Molecular Biological Effects of Gundelia tournefortii (Kenger Herb) Extract on Saccharomyces cerevisiae Cell Growth: Oxidative Stress and Protective Role

Batuhan Yılmaz; Hacı Fırat; Seda Beyaz; Nasrullah Celayir; Abdullah Aslan

Makale detayları

Molecular Biological Effects of Gundelia tournefortii (Kenger Herb) Extract on Saccharomyces cerevisiae Cell Growth: Oxidative Stress and Protective Role

Yazarlar:

Batuhan Yılmaz | Hacı Fırat | Seda Beyaz | Nasrullah Celayir | Abdullah Aslan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Hücresel Etkileşimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359142

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Gundelia tournefortii Kenger plant is traditionally used for liver diseases, diabetes, chest pain, heart failure, stomach pain, vitiligo, diarrhea, and bronchitis. Additionally, it is known for its purgative, sedative, anti-inflammatory, antiparasitic, antiseptic, and antiemetic properties. This project investigates whether Gundelia tournefortii has a protective role against hydrogen peroxide H2O2-induced oxidative damage in Saccharomyces cerevisiae. In this study, four groups were established: Control group; H2O2 group 15 millimolar; G. tournefortii group 10; G. tournefortii 10  H2O2 15 millimolar group. S. cerevisiae cultures were grown at 30C for 1, 3, 5, and 24-hour intervals. Cell growth periods, lipid peroxidation malondialdehyde MDA analysis, glutathione GSH levels, and catalase activity CAT were measured using a spectrophotometer. The total protein concentration changes of S. cerevisiae cultures were determined by SDS-PAGE electrophoresis and calculated using the Bradford method. As a result, in the H2O2 group, MDA levels increased, while GSH levels and CAT activity decreased, indicating increased oxidative stress. In the G. tournefortii group, MDA levels decreased, while GSH levels and CAT activity increased, suggesting that this plant has a protective effect. Compared to the H2O2 group, the G. tournefortii  H2O2 group showed increased cell growth and protein concentration, indicating that this group provided stronger cellular defense against oxidative damage, significantly improving cellular functions. Keywords: Gundelia tournefortii Kenger plant, Hydrogen peroxide, Oxidative damage, Protein synthesis, Saccharomyces cerevisiae.

 Gundelia tournefortii Kenger plant is traditionally used for liver diseases, diabetes, chest pain, heart failure, stomach pain, vitiligo, diarrhea, and bronchitis. Additionally, it is known for its purgative, sedative, anti-inflammatory, antiparasitic, antiseptic, and antiemetic properties. This project investigates whether Gundelia tournefortii has a protective role against hydrogen peroxide H2O2-induced oxidative damage in Saccharomyces cerevisiae. In this study, four groups were established: Control group; H2O2 group 15 millimolar; G. tournefortii group 10; G. tournefortii 10  H2O2 15 millimolar group. S. cerevisiae cultures were grown at 30C for 1, 3, 5, and 24-hour intervals. Cell growth periods, lipid peroxidation malondialdehyde MDA analysis, glutathione GSH levels, and catalase activity CAT were measured using a spectrophotometer. The total protein concentration changes of S. cerevisiae cultures were determined by SDS-PAGE electrophoresis and calculated using the Bradford method. As a result, in the H2O2 group, MDA levels increased, while GSH levels and CAT activity decreased, indicating increased oxidative stress. In the G. tournefortii group, MDA levels decreased, while GSH levels and CAT activity increased, suggesting that this plant has a protective effect. Compared to the H2O2 group, the G. tournefortii  H2O2 group showed increased cell growth and protein concentration, indicating that this group provided stronger cellular defense against oxidative damage, significantly improving cellular functions. Keywords: Gundelia tournefortii Kenger plant, Hydrogen peroxide, Oxidative damage, Protein synthesis, Saccharomyces cerevisiae.

