Investigation of total phenolic and flavonoid content of Salvia willeana (Holmboe) Hedge, an endemic plant of Cyprus, and screening of its antioxidant and cholinesterase inhibitory properties

Tugba Ercetin; Hayrettin Ozan Gülcan

Makale detayları

Investigation of total phenolic and flavonoid content of Salvia willeana (Holmboe) Hedge, an endemic plant of Cyprus, and screening of its antioxidant and cholinesterase inhibitory properties

Yazarlar:

Tugba Ercetin | Hayrettin Ozan Gülcan

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.263-271

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346334

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Salvia willeana Holmboe Hedge is an endemic plant of Cyprus island. There have been limited number of studies on the plant, beside some analytical studies on its chemical content. This study aimed to investigate total phenolic and flavonoid content of the plant concomitant to some pharmacological activity screening studies including antioxidant and cholinesterase inhibition. Following obtaining different organic solvent and aqueous extracts of the dried aerial parts of the plant, 2-diphenyl-1-picrylhydrazyl DPPH radical scavenging activity, ferric-reducing antioxidant power FRAP, iron-chelation capacity assays, and modified Ellmans method were employed for screening antioxidant and cholinesterase inhibition studies. S. willeana extracts displayed low to moderate activities in cholinesterase inhibition assays. The ethyl acetate extracts of the leaves and the methanol extracts of the stem part of the plant displayed the highest acetylcholinesterase inhibitory potential i.e., 28.95  0.035, and 24.01  0.007, respectively, while the highest butyrylcholinesterase inhibitory potential was obtained in aqueous extracts of the stem at 0.5 mgmL i.e., 29.10  0.005. The methanol extracts of the leaves and flower of the plant provided the highest antioxidant activity in DPPH radical scavenging activity 67.94  0.003, and 45.07  0.001, respectively at 0.5 mgmL concentration. Ferrous iron chelating activity of the extracts were found weak in general. The plant was found rich in terms of its flavonoid and phenolic contents as shown in the ethyl acetate and methanol extracts of the leaves i.e., 547.55  3.12 mgg, and 300.24  1.23 mgg extract, respectively.

 Salvia willeana Holmboe Hedge is an endemic plant of Cyprus island. There have been limited number of studies on the plant, beside some analytical studies on its chemical content. This study aimed to investigate total phenolic and flavonoid content of the plant concomitant to some pharmacological activity screening studies including antioxidant and cholinesterase inhibition. Following obtaining different organic solvent and aqueous extracts of the dried aerial parts of the plant, 2-diphenyl-1-picrylhydrazyl DPPH radical scavenging activity, ferric-reducing antioxidant power FRAP, iron-chelation capacity assays, and modified Ellmans method were employed for screening antioxidant and cholinesterase inhibition studies. S. willeana extracts displayed low to moderate activities in cholinesterase inhibition assays. The ethyl acetate extracts of the leaves and the methanol extracts of the stem part of the plant displayed the highest acetylcholinesterase inhibitory potential i.e., 28.95  0.035, and 24.01  0.007, respectively, while the highest butyrylcholinesterase inhibitory potential was obtained in aqueous extracts of the stem at 0.5 mgmL i.e., 29.10  0.005. The methanol extracts of the leaves and flower of the plant provided the highest antioxidant activity in DPPH radical scavenging activity 67.94  0.003, and 45.07  0.001, respectively at 0.5 mgmL concentration. Ferrous iron chelating activity of the extracts were found weak in general. The plant was found rich in terms of its flavonoid and phenolic contents as shown in the ethyl acetate and methanol extracts of the leaves i.e., 547.55  3.12 mgg, and 300.24  1.23 mgg extract, respectively.

