Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Putra Santoso

Makale detayları

Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Yazarlar:

Putra Santoso

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 5, pp.1400-1408

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347571

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Yam bean Pachyrhizus erosus L. is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber YB is also capable of counteracting the deleterious effects of a high-fat diet HFD on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 GLP-1 expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet ND, HFD, and HFD supplemented with 10 of YB HFD  YB. After eight weeks, mRNA expression of GLP-1, malondialdehyde MDA levels, catalase CAT activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

 Yam bean Pachyrhizus erosus L. is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber YB is also capable of counteracting the deleterious effects of a high-fat diet HFD on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 GLP-1 expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet ND, HFD, and HFD supplemented with 10 of YB HFD  YB. After eight weeks, mRNA expression of GLP-1, malondialdehyde MDA levels, catalase CAT activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

 Yam bean Pachyrhizus erosus L. is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber YB is also capable of counteracting the deleterious effects of a high-fat diet HFD on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 GLP-1 expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet ND, HFD, and HFD supplemented with 10 of YB HFD  YB. After eight weeks, mRNA expression of GLP-1, malondialdehyde MDA levels, catalase CAT activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

 Yam bean Pachyrhizus erosus L. is a prominent medicinal plant exerting various health benefits. However, it remains unknown whether yam bean fiber YB is also capable of counteracting the deleterious effects of a high-fat diet HFD on the small intestine. This present study aimed to investigate the effect of YB supplementation on the glucagon-like peptide 1 GLP-1 expression, oxidative stress, and histopathological alterations of the small intestine in mice fed an HFD. Twenty-seven mice were assigned equally into three different diet treatment groups: normal diet ND, HFD, and HFD supplemented with 10 of YB HFD  YB. After eight weeks, mRNA expression of GLP-1, malondialdehyde MDA levels, catalase CAT activity in the small intestinal tissue were determined, and the anatomical and histopathological alterations of the intestine were investigated. The results demonstrated that YB supplementation was effective in precluding HFD-indued reduction of GLP-1 mRNA expression in the small intestine. YBF also prevented MDA elevation while ameliorating CAT activity. Furthermore, YBF exerted an ameliorative effect against HFD-induced morphological and histological alterations of the small intestine, particularly in intestinal length, index, and the number of inflammatory cells in the mucosal layer. This study revealed that YB supplementation could effectively prevent the impairment of GLP-1 expression and oxidative stress while ameliorating the histopathological alterations of the small intestine caused by HFD. Hence, YB could be formulated as a supplement to improve the gastrointestinal structure and functions against HFD.

Makale detayları

Ameliorative effect of yam bean (Pachyrhizus erosus L.) fiber on glucagon-like peptide 1 expression, oxidative stress and histopathology of the small ıntestine in mice fed high-fat diet

Yazarlar:

Putra Santoso

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 5, pp.1400-1408

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347571

pISSN:

2630-6344

catalase activity

GLP-1

inflammatory cells

malondialdehyde

oxidative stress

small intestine

yam bean

Sayı

Makale detayları

Yazarlar: Putra Santoso

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.28 Issue 5, pp.1400-1408

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347571

pISSN: 2630-6344

Sayı

Yazarlar:

Putra Santoso

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 5, pp.1400-1408

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347571

pISSN:

2630-6344