Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System

Pelin Kolçak Yaşlı; Seda Kulabaş; Evren Doruk Engin

Makale detayları

Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System

Yazarlar:

Pelin Kolçak Yaşlı | Seda Kulabaş | Evren Doruk Engin

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Bioprocessing | Bioproduction and Bioproducts | Industrial Biotechnology (Other)

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.571-586

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328948

pISSN:

2757-5195

Anahtar kelimeler:

Özet:

 Chinese hamster ovary CHO mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as i the integration region in the genome, ii the size of the target cassette, and iii long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -- cells and the Sleeping Beauty SB transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mgL in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mgL. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.

 Chinese hamster ovary CHO mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as i the integration region in the genome, ii the size of the target cassette, and iii long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -- cells and the Sleeping Beauty SB transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mgL in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mgL. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.

 Chinese hamster ovary CHO mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as i the integration region in the genome, ii the size of the target cassette, and iii long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -- cells and the Sleeping Beauty SB transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mgL in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mgL. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.

 Chinese hamster ovary CHO mammalian cell lines are widely used as cell platforms in biopharmaceutical productions. Different transfection systems are employed for the integration of the target gene cassette into the cell genome and have limitations, such as i the integration region in the genome, ii the size of the target cassette, and iii long selection periods for stable expression. Transposon systems can be utilized to overcome the limitations mentioned in the efficient production of commercially significant recombinant proteins. This study aims to demonstrate the differences in production potential and selection periods by using a specially designed vector system for random genome integration in CHODG44 DHFR -- cells and the Sleeping Beauty SB transposon system. In this context, the optimal transfer ratio between the donor and the helper plasmid was determined for the most efficient co-transfection in the SB transposon system. According to the results, the pools obtained using the SB transposon system had titers ranging from 1300 to 2600 mgL in 13-day fed-batch studies, while the pool obtained using the random transfer system had a titer of 0.056 mgL. Additionally, stable cell pools obtained using the transposon system underwent selection in a short period of 52 days, compared to over 100 days for the pool obtained through random transfer. Considering all these results together, it is demonstrated that stable CHO pools obtained using the optimal SB transposon system can achieve high-efficiency monoclonal antibody production in a short period, making it an optimal production platform in the biopharmaceutical field.

Makale detayları

Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System

Yazarlar:

Pelin Kolçak Yaşlı | Seda Kulabaş | Evren Doruk Engin

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Bioprocessing | Bioproduction and Bioproducts | Industrial Biotechnology (Other)

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.571-586

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328948

pISSN:

2757-5195

Sleeping Beauty transposon

gene integration

expression level

CHO cells

industrial production

Sayı

Makale detayları

Evaluation of Recombinant Antibody Production Efficiency in CHO Cells with Sleeping Beauty Transposon Vector System

Yazarlar: Pelin Kolçak Yaşlı | Seda Kulabaş | Evren Doruk Engin

Yayıncı: Çanakkale Onsekiz Mart University

Ana Başlıklar: Bioprocessing | Bioproduction and Bioproducts | Industrial Biotechnology (Other)

Dergi: Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.571-586

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328948

pISSN: 2757-5195

Sleeping Beauty transposon

gene integration

expression level

CHO cells

industrial production

Sayı

Yazarlar:

Pelin Kolçak Yaşlı | Seda Kulabaş | Evren Doruk Engin

Yayıncı:

Çanakkale Onsekiz Mart University

Ana Başlıklar:

Bioprocessing | Bioproduction and Bioproducts | Industrial Biotechnology (Other)

Dergi:

Journal of Advanced Research in Natural and Applied Sciences Vol.10 Issue 3, pp.571-586

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328948

pISSN:

2757-5195