 Salvia willeana Holmboe Hedge is an endemic plant of Cyprus island. There have been limited number of studies on the plant, beside some analytical studies on its chemical content. This study aimed to investigate total phenolic and flavonoid content of the plant concomitant to some pharmacological activity screening studies including antioxidant and cholinesterase inhibition. Following obtaining different organic solvent and aqueous extracts of the dried aerial parts of the plant, 2-diphenyl-1-picrylhydrazyl DPPH radical scavenging activity, ferric-reducing antioxidant power FRAP, iron-chelation capacity assays, and modified Ellmans method were employed for screening antioxidant and cholinesterase inhibition studies. S. willeana extracts displayed low to moderate activities in cholinesterase inhibition assays. The ethyl acetate extracts of the leaves and the methanol extracts of the stem part of the plant displayed the highest acetylcholinesterase inhibitory potential i.e., 28.95  0.035, and 24.01  0.007, respectively, while the highest butyrylcholinesterase inhibitory potential was obtained in aqueous extracts of the stem at 0.5 mgmL i.e., 29.10  0.005. The methanol extracts of the leaves and flower of the plant provided the highest antioxidant activity in DPPH radical scavenging activity 67.94  0.003, and 45.07  0.001, respectively at 0.5 mgmL concentration. Ferrous iron chelating activity of the extracts were found weak in general. The plant was found rich in terms of its flavonoid and phenolic contents as shown in the ethyl acetate and methanol extracts of the leaves i.e., 547.55  3.12 mgg, and 300.24  1.23 mgg extract, respectively.

 Salvia willeana Holmboe Hedge is an endemic plant of Cyprus island. There have been limited number of studies on the plant, beside some analytical studies on its chemical content. This study aimed to investigate total phenolic and flavonoid content of the plant concomitant to some pharmacological activity screening studies including antioxidant and cholinesterase inhibition. Following obtaining different organic solvent and aqueous extracts of the dried aerial parts of the plant, 2-diphenyl-1-picrylhydrazyl DPPH radical scavenging activity, ferric-reducing antioxidant power FRAP, iron-chelation capacity assays, and modified Ellmans method were employed for screening antioxidant and cholinesterase inhibition studies. S. willeana extracts displayed low to moderate activities in cholinesterase inhibition assays. The ethyl acetate extracts of the leaves and the methanol extracts of the stem part of the plant displayed the highest acetylcholinesterase inhibitory potential i.e., 28.95  0.035, and 24.01  0.007, respectively, while the highest butyrylcholinesterase inhibitory potential was obtained in aqueous extracts of the stem at 0.5 mgmL i.e., 29.10  0.005. The methanol extracts of the leaves and flower of the plant provided the highest antioxidant activity in DPPH radical scavenging activity 67.94  0.003, and 45.07  0.001, respectively at 0.5 mgmL concentration. Ferrous iron chelating activity of the extracts were found weak in general. The plant was found rich in terms of its flavonoid and phenolic contents as shown in the ethyl acetate and methanol extracts of the leaves i.e., 547.55  3.12 mgg, and 300.24  1.23 mgg extract, respectively.

Makale detayları

Investigation of total phenolic and flavonoid content of Salvia willeana (Holmboe) Hedge, an endemic plant of Cyprus, and screening of its antioxidant and cholinesterase inhibitory properties

Yazarlar:

Tugba Ercetin | Hayrettin Ozan Gülcan

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.263-271

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346334

pISSN:

2630-6344

Salvia willeana

antioxidant activity

cholinesterase inhibitor

Alzheimer’s Disease

Sayı

Makale detayları

Investigation of total phenolic and flavonoid content of Salvia willeana (Holmboe) Hedge, an endemic plant of Cyprus, and screening of its antioxidant and cholinesterase inhibitory properties

Yazarlar: Tugba Ercetin | Hayrettin Ozan Gülcan

Yayıncı: Marmara University

Ana Başlıklar: Pharmacognosy | Pharmaceutical Chemistry

Dergi: Journal of Research in Pharmacy Vol.26 Issue 2, pp.263-271

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346334

pISSN: 2630-6344

Salvia willeana

antioxidant activity

cholinesterase inhibitor

Alzheimer’s Disease

Sayı

Yazarlar:

Tugba Ercetin | Hayrettin Ozan Gülcan

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacognosy | Pharmaceutical Chemistry

Dergi:

Journal of Research in Pharmacy Vol.26 Issue 2, pp.263-271

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346334

pISSN:

2630-6344