Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Mustafa Altındiş; Zafer Çetinkaya; Raike Kalaycı; İhsan H. Çiftçi; Alpaslan Arslan; Orhan C. Aktepe

doi:https://doi.org/10.5799/ahinjs.02.2011.01.0002

Makale detayları

Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Yazarlar:

Mustafa Altındiş | Zafer Çetinkaya | Raike Kalaycı | İhsan H. Çiftçi | Alpaslan Arslan | Orhan C. Aktepe

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2011 Vol.1 Issue 01, pp.5-9

Belge Tipi:

Makale

Yayın Tarihi:

2/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000488122

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/ahinjs.02.2011.01.0002

Özet:

 

Objectives: The aim of the present study was to evaluate the efficacy (recovery rate, time to detection and Drug Susceptibility Tests –DST- of Mycobacteria-only B460) of new colorimetric medium, Dio-TK and to compare it with routinely used conventional media, Lowenstein Jensen (LJ) and Bactec 460 TB culture system.    	Materials and methods: Totally 901 clinic specimens were investigated for assignment of tuberculosis by Ehrlich-Ziehl-Nielsen smear strain method, Lowenstein-Jensen, BACTEC 460TB and Dio-TK medium culture systems.    	Results: Nineteen of 901 clinic specimens (2.1%) were positive by any of these methods. 17 (89.5%) of these specimens positive found by smear strain method, 17 (89.5%) by Lowenstein-Jensen, 19 (100%) by BACTEC 460TB and 14 (73.7%) by Dio-TK medium. NAP and Niacin identification tests were applied to Mycobacterium strains. 12 (63.1%) of 19 isolates were identified as M.tuberculosis complex and 7 (36.9%) were identified as Mycobacterium other than tuberculosis (MOTT) bacilli. 10 (83.3%) of 12 M.tuberculosis complex strains were not resistant to any major drug. But one of 2 isolate was resistant to streptomycin and the other one isolate was resistant to both streptomycin and isoniazid.    	Conclusion: Our data suggest that some advantages (such as an early detection and differentiation mycobacterium growth from contamination) of the Dio-TK CS over other mycobacterial culture systems make it a practical and rapid system for daily use, and a suitable alternative to other currently available solid media, such as LJ, for detection time of mycobacteria and DST.

Makale detayları

Detection of Mycobacterium isolates with different methods and their resistance ratios against anti-tuberculosis drugs

Yazarlar: Mustafa Altındiş | Zafer Çetinkaya | Raike Kalaycı | İhsan H. Çiftçi | Alpaslan Arslan | Orhan C. Aktepe

Yayıncı: Sağlık Araştırmaları Derneği

Dergi: Journal of Microbiology and Infectious Diseases 2011 Vol.1 Issue 01, pp.5-9

Belge Tipi: Makale

Yayın Tarihi: 2/2011

Belge Dili: İngilizce

Document ID (Org No.): 2000488122

Sıklık: Üç Aylık

eISSN: 2146-9369

pISSN: 2146-3158

DOI: https://doi.org/10.5799/ahinjs.02.2011.01.0002

Sayı

Yazarlar:

Mustafa Altındiş | Zafer Çetinkaya | Raike Kalaycı | İhsan H. Çiftçi | Alpaslan Arslan | Orhan C. Aktepe

Yayıncı:

Sağlık Araştırmaları Derneği

Dergi:

Journal of Microbiology and Infectious Diseases 2011 Vol.1 Issue 01, pp.5-9

Belge Tipi:

Makale

Yayın Tarihi:

2/2011

Belge Dili:

İngilizce

Document ID (Org No.):

2000488122

Sıklık:

Üç Aylık

eISSN:

2146-9369

pISSN:

2146-3158

DOI:

https://doi.org/10.5799/ahinjs.02.2011.01.0002