet:

 Objective: Plant-based biosynthesis of gold nanoparticles AuNPs can reduce the biological consequences of NPs released into the environment by replacing toxic substances. The Lamiaceae family includes the perennial herb Ocimum basilicum OB, which is typically found in temperate climates. This study aimed to synthesize biogenic metallic nanoparticles ecologically to provide agricultural, medical, and pharmacological options from OB leaf aqueous extract. Method: Tetrachloroauric acid HAuCl4.3H2O solution and OB extract were the chemicals, stabilizers, or surfactants used in the biological synthesis process to create gold nanoparticles OB-AuNPs. Data from UV-Vis, SEM-EDX, XRD, FT-IR, TGA-DTA, AFM, and zeta potential were used to characterize the produced AuNPs. The minimum inhibitory concentration MIC method was used to test the antimicrobial activity. Results: UV-Vis data revealed that the produced gold nanoparticles produced a notable plasmon resonance at around 532 nm. SEM-EDX examination verified that the synthesized nanomaterials had a spherical crystal structure. The reduction of Au3 ions using the OB extract resulted in the production of crystalline AuNPs, as demonstrated by the high-resolution and robust XRD pattern. Analysis of XRD and SEM-EDX data revealed that gold, oxygen, and carbon made up the majority of OB-AuNPs element content. The surface charges of AuNPs were determined to be  17 mV. Conclusion: The negative zeta potential indicates good stability of the nanoparticles in suspension, suggesting that they are less likely to aggregate over time. It was found that, in comparison to conventional antibiotics, OB-AuNPs produced utilizing an extract from the leaves of the OB had more potent inhibitory activity on the growth of yeast and pathogenic gram bacteria. Overall, these findings support the potential application of AuNPs in various fields, including biomedical and environmental technologies.

 Objective: Plant-based biosynthesis of gold nanoparticles AuNPs can reduce the biological consequences of NPs released into the environment by replacing toxic substances. The Lamiaceae family includes the perennial herb Ocimum basilicum OB, which is typically found in temperate climates. This study aimed to synthesize biogenic metallic nanoparticles ecologically to provide agricultural, medical, and pharmacological options from OB leaf aqueous extract. Method: Tetrachloroauric acid HAuCl4.3H2O solution and OB extract were the chemicals, stabilizers, or surfactants used in the biological synthesis process to create gold nanoparticles OB-AuNPs. Data from UV-Vis, SEM-EDX, XRD, FT-IR, TGA-DTA, AFM, and zeta potential were used to characterize the produced AuNPs. The minimum inhibitory concentration MIC method was used to test the antimicrobial activity. Results: UV-Vis data revealed that the produced gold nanoparticles produced a notable plasmon resonance at around 532 nm. SEM-EDX examination verified that the synthesized nanomaterials had a spherical crystal structure. The reduction of Au3 ions using the OB extract resulted in the production of crystalline AuNPs, as demonstrated by the high-resolution and robust XRD pattern. Analysis of XRD and SEM-EDX data revealed that gold, oxygen, and carbon made up the majority of OB-AuNPs element content. The surface charges of AuNPs were determined to be  17 mV. Conclusion: The negative zeta potential indicates good stability of the nanoparticles in suspension, suggesting that they are less likely to aggregate over time. It was found that, in comparison to conventional antibiotics, OB-AuNPs produced utilizing an extract from the leaves of the OB had more potent inhibitory activity on the growth of yeast and pathogenic gram bacteria. Overall, these findings support the potential application of AuNPs in various fields, including biomedical and environmental technologies.

 Objective: Plant-based biosynthesis of gold nanoparticles AuNPs can reduce the biological consequences of NPs released into the environment by replacing toxic substances. The Lamiaceae family includes the perennial herb Ocimum basilicum OB, which is typically found in temperate climates. This study aimed to synthesize biogenic metallic nanoparticles ecologically to provide agricultural, medical, and pharmacological options from OB leaf aqueous extract. Method: Tetrachloroauric acid HAuCl4.3H2O solution and OB extract were the chemicals, stabilizers, or surfactants used in the biological synthesis process to create gold nanoparticles OB-AuNPs. Data from UV-Vis, SEM-EDX, XRD, FT-IR, TGA-DTA, AFM, and zeta potential were used to characterize the produced AuNPs. The minimum inhibitory concentration MIC method was used to test the antimicrobial activity. Results: UV-Vis data revealed that the produced gold nanoparticles produced a notable plasmon resonance at around 532 nm. SEM-EDX examination verified that the synthesized nanomaterials had a spherical crystal structure. The reduction of Au3 ions using the OB extract resulted in the production of crystalline AuNPs, as demonstrated by the high-resolution and robust XRD pattern. Analysis of XRD and SEM-EDX data revealed that gold, oxygen, and carbon made up the majority of OB-AuNPs element content. The surface charges of AuNPs were determined to be  17 mV. Conclusion: The negative zeta potential indicates good stability of the nanoparticles in suspension, suggesting that they are less likely to aggregate over time. It was found that, in comparison to conventional antibiotics, OB-AuNPs produced utilizing an extract from the leaves of the OB had more potent inhibitory activity on the growth of yeast and pathogenic gram bacteria. Overall, these findings support the potential application of AuNPs in various fields, including biomedical and environmental technologies.

