Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

KEE HWA BAE

Makale detayları

Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

Yazarlar:

KEE HWA BAE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.732-739

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811852

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Abstract: Bupleurum latissimum Nakai is a critically important endangered plant belonging to the family Apiaceae. Seed germination was promoted by soaking in the dormancy breaker gibberellic acid (GA3) (50% germination as compared with 4% of the control). An optimal temperature for germination of seed previously soaked in GA3 solution was determined by incubation at various temperatures. Seed germination of 31.8% was observed at 25 °C. These results indicate that the seeds of B. latissimum are difficult to germinate, even when treated with GA3. The greatest callus induction (94.8%) was observed in root explants of seedlings grown on MS medium containing a specific concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) (1.0 mg L-1) and N6-benzyladenine (BA) (3.0 mg L-1). Induction of somatic embryo was observed in 78.5% of the root segment cultured on MS medium containing 3.0 mg L-1 2,4-D alone. The highest shoot induction rate was obtained in MS medium containing 30 g L-1 sucrose (number of shoots, 6.8; average length of shoot, 8.8 cm). After acclimation in artificial soil (1:1:1 mixture of peat moss, perlite, and sand) and transfer to the greenhouse, 98% of plantlets survived over 2 months. This in vitro propagation protocol will be very useful for the conservation of this critically endangered plant.

 

Abstract: Bupleurum latissimum Nakai is a critically important endangered plant belonging to the family Apiaceae. Seed germination was promoted by soaking in the dormancy breaker gibberellic acid (GA3) (50% germination as compared with 4% of the control). An optimal temperature for germination of seed previously soaked in GA3 solution was determined by incubation at various temperatures. Seed germination of 31.8% was observed at 25 °C. These results indicate that the seeds of B. latissimum are difficult to germinate, even when treated with GA3. The greatest callus induction (94.8%) was observed in root explants of seedlings grown on MS medium containing a specific concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) (1.0 mg L-1) and N6-benzyladenine (BA) (3.0 mg L-1). Induction of somatic embryo was observed in 78.5% of the root segment cultured on MS medium containing 3.0 mg L-1 2,4-D alone. The highest shoot induction rate was obtained in MS medium containing 30 g L-1 sucrose (number of shoots, 6.8; average length of shoot, 8.8 cm). After acclimation in artificial soil (1:1:1 mixture of peat moss, perlite, and sand) and transfer to the greenhouse, 98% of plantlets survived over 2 months. This in vitro propagation protocol will be very useful for the conservation of this critically endangered plant.

Makale detayları

Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

Yazarlar:

KEE HWA BAE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.732-739

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811852

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Bupleurum latissimum Nakai

callus

endangered

endemic

GA3

regeneration

Sayı

Makale detayları

Improvement of seed germination and in vitro propagation of Bupleurum latissimum Nakai through embryogenesis

Yazarlar: KEE HWA BAE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.732-739

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811852

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Bupleurum latissimum Nakai

callus

endangered

endemic

GA3

regeneration

Sayı

Yazarlar:

KEE HWA BAE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.732-739

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811852

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152