Optimization of Particle Size Distribution with Gaussian Analysis of Albumin Microcarriers Cross-linked by Natural Phenolic Compounds

Sultan Duman; Oğuz Akpolat; Fatma Ayhan

Makale detayları

Optimization of Particle Size Distribution with Gaussian Analysis of Albumin Microcarriers Cross-linked by Natural Phenolic Compounds

Yazarlar:

Sultan Duman | Oğuz Akpolat | Fatma Ayhan

Yayıncı:

Gazi University

Ana Başlıklar:

Engineering

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1886-1900

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331640

pISSN:

2147-1762

Anahtar kelimeler:

Özet:

 The biodegradation of albumin into natural products and nontoxicity besides its antigenicity has many advantages in controlled drug delivery of therapeutic agents. A bifunctional covalent bonding agent, glutaraldehyde is extensively used for linking amine groups of albümin microparticlesmicrocarriers AlbMCs. But its cytotoxicity and the rapid calcification of the glutaraldehyde-treated tissue limit the use of glutaraldehyde. Phenolic compound showed non-covalent and covalent chemical interactions with proteins. The objective of this research is to prepare three different natural phenolic compound cross-linkedstabilized AlbMCs and estimate the cross-linker concentration which is giving narrow size distributions since it is important to gain higher surface area. The influence of qallic acid GA, tannic acid TA and quercetin concentrations on AlbMCs size was investigated by Gaussian function analysis of microcarriers determined after optical micrograph measurements. Gallic acid GA stabilized AlbMCs have 3.35  0.71 μm average mean size distribution while it was 3.56  0.71 μm for Quercetin and 3.71  0.69 μm for TA stabilized microcarrier formations. Average mean particle size distribution of AlbMCs synthesized with synthetic cross-linker, glutaraldehyde was calculated as 5.12  0.50 μm. All statistical analysis were evaluated by MATLAB program. New approach for albumin microcarrier synthesis by using phenolic compounds as a cross-linker can be proposed as an alternative microcarrier preparation system with narrow size distributions.

 The biodegradation of albumin into natural products and nontoxicity besides its antigenicity has many advantages in controlled drug delivery of therapeutic agents. A bifunctional covalent bonding agent, glutaraldehyde is extensively used for linking amine groups of albümin microparticlesmicrocarriers AlbMCs. But its cytotoxicity and the rapid calcification of the glutaraldehyde-treated tissue limit the use of glutaraldehyde. Phenolic compound showed non-covalent and covalent chemical interactions with proteins. The objective of this research is to prepare three different natural phenolic compound cross-linkedstabilized AlbMCs and estimate the cross-linker concentration which is giving narrow size distributions since it is important to gain higher surface area. The influence of qallic acid GA, tannic acid TA and quercetin concentrations on AlbMCs size was investigated by Gaussian function analysis of microcarriers determined after optical micrograph measurements. Gallic acid GA stabilized AlbMCs have 3.35  0.71 μm average mean size distribution while it was 3.56  0.71 μm for Quercetin and 3.71  0.69 μm for TA stabilized microcarrier formations. Average mean particle size distribution of AlbMCs synthesized with synthetic cross-linker, glutaraldehyde was calculated as 5.12  0.50 μm. All statistical analysis were evaluated by MATLAB program. New approach for albumin microcarrier synthesis by using phenolic compounds as a cross-linker can be proposed as an alternative microcarrier preparation system with narrow size distributions.

 The biodegradation of albumin into natural products and nontoxicity besides its antigenicity has many advantages in controlled drug delivery of therapeutic agents. A bifunctional covalent bonding agent, glutaraldehyde is extensively used for linking amine groups of albümin microparticlesmicrocarriers AlbMCs. But its cytotoxicity and the rapid calcification of the glutaraldehyde-treated tissue limit the use of glutaraldehyde. Phenolic compound showed non-covalent and covalent chemical interactions with proteins. The objective of this research is to prepare three different natural phenolic compound cross-linkedstabilized AlbMCs and estimate the cross-linker concentration which is giving narrow size distributions since it is important to gain higher surface area. The influence of qallic acid GA, tannic acid TA and quercetin concentrations on AlbMCs size was investigated by Gaussian function analysis of microcarriers determined after optical micrograph measurements. Gallic acid GA stabilized AlbMCs have 3.35  0.71 μm average mean size distribution while it was 3.56  0.71 μm for Quercetin and 3.71  0.69 μm for TA stabilized microcarrier formations. Average mean particle size distribution of AlbMCs synthesized with synthetic cross-linker, glutaraldehyde was calculated as 5.12  0.50 μm. All statistical analysis were evaluated by MATLAB program. New approach for albumin microcarrier synthesis by using phenolic compounds as a cross-linker can be proposed as an alternative microcarrier preparation system with narrow size distributions.

 The biodegradation of albumin into natural products and nontoxicity besides its antigenicity has many advantages in controlled drug delivery of therapeutic agents. A bifunctional covalent bonding agent, glutaraldehyde is extensively used for linking amine groups of albümin microparticlesmicrocarriers AlbMCs. But its cytotoxicity and the rapid calcification of the glutaraldehyde-treated tissue limit the use of glutaraldehyde. Phenolic compound showed non-covalent and covalent chemical interactions with proteins. The objective of this research is to prepare three different natural phenolic compound cross-linkedstabilized AlbMCs and estimate the cross-linker concentration which is giving narrow size distributions since it is important to gain higher surface area. The influence of qallic acid GA, tannic acid TA and quercetin concentrations on AlbMCs size was investigated by Gaussian function analysis of microcarriers determined after optical micrograph measurements. Gallic acid GA stabilized AlbMCs have 3.35  0.71 μm average mean size distribution while it was 3.56  0.71 μm for Quercetin and 3.71  0.69 μm for TA stabilized microcarrier formations. Average mean particle size distribution of AlbMCs synthesized with synthetic cross-linker, glutaraldehyde was calculated as 5.12  0.50 μm. All statistical analysis were evaluated by MATLAB program. New approach for albumin microcarrier synthesis by using phenolic compounds as a cross-linker can be proposed as an alternative microcarrier preparation system with narrow size distributions.

Makale detayları

Optimization of Particle Size Distribution with Gaussian Analysis of Albumin Microcarriers Cross-linked by Natural Phenolic Compounds

Yazarlar:

Sultan Duman | Oğuz Akpolat | Fatma Ayhan

Yayıncı:

Gazi University

Ana Başlıklar:

Engineering

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1886-1900

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331640

pISSN:

2147-1762

Albumin microcarrier

Natural cross-linker

Gallic acid

Tannic acid

Quercetin

Sayı

Makale detayları

Optimization of Particle Size Distribution with Gaussian Analysis of Albumin Microcarriers Cross-linked by Natural Phenolic Compounds

Yazarlar: Sultan Duman | Oğuz Akpolat | Fatma Ayhan

Yayıncı: Gazi University

Ana Başlıklar: Engineering

Dergi: Gazi University Journal of Science Vol.37 Issue 4, pp.1886-1900

Belge Tipi: Makale

Yayın Tarihi: 1/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001331640

pISSN: 2147-1762

Albumin microcarrier

Natural cross-linker

Gallic acid

Tannic acid

Quercetin

Sayı

Yazarlar:

Sultan Duman | Oğuz Akpolat | Fatma Ayhan

Yayıncı:

Gazi University

Ana Başlıklar:

Engineering

Dergi:

Gazi University Journal of Science Vol.37 Issue 4, pp.1886-1900

Belge Tipi:

Makale

Yayın Tarihi:

1/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001331640

pISSN:

2147-1762