 Gundelia tournefortii Kenger plant is traditionally used for liver diseases, diabetes, chest pain, heart failure, stomach pain, vitiligo, diarrhea, and bronchitis. Additionally, it is known for its purgative, sedative, anti-inflammatory, antiparasitic, antiseptic, and antiemetic properties. This project investigates whether Gundelia tournefortii has a protective role against hydrogen peroxide H2O2-induced oxidative damage in Saccharomyces cerevisiae. In this study, four groups were established: Control group; H2O2 group 15 millimolar; G. tournefortii group 10; G. tournefortii 10  H2O2 15 millimolar group. S. cerevisiae cultures were grown at 30C for 1, 3, 5, and 24-hour intervals. Cell growth periods, lipid peroxidation malondialdehyde MDA analysis, glutathione GSH levels, and catalase activity CAT were measured using a spectrophotometer. The total protein concentration changes of S. cerevisiae cultures were determined by SDS-PAGE electrophoresis and calculated using the Bradford method. As a result, in the H2O2 group, MDA levels increased, while GSH levels and CAT activity decreased, indicating increased oxidative stress. In the G. tournefortii group, MDA levels decreased, while GSH levels and CAT activity increased, suggesting that this plant has a protective effect. Compared to the H2O2 group, the G. tournefortii  H2O2 group showed increased cell growth and protein concentration, indicating that this group provided stronger cellular defense against oxidative damage, significantly improving cellular functions. Keywords: Gundelia tournefortii Kenger plant, Hydrogen peroxide, Oxidative damage, Protein synthesis, Saccharomyces cerevisiae.

 Gundelia tournefortii Kenger plant is traditionally used for liver diseases, diabetes, chest pain, heart failure, stomach pain, vitiligo, diarrhea, and bronchitis. Additionally, it is known for its purgative, sedative, anti-inflammatory, antiparasitic, antiseptic, and antiemetic properties. This project investigates whether Gundelia tournefortii has a protective role against hydrogen peroxide H2O2-induced oxidative damage in Saccharomyces cerevisiae. In this study, four groups were established: Control group; H2O2 group 15 millimolar; G. tournefortii group 10; G. tournefortii 10  H2O2 15 millimolar group. S. cerevisiae cultures were grown at 30C for 1, 3, 5, and 24-hour intervals. Cell growth periods, lipid peroxidation malondialdehyde MDA analysis, glutathione GSH levels, and catalase activity CAT were measured using a spectrophotometer. The total protein concentration changes of S. cerevisiae cultures were determined by SDS-PAGE electrophoresis and calculated using the Bradford method. As a result, in the H2O2 group, MDA levels increased, while GSH levels and CAT activity decreased, indicating increased oxidative stress. In the G. tournefortii group, MDA levels decreased, while GSH levels and CAT activity increased, suggesting that this plant has a protective effect. Compared to the H2O2 group, the G. tournefortii  H2O2 group showed increased cell growth and protein concentration, indicating that this group provided stronger cellular defense against oxidative damage, significantly improving cellular functions.

Makale detayları

Molecular Biological Effects of Gundelia tournefortii (Kenger Herb) Extract on Saccharomyces cerevisiae Cell Growth: Oxidative Stress and Protective Role

Yazarlar:

Batuhan Yılmaz | Hacı Fırat | Seda Beyaz | Nasrullah Celayir | Abdullah Aslan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Hücresel Etkileşimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359142

pISSN:

2149-6366

Gundelia tournefortii (Kenger plant)

Hydrogen peroxide

Oxidative damage

Protein synthesis

Saccharomyces cerevisiae.Gundelia tournefortii (Kenger plant)

Hydrogen peroxide

Oxidative damage

Protein synthesis

Saccharomyces cerevisiae.

Sayı

Makale detayları

Yazarlar: Batuhan Yılmaz | Hacı Fırat | Seda Beyaz | Nasrullah Celayir | Abdullah Aslan

Yayıncı: Bingol University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü | Hücresel Etkileşimler

Dergi: Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.1-9

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001359142

pISSN: 2149-6366

Sayı

Yazarlar:

Batuhan Yılmaz | Hacı Fırat | Seda Beyaz | Nasrullah Celayir | Abdullah Aslan

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü | Hücresel Etkileşimler

Dergi:

Türk Doğa ve Fen Dergisi Vol.14 Issue 3, pp.1-9

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001359142

pISSN:

2149-6366