Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

Cem Onal

Makale detayları

Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

Yazarlar:

Cem Onal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.765-773

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348000

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 A sensitive method is presented for the determination of the ticagrelor TCG in human plasma and pharmaceutical preparations using 5-dimethylaminonaphthalene-1-sulfonyl chloride dansyl chloride. Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate pH 10 to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0 200.0 ng mL1, with a coefficient of determination of 0.9999 with a limit of detection LOD of 0.14 ng mL1 and the limit of quantitation LOQ of 0.46 ng mL1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.

 A sensitive method is presented for the determination of the ticagrelor TCG in human plasma and pharmaceutical preparations using 5-dimethylaminonaphthalene-1-sulfonyl chloride dansyl chloride. Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate pH 10 to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0 200.0 ng mL1, with a coefficient of determination of 0.9999 with a limit of detection LOD of 0.14 ng mL1 and the limit of quantitation LOQ of 0.46 ng mL1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.

 A sensitive method is presented for the determination of the ticagrelor TCG in human plasma and pharmaceutical preparations using 5-dimethylaminonaphthalene-1-sulfonyl chloride dansyl chloride. Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate pH 10 to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0 200.0 ng mL1, with a coefficient of determination of 0.9999 with a limit of detection LOD of 0.14 ng mL1 and the limit of quantitation LOQ of 0.46 ng mL1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.

 A sensitive method is presented for the determination of the ticagrelor TCG in human plasma and pharmaceutical preparations using 5-dimethylaminonaphthalene-1-sulfonyl chloride dansyl chloride. Ticagrelor contains a secondary amino group which reacts with dansyl chloride in the presence of 0.5 M sodium bicarbonate pH 10 to yield a highly fluorescent derivative that is measured at 445 nm after excitation at 340 nm. Different experimental parameters affecting the fluorescence intensities were carefully studied and optimized. The proposed method was also validated according to ICH guidelines. The fluorescence concentration plot was rectilinear over the range of 20.0 200.0 ng mL1, with a coefficient of determination of 0.9999 with a limit of detection LOD of 0.14 ng mL1 and the limit of quantitation LOQ of 0.46 ng mL1. The proposed method was applied successfully to the analysis of TCG in spiked human plasma and pharmaceutical dosage forms with good accuracy and precision.

Makale detayları

Spectrofluorimetric analysis of ticagrelor in pharmaceutical formulations and spiked human plasma using 1-dimethylaminonaphthalene-5-sulphonyl chloride reagent

Yazarlar:

Cem Onal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.765-773

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348000

pISSN:

2630-6344

Ticagrelor

antiplatelet

spectrofluorimetry

dansyl chloride

derivatization

validation

pharmaceutical preparations

plasma samples

Sayı

Makale detayları

Yazarlar: Cem Onal

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.24 Issue 5, pp.765-773

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001348000

pISSN: 2630-6344

Sayı

Yazarlar:

Cem Onal

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.24 Issue 5, pp.765-773

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001348000

pISSN:

2630-6344