Non enzymatic isolation of adipose tissue and stromal vascular fraction  derived cells

Ece ÇERÇİ; Hatice ERDOST

doi:https://doi.org/10.33988/auvfd.660139

Makale detayları

Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Yazarlar:

Ece ÇERÇİ | Hatice ERDOST

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 3, pp.295-301

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849489

DOI:

https://doi.org/10.33988/auvfd.660139

Özet:

 

The therapeutic potential of the adipose tissue is also supported by a source of mesenchymal stem cells (MSCs), endothelial progenitor cells, mast cells, T-  lymphocyte, B lymphocyte, and adipose-resident macrophages with repairing and regenerative ability. The purpose of this study was to compare adipose tissue derived mesenchymal stem cells (ADSCs) and stromal vascular fraction cells (SVF cells) in terms of the usage of non-enzymatic isolation, detection of cell adhesion, fibroblastoid cell formation, properties of cell population, cell culture duration until 3rd passages under in-vitro culture condition. We used 3 months old, 4 male Sprague Dawley rats (mean of live weight about 250 g) to examined the variation of the cell population, cell properties, phases of cellular formation, cell culturing time, subculturing duration, differences in confluency between SVF cells and ADSCs. In in-vitro expansion, ADSCs displayed higher rate of adhesiveness, homogenous cell population, faster proliferation and formation of fibroblast like cells compared to SVF cells.  As a result, we showed that ADSCs have better adhesive ability, higher proliferative capacity in all of the 3 passages and require shorter time to reach confluency compared with SVF cells in vitro. These findings may contribute to future studies that deal with isolation and selection of stem cells from various tissues, as well as design clinical trials based on ADSCs and SVF cells.

Makale detayları

Non enzymatic isolation of adipose tissue and stromal vascular fraction derived cells

Yazarlar: Ece ÇERÇİ | Hatice ERDOST

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 3, pp.295-301

Belge Tipi: Makale

Yayın Tarihi: 6/2020

Belge Dili: İngilizce

Document ID (Org No.): 2000849489

DOI: https://doi.org/10.33988/auvfd.660139

Sayı

Yazarlar:

Ece ÇERÇİ | Hatice ERDOST

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2020 Vol.67 Issue 3, pp.295-301

Belge Tipi:

Makale

Yayın Tarihi:

6/2020

Belge Dili:

İngilizce

Document ID (Org No.):

2000849489

DOI:

https://doi.org/10.33988/auvfd.660139