Comparison of Antibiotic Susceptibility of Carbapanem-Resistant Enterobactericeae Species  with Two Different Panels (Phoenix BD)

Hacer Uğurlu; Burak Küçük; Filiz Orak; Murat Aral

Makale detayları

Comparison of Antibiotic Susceptibility of Carbapanem-Resistant Enterobactericeae Species with Two Different Panels (Phoenix BD)

Yazarlar:

Hacer Uğurlu | Burak Küçük | Filiz Orak | Murat Aral

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001285368

pISSN:

1303-6610

Anahtar kelimeler:

Özet:

 Objective: The aim of this study is to compare the antibiotic susceptibility of carbapanem-resistant Enterobactericeae strains isolated in the microbiology laboratory of our hospital with two different panels and to evaluate the performance of the CPO test. Material and Methods: The samples sent to the microbiology laboratory of our hospital between 01.01.2020-31.12.2020 were evaluated. Specimens with growth were loaded into the BD Phoenix 100 automated system BD Phoenix NMIC 433 panel, and 50 isolates of Enterobacteriaceae that resulted in carbapenem resistant were studied with the BD Phoenix NMIC 505 CPO panel. 50 carbapenem-resistant Enterobacteriaceae isolates were classified according to Ambler class A, B, and D by the CPO panel. Results: Of the isolates, 28 56 were isolated from male patients and 22 44 from female patients. Of the carbapenem-resistant Enterobacteriaceae isolates, 43 86 were identified as Klebsiella pneumoniae and 4 8 were Escherichia coli. When the evaluation of antibiotic susceptibility rates of the CPO panel and NMIC 433 panel of carbapenem-resistant Enterobacteriaceae isolates were examined; Antibiotic susceptibility results of 50 Enterobacte- riaceae isolates studied in both panels were found to be the same. When the antibiotics that can be studied in the CPO panel were examined, it was observed that the most effective antibiotic was ceftazidime-avibactam. When the results were examined, it was observed that 46 92 isolates were susceptible to ceftazidime-avibactam. The number of bacteria producing class B carbapenemase was 4 8 and the number of bacteria producing class D carbapenemase was 42 84. Conclusions: It should be noted that ceftazidime-avibactam can be used as a treatment option in carbapenem-resistant species. Further work on the CPO panel will help identify the contribution of the CPO panel to the routine workflow.

 Objective: The aim of this study is to compare the antibiotic susceptibility of carbapanem-resistant Enterobactericeae strains isolated in the microbiology laboratory of our hospital with two different panels and to evaluate the performance of the CPO test. Material and Methods: The samples sent to the microbiology laboratory of our hospital between 01.01.2020-31.12.2020 were evaluated. Specimens with growth were loaded into the BD Phoenix 100 automated system BD Phoenix NMIC 433 panel, and 50 isolates of Enterobacteriaceae that resulted in carbapenem resistant were studied with the BD Phoenix NMIC 505 CPO panel. 50 carbapenem-resistant Enterobacteriaceae isolates were classified according to Ambler class A, B, and D by the CPO panel. Results: Of the isolates, 28 56 were isolated from male patients and 22 44 from female patients. Of the carbapenem-resistant Enterobacteriaceae isolates, 43 86 were identified as Klebsiella pneumoniae and 4 8 were Escherichia coli. When the evaluation of antibiotic susceptibility rates of the CPO panel and NMIC 433 panel of carbapenem-resistant Enterobacteriaceae isolates were examined; Antibiotic susceptibility results of 50 Enterobacte- riaceae isolates studied in both panels were found to be the same. When the antibiotics that can be studied in the CPO panel were examined, it was observed that the most effective antibiotic was ceftazidime-avibactam. When the results were examined, it was observed that 46 92 isolates were susceptible to ceftazidime-avibactam. The number of bacteria producing class B carbapenemase was 4 8 and the number of bacteria producing class D carbapenemase was 42 84. Conclusions: It should be noted that ceftazidime-avibactam can be used as a treatment option in carbapenem-resistant species. Further work on the CPO panel will help identify the contribution of the CPO panel to the routine workflow.

