Colchicine-Induced Tetraploidy and Changes in Allele Frequencies in Colchicine-Treated Populations of Diploids Assessed with RAPD Markers in Gossypium arboreum L.

Saeed RAUF; Iftikhar Ahmad KHAN; Farooq Ahmad KHAN

Makale detayları

Colchicine-Induced Tetraploidy and Changes in Allele Frequencies in Colchicine-Treated Populations of Diploids Assessed with RAPD Markers in Gossypium arboreum L.

Yazarlar:

Saeed RAUF | Iftikhar Ahmad KHAN | Farooq Ahmad KHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.93-100

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813396

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Two cultivars of cotton G. arboreum L. cv. FDH-228 and HK-113 were treated with colchicine solution (1%) with 3 methods of application. Colchicine application at the seedling stage on shoot apex was successful. Tetraploid plants of arboreum (4x = 2n = 52) had larger vegetative, reproductive parts, but less frequent stomata and higher optical density of DNA than their normal diploid counterparts (2n = 26). Results also showed that individuals that escaped the duplicating effects of colchicine although treated, presented significant point mutagenic effects. Randomly Amplified Polymorphic DNA markers were used to evaluate diversity and similarities in populations treated with colchicine. Colchicine treatment generated differences beyond the levels of inter and intra-population variability. Contrary to expectations, colchicine-treated plants obtained by dipping in colchicine solution for 30 min did not contain more diversity than untreated populations.

 

Two cultivars of cotton G. arboreum L. cv. FDH-228 and HK-113 were treated with colchicine solution (1%) with 3 methods of application. Colchicine application at the seedling stage on shoot apex was successful. Tetraploid plants of arboreum (4x = 2n = 52) had larger vegetative, reproductive parts, but less frequent stomata and higher optical density of DNA than their normal diploid counterparts (2n = 26). Results also showed that individuals that escaped the duplicating effects of colchicine although treated, presented significant point mutagenic effects. Randomly Amplified Polymorphic DNA markers were used to evaluate diversity and similarities in populations treated with colchicine. Colchicine treatment generated differences beyond the levels of inter and intra-population variability. Contrary to expectations, colchicine-treated plants obtained by dipping in colchicine solution for 30 min did not contain more diversity than untreated populations.

Makale detayları

Colchicine-Induced Tetraploidy and Changes in Allele Frequencies in Colchicine-Treated Populations of Diploids Assessed with RAPD Markers in Gossypium arboreum L.

Yazarlar:

Saeed RAUF | Iftikhar Ahmad KHAN | Farooq Ahmad KHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.93-100

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813396

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Gossypium arboreum L.

Colchicine

induced ploidy

Cultivars

Mutagenic

RAPD markers

Variability

Sayı

Makale detayları

Yazarlar: Saeed RAUF | Iftikhar Ahmad KHAN | Farooq Ahmad KHAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.93-100

Belge Tipi: Makale

Yayın Tarihi: 1/2006

Belge Dili: Türkçe

Document ID (Org No.): 2000813396

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Saeed RAUF | Iftikhar Ahmad KHAN | Farooq Ahmad KHAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2006 Vol.30 Issue 2, pp.93-100

Belge Tipi:

Makale

Yayın Tarihi:

1/2006

Belge Dili:

Türkçe

Document ID (Org No.):

2000813396

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152