 Objective: Plant-based biosynthesis of gold nanoparticles AuNPs can reduce the biological consequences of NPs released into the environment by replacing toxic substances. The Lamiaceae family includes the perennial herb Ocimum basilicum OB, which is typically found in temperate climates. This study aimed to synthesize biogenic metallic nanoparticles ecologically to provide agricultural, medical, and pharmacological options from OB leaf aqueous extract. Method: Tetrachloroauric acid HAuCl4.3H2O solution and OB extract were the chemicals, stabilizers, or surfactants used in the biological synthesis process to create gold nanoparticles OB-AuNPs. Data from UV-Vis, SEM-EDX, XRD, FT-IR, TGA-DTA, AFM, and zeta potential were used to characterize the produced AuNPs. The minimum inhibitory concentration MIC method was used to test the antimicrobial activity. Results: UV-Vis data revealed that the produced gold nanoparticles produced a notable plasmon resonance at around 532 nm. SEM-EDX examination verified that the synthesized nanomaterials had a spherical crystal structure. The reduction of Au3 ions using the OB extract resulted in the production of crystalline AuNPs, as demonstrated by the high-resolution and robust XRD pattern. Analysis of XRD and SEM-EDX data revealed that gold, oxygen, and carbon made up the majority of OB-AuNPs element content. The surface charges of AuNPs were determined to be  17 mV. Conclusion: The negative zeta potential indicates good stability of the nanoparticles in suspension, suggesting that they are less likely to aggregate over time. It was found that, in comparison to conventional antibiotics, OB-AuNPs produced utilizing an extract from the leaves of the OB had more potent inhibitory activity on the growth of yeast and pathogenic gram bacteria. Overall, these findings support the potential application of AuNPs in various fields, including biomedical and environmental technologies.

Makale detayları

Ocimum basilicum-Assisted Gold Nanoparticle Formation: Structural Features and Antimicrobial Performance

Yazarlar:

Muhammed Said Özonay | Cumali Keskin | Ayşe Baran | Özgür Topgider | Şeyma Asyalı

Yayıncı:

Ordu University

Ana Başlıklar:

Clinical Chemistry | Clinical Microbiology

Dergi:

Middle Black Sea Journal of Health Science Vol.11 Issue 3, pp.190-211

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357759

pISSN:

2149-7796

Ocimum basilicum (Basil extract)

antimicrobial activity

characterization

plant-mediated synthesis

Gold nanoparticles (AuNPs)

Sayı

Makale detayları

Ocimum basilicum-Assisted Gold Nanoparticle Formation: Structural Features and Antimicrobial Performance

Yazarlar: Muhammed Said Özonay | Cumali Keskin | Ayşe Baran | Özgür Topgider | Şeyma Asyalı

Yayıncı: Ordu University

Ana Başlıklar: Clinical Chemistry | Clinical Microbiology

Dergi: Middle Black Sea Journal of Health Science Vol.11 Issue 3, pp.190-211

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357759

pISSN: 2149-7796

Ocimum basilicum (Basil extract)

antimicrobial activity

characterization

plant-mediated synthesis

Gold nanoparticles (AuNPs)

Sayı

Yazarlar:

Muhammed Said Özonay | Cumali Keskin | Ayşe Baran | Özgür Topgider | Şeyma Asyalı

Yayıncı:

Ordu University

Ana Başlıklar:

Clinical Chemistry | Clinical Microbiology

Dergi:

Middle Black Sea Journal of Health Science Vol.11 Issue 3, pp.190-211

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357759

pISSN:

2149-7796