 Objective: The aim of this study is to compare the antibiotic susceptibility of carbapanem-resistant Enterobactericeae strains isolated in the microbiology laboratory of our hospital with two different panels and to evaluate the performance of the CPO test. Material and Methods: The samples sent to the microbiology laboratory of our hospital between 01.01.2020-31.12.2020 were evaluated. Specimens with growth were loaded into the BD Phoenix 100 automated system BD Phoenix NMIC 433 panel, and 50 isolates of Enterobacteriaceae that resulted in carbapenem resistant were studied with the BD Phoenix NMIC 505 CPO panel. 50 carbapenem-resistant Enterobacteriaceae isolates were classified according to Ambler class A, B, and D by the CPO panel. Results: Of the isolates, 28 56 were isolated from male patients and 22 44 from female patients. Of the carbapenem-resistant Enterobacteriaceae isolates, 43 86 were identified as Klebsiella pneumoniae and 4 8 were Escherichia coli. When the evaluation of antibiotic susceptibility rates of the CPO panel and NMIC 433 panel of carbapenem-resistant Enterobacteriaceae isolates were examined; Antibiotic susceptibility results of 50 Enterobacte- riaceae isolates studied in both panels were found to be the same. When the antibiotics that can be studied in the CPO panel were examined, it was observed that the most effective antibiotic was ceftazidime-avibactam. When the results were examined, it was observed that 46 92 isolates were susceptible to ceftazidime-avibactam. The number of bacteria producing class B carbapenemase was 4 8 and the number of bacteria producing class D carbapenemase was 42 84. Conclusions: It should be noted that ceftazidime-avibactam can be used as a treatment option in carbapenem-resistant species. Further work on the CPO panel will help identify the contribution of the CPO panel to the routine workflow.

 Amaç: Bu çalışmanın amacı; hastanemiz mikrobiyoloji laboratuvarında izole edilen karbapenem dirençli Enterobactericeae türlerinin antibiyotik duyarlılıklarının iki farklı panelle karşılaştırılması ve CPO testinin perfomansının değerlendirilmesidir. Gereç ve Yöntemler: 01.01.2020-31.12.2020 tarihleri arasında hastanemiz mikrobiyoloji laboratuvarına gönderilen örnekler değerlendirilmiştir. Üremesi olan örnekler BD Phoenix 100 otomatize sistem BD Phoenix NMIC 433 paneline yüklenmiş olup, karbapenem dirençli olarak sonuçlanan 50 Enterobacteriaceae izolatı BD Phoenix NMIC 505 CPO paneliyle çalışılmıştır. Karbapenem dirençli 50 Enterobacteriaceae izolatı CPO paneliyle Ambler sınıfına göre A, B ve Dye göre sınıflandırılmıştır. Bulgular: İzolatların 28i 56 erkek hastalardan, 22si 44 kadın hastalardan izole edilmiştir. Karbapenem dirençli Enterobacteriaceae izolatlarının 43ü 86 Klebsiella pneumoniae, 4ü 8 Escherichia coli olarak saptanmıştır. Karbapenem dirençli Enterobacteriaceae izolatlarının CPO paneli ve NMIC 433 panelinin antibiyotik duyarlılık oranlarının değerlendirmesi incelendiğinde; iki panelde de çalışılan 50 Enterobacteriaceae izolatının antibiyotik duyarlılık sonuçlarının aynı olduğu saptanmıştır. CPO panelinde çalışılabilen antibiyotikler incelendiğinde en etkili antibiyotiğin seftazidim-avibaktam olduğu gözlenmiştir. Sonuçlar incelendiğinde seftazidim-avibaktama 46 92 izolatın duyarlı olduğu gözlemlenmiştir. Sınıf B karbapenemaz üreten bakteri sayısı 4 8, sınıf D karbapenemaz üreten bakteri sayısı 42 84 olarak belirlenmiştir. Sonuçlar: Seftazidim-avibaktamın karbapenem dirençli türlerde tedavi seçeneği olarak kullanılabileceği unutulmamalıdır. CPO paneli ile ilgili daha fazla çalışma yapılması, CPO panelinin rutin iş akışına katkısının belirlenmesine fayda sağlayacaktır.

Makale detayları

Comparison of Antibiotic Susceptibility of Carbapanem-Resistant Enterobactericeae Species with Two Different Panels (Phoenix BD)

Yazarlar:

Hacer Uğurlu | Burak Küçük | Filiz Orak | Murat Aral

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001285368

pISSN:

1303-6610

Antimicrobial susceptibility test

carbapenemase

ceftazidime avibactam

CPO panel

Enterobactericeae.

Antimikrobiyal duyarlılık testi

CPO panel

Enterobactericeae

karbapenemaz

seftazidim avibaktam.

Sayı

Makale detayları

Yazarlar: Hacer Uğurlu | Burak Küçük | Filiz Orak | Murat Aral

Yayıncı: Kahramanmaras Sutcu Imam University

Ana Başlıklar: Sağlık Kurumları Yönetimi

Dergi: Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 1, pp.22-27

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001285368

pISSN: 1303-6610

Sayı

Yazarlar:

Hacer Uğurlu | Burak Küçük | Filiz Orak | Murat Aral

Yayıncı:

Kahramanmaras Sutcu Imam University

Ana Başlıklar:

Sağlık Kurumları Yönetimi

Dergi:

Kahramanmaraş Sütçü İmam Üniversitesi Tıp Fakültesi Dergisi Vol.18 Issue 1, pp.22-27

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001285368

pISSN:

1303-6610