HISTOPATHOLOGICAL EFFECTS OF THIOUREA DIOXIDE ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio

Cansu AKBULUT; Büşra FİDAN; Mustafa ZENGİN; Nazan Deniz YÖN

Makale detayları

HISTOPATHOLOGICAL EFFECTS OF THIOUREA DIOXIDE ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio

Yazarlar:

Cansu AKBULUT | Büşra FİDAN | Mustafa ZENGİN | Nazan Deniz YÖN

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-255

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835441

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711

Özet:

 


Investigation
the histopathological effects of thiourea dioxide on intestine tissue of
zebrafish were aimed.

Thioureadioxide
is an organosulfur compound which is a strong reducing agent. It is generally
used as decolorisation agent, bleach in textile industry. In the other
industrial fields such as leather processing, photographic, paper, pulp and
board industry it has a wide range of use. Industrial waste products have been
contaminated aquatic environment day after day. 

After one-week
adaptation period zebrafish divided into three group (n=10) as one control and
2 experimental groups (3mM, 4.5mM). For investigating the effects of thiourea
dioxide, intestine tissues were dissected after 5 day of the exposure. Tissues
were fixed with 10% neutral buffered formalin and dehydration were carried out
in an ascending series of ethanol. Tissues were cleared in xylene, embedded in
paraffin wax and cut into 5 µm sections on a microtome. The sections were
stained with hematoxylin (H&E). Results were evaluated with light
microscope.








In control group
normal intestine histology have been observed. In 3 mM thiourea dioxide exposed
group, hypertrophy and edema at muscularis externa, degeneration and necrosis
at villi structure, deterioration and vacuolization at lamina propria and
increase in the number of goblet cells were observed. In 4.5 mM thiourea
dioxide exposed group, severe edema and hemorrhage in muscularis externa,
degeneration at villi structure and vacuolization at mucosa layer were
monitored.

.

Makale detayları

HISTOPATHOLOGICAL EFFECTS OF THIOUREA DIOXIDE ON INTESTINE TISSUE OF ZEBRAFISH (Danio rerio

Yazarlar: Cansu AKBULUT | Büşra FİDAN | Mustafa ZENGİN | Nazan Deniz YÖN

Yayıncı: Engin TUTKUN

Dergi: The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-255

Belge Tipi: Makale

Yayın Tarihi: 2/2017

Belge Dili: Türkçe

Document ID (Org No.): 2000835441

Sıklık: Üç Aylık

eISSN: 2149-4711

pISSN: 2149-4711

Sayı

Yazarlar:

Cansu AKBULUT | Büşra FİDAN | Mustafa ZENGİN | Nazan Deniz YÖN

Yayıncı:

Engin TUTKUN

Dergi:

The Turkish Journal Of Occupational / Environmental Medicine and Safety 2017 Vol.Volume 2 Issue İssue 1 (1), pp.1-255

Belge Tipi:

Makale

Yayın Tarihi:

2/2017

Belge Dili:

Türkçe

Document ID (Org No.):

2000835441

Sıklık:

Üç Aylık

eISSN:

2149-4711

pISSN:

2149-4711