Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro

Hatem ATALLA; Mithat EVECEN; Serhat PABUCCUOĞLU; Sema BİRLER

Makale detayları

Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro

Yazarlar:

Hatem ATALLA | Mithat EVECEN | Serhat PABUCCUOĞLU | Sema BİRLER

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.83-87

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000849710

Özet:

 

The effects of two vitrification protocols on the survival of sheep blastocysts were examined in embryos producedin vitro with sheep oviduct epithelial cells co-culture (CC) or without co-culture (C). Oocytes collected from slaughtered ewes werematured for 24 h, fertilized with fresh ram semen for 20 h and cultured in SOF medium for up to 9 days in vitro. For vitrification,blastocyst stage embryos were assigned to two equilibration groups (20% ethylen glycol (EG) or 10% glycerol (G) for the firstequilibration), and as the second equilibration they were kept in 20% ethylen glycol plus 10% glycerol for 5 minutes. After 30 sec invitrification solution (25% ethylen glycol + 25% glycerol), they were immediately immersed into liquid nitrogen. After thawingprocedure, embryos were transferred into 0.25 M sucrose for 5 min, washed in Hepes buffered synthetic oviduct fluid (HSOF) andcultured in SOF medium for 24 h. Cleavage rates were 75.2% in C and 74.2% in CC groups, and blastocyst rates were 14.4% in C and17.1% in CC groups. After in vitro culture of vitrified-thawed blastocysts, survival rates were 62.1, 38.4, 30.2, and 39.3% in C-EG,CC-EG, C-G and CC-G groups, respectively. This study shows that vitrification of sheep embryos using ethylene glycol instead ofglycerol as a first equilibration cryoprotectant could give reasonable survival rates and that co-culture of embryos with sheep oviductepithelial cell has no beneficial effect on vitrification of embryos.  .

Makale detayları

Effects of equilibration media and co-culture on vitrification of sheep blastocysts derived in vitro

Yazarlar: Hatem ATALLA | Mithat EVECEN | Serhat PABUCCUOĞLU | Sema BİRLER

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.83-87

Belge Tipi: Makale

Yayın Tarihi: 12/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000849710

Sayı

Yazarlar:

Hatem ATALLA | Mithat EVECEN | Serhat PABUCCUOĞLU | Sema BİRLER

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 1, pp.83-87

Belge Tipi:

Makale

Yayın Tarihi:

12/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000849710