Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?

Neslihan Cevik; Gülper Nacarkahya; Serkan Gürgül; Cem Horozoğlu

Makale detayları

Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?

Yazarlar:

Neslihan Cevik | Gülper Nacarkahya | Serkan Gürgül | Cem Horozoğlu

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Experimed Vol.14 Issue 3, pp.210-214

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001340140

pISSN:

2667-5846

Anahtar kelimeler:

Özet:

 Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 GLP-1 expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue. Materials and Methods: Adult Wistar albino rats n32 were randomly divided into four groups for 16 weeks of intervention. These groups were control C n8, obese Ob n8, sitagliptin CStg n8, and obese ObStg given sitagliptin n8. Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR. Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C p0.008. Additionally, a 4.03-fold increase in expression level was found in ObStg compared to Ob p0.02 and a 12.52-fold increase in expression level was found in ObStg compared to C p0.01. Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process.

 Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 GLP-1 expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue. Materials and Methods: Adult Wistar albino rats n32 were randomly divided into four groups for 16 weeks of intervention. These groups were control C n8, obese Ob n8, sitagliptin CStg n8, and obese ObStg given sitagliptin n8. Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR. Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C p0.008. Additionally, a 4.03-fold increase in expression level was found in ObStg compared to Ob p0.02 and a 12.52-fold increase in expression level was found in ObStg compared to C p0.01. Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process.

 Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 GLP-1 expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue. Materials and Methods: Adult Wistar albino rats n32 were randomly divided into four groups for 16 weeks of intervention. These groups were control C n8, obese Ob n8, sitagliptin CStg n8, and obese ObStg given sitagliptin n8. Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR. Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C p0.008. Additionally, a 4.03-fold increase in expression level was found in ObStg compared to Ob p0.02 and a 12.52-fold increase in expression level was found in ObStg compared to C p0.01. Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process.

 Objective: The aim of this study was to evaluate the contribution of sitagliptin, which is used in the treatment of type 2 diabetes mellitus due to its insulinotropic effects, to the levels of glucagon-like peptide-1 GLP-1 expressed in many systemic tissues in obesity, in liver, skeletal muscle, and fat tissue. Materials and Methods: Adult Wistar albino rats n32 were randomly divided into four groups for 16 weeks of intervention. These groups were control C n8, obese Ob n8, sitagliptin CStg n8, and obese ObStg given sitagliptin n8. Glp1r expression in rat liver, muscle, and adipose tissue was confirmed by quantitative real-time PCR. Results: No significant change was detected in Glp1r expression levels in muscle and fat tissue in 4 groups. A 10.64-fold increase in Glp1r gene expression was observed in Ob compared to C p0.008. Additionally, a 4.03-fold increase in expression level was found in ObStg compared to Ob p0.02 and a 12.52-fold increase in expression level was found in ObStg compared to C p0.01. Conclusion: The increased Glp1r expression intensity in obese individuals using sitagliptin compared with controls and obese individuals not using sitagliptin may play a role in the reorganization of liver metabolism that is impaired due to obesity, such as the gluconeogenesis process.

Makale detayları

Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?

Yazarlar:

Neslihan Cevik | Gülper Nacarkahya | Serkan Gürgül | Cem Horozoğlu

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Experimed Vol.14 Issue 3, pp.210-214

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001340140

pISSN:

2667-5846

Sitagliptin

GLP-1

obesity

liver metabolism

Glp1r

GLP1R

Sayı

Makale detayları

Could Increased Glp1r Expression via Sitagliptin in the GLP-1/GLP-1 Receptor Axis in the DietInduced Obesity Rat Model be Important in Liver Metabolism?

Yazarlar: Neslihan Cevik | Gülper Nacarkahya | Serkan Gürgül | Cem Horozoğlu

Yayıncı: Istanbul University

Ana Başlıklar: Biochemistry and Cell Biology (Other)

Dergi: Experimed Vol.14 Issue 3, pp.210-214

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001340140

pISSN: 2667-5846

Sitagliptin

GLP-1

obesity

liver metabolism

Glp1r

GLP1R

Sayı

Yazarlar:

Neslihan Cevik | Gülper Nacarkahya | Serkan Gürgül | Cem Horozoğlu

Yayıncı:

Istanbul University

Ana Başlıklar:

Biochemistry and Cell Biology (Other)

Dergi:

Experimed Vol.14 Issue 3, pp.210-214

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001340140

pISSN:

2667-5846