Comparison of the effects of recombinant human epidermal growth factor (rhEGF) and alendronate sodium on tibial fracture healing in rats: an experimental study

Hüseyin Emre Tepedelenlioğlu; Ferid Abdullaliyev; Asim Ahmadov; Saadet Özen Akarca Dizakar; Candan Özoğul; Erdinç Esen

Makale detayları

Comparison of the effects of recombinant human epidermal growth factor (rhEGF) and alendronate sodium on tibial fracture healing in rats: an experimental study

Yazarlar:

Hüseyin Emre Tepedelenlioğlu | Ferid Abdullaliyev | Asim Ahmadov | Saadet Özen Akarca Dizakar | Candan Özoğul | Erdinç Esen

Yayıncı:

Cukurova University

Ana Başlıklar:

Ortopedi | Histoloji ve Embriyoloji

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.703-711

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327863

pISSN:

2602-3032

Anahtar kelimeler:

Özet:

 Purpose: The objective of this study is to compare the effects of recombinant human epidermal growth factor rhEGF on bone healing with those of alendronate, a bisphosphonate widely used in practice. Materials and Methods: An iatrogenic fracture was created in the tibial shaft of 24 Sprague-Dawley rats with osteotome and fixed with an intramedullary Kirschner wire K-wire. After surgery, Group 1 was given 0.2 mgkgday of oral alendronate sodium on postoperative Day 1 to 28, Group 2 received a single dose of 0.5 mgkg of intraosseous rhEGF on postoperative Days 