Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

Ebubekir Dirican; Yasemin Kaya

Makale detayları

Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

Yazarlar:

Ebubekir Dirican | Yasemin Kaya

Yayıncı:

Cukurova University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Cukurova Medical Journal Vol.48 Issue 1, pp.216-226

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282251

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: Type 2 diabetes mellitus T2DM is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1 -94 ATTG insdel polymorphisms on the expression of apoptosis genes and telomere length TL in patients with T2DM compared with healthy individuals. Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized. Results: There was a significant difference between the NFKB1 -94 insdel genotype patients and the control group OR:0.4792 0.2345-1.011. However, the distribution of other genotypealleles insins and deldel showed no difference between T2DM and control groups. The allelic frequency of NFKB1 -94 insdel was 0.4550.235 for the T2DM group and 0.4350.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals. Conclusion: NFKB1 -94 insdel polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.

 Purpose: Type 2 diabetes mellitus T2DM is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1 -94 ATTG insdel polymorphisms on the expression of apoptosis genes and telomere length TL in patients with T2DM compared with healthy individuals. Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized. Results: There was a significant difference between the NFKB1 -94 insdel genotype patients and the control group OR:0.4792 0.2345-1.011. However, the distribution of other genotypealleles insins and deldel showed no difference between T2DM and control groups. The allelic frequency of NFKB1 -94 insdel was 0.4550.235 for the T2DM group and 0.4350.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals. Conclusion: NFKB1 -94 insdel polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.

 Purpose: Type 2 diabetes mellitus T2DM is a heterogeneous, chronic, and metabolic disease that affects a significant proportion of the global population. This study aimed to evaluate the effect of NFKB1 -94 ATTG insdel polymorphisms on the expression of apoptosis genes and telomere length TL in patients with T2DM compared with healthy individuals. Materials and Methods: Sixty-nine T2DM patients and sixty healthy people were enrolled in the study. DNA and RNA were isolated from the blood samples. NFKB1 genotypes were identified by Sanger sequencing. For TL analyses and to investigate the expression of the caspase-3, caspase-9, bax, and bcl2 genes, RT-PCR was utilized. Results: There was a significant difference between the NFKB1 -94 insdel genotype patients and the control group OR:0.4792 0.2345-1.011. However, the distribution of other genotypealleles insins and deldel showed no difference between T2DM and control groups. The allelic frequency of NFKB1 -94 insdel was 0.4550.235 for the T2DM group and 0.4350.165 for the control group. An increase in the mRNA expression of caspase-3, caspase-9 and Bax genes was observed in the T2DM group compared with the healthy group, while a decrease in the Bcl2 gene was found in the T2DM group. TL in T2DM patients was shorter than in healthy individuals. Conclusion: NFKB1 -94 insdel polymorphisms show significant differences in T2DM patients. We observed that apoptosis was activated and TL was shortened in patients with T2DM. However, no relationship between NFKB1 polymorphisms and apoptosis and TL could not be determined.

 Amaç: Tip 2 diabetes mellitus T2DM, dünya nüfusunun büyük bir bölümünü etkileyen kronik, metabolik ve heterojen bir hastalıktır. Bu çalışma, T2DMli hastalarda NFKB1 -94 ATTG insdel polimorfizmlerinin, apoptoz genlerinin ekspresyonu ve telomer uzunluğunu TU sağlıklı bireylerle karşılaştırmalı olarak değerlendirmeyi amaçladı. Gereç ve Yöntem: Çalışmaya 69 T2DM hastası ve 60 sağlıklı kişi dahil edildi. Kan örneklerinden DNA ve RNA izole edildi. NFKB1 genotipleri, Sanger sekansı ile tanımlandı. TU analizleri için ve kaspaz-3, kaspaz-9, bax ve bcl2 genlerinin ekspresyonunu araştırmak için RT-PCR kullanıldı. Bulgular: NFKB1 -94 insdel genotipli hastalar ile kontrol grubu arasında anlamlı bir fark vardı OR:0,4792 0,2345-1,011. Bununla birlikte, diğer genotipalellerin insins ve deldel dağılımı, T2DM ve kontrol grupları arasında fark göstermedi. NFKB1-94 insdelin alelik frekansı T2DM grubu için 0,4550,235 ve kontrol grubu için 0,4350,165idi. T2DM grubunda kaspaz-3, kaspaz-9 ve Bax genlerinin mRNA ekspresyonunda sağlıklı gruba göre artış gözlenirken, T2DM grubunda Bcl2 geninde azalma saptandı. T2DM hastalarında TU, sağlıklı bireylerden daha kısaydı. Sonuç: NFKB1 -94 insdel polimorfizmleri, T2DM hastalarında anlamlı farklılık göstermektedir. T2DMli hastalarda apoptozun aktive olduğunu ve TUnun kısaldığını gözlemledik. Ancak NFKB1 polimorfizmleri ile apoptoz ve TU arasında ilişki saptanamadı.

Makale detayları

Investigation of the relationship between NFKB1 polymorphisms and telomere length and apoptosis in patients with type-2 diabetes

Yazarlar:

Ebubekir Dirican | Yasemin Kaya

Yayıncı:

Cukurova University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Cukurova Medical Journal Vol.48 Issue 1, pp.216-226

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282251

pISSN:

2602-3032

T2DM

NFKB1

apoptosis

telomere length

Sanger sequencing

T2DM

NFKB1

apoptoz

telomere uzunluğu

Sanger sekans

Sayı

Makale detayları

Yazarlar: Ebubekir Dirican | Yasemin Kaya

Yayıncı: Cukurova University

Ana Başlıklar: Klinik Tıp Bilimleri

Dergi: Cukurova Medical Journal Vol.48 Issue 1, pp.216-226

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001282251

pISSN: 2602-3032

Sayı

Yazarlar:

Ebubekir Dirican | Yasemin Kaya

Yayıncı:

Cukurova University

Ana Başlıklar:

Klinik Tıp Bilimleri

Dergi:

Cukurova Medical Journal Vol.48 Issue 1, pp.216-226

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001282251

pISSN:

2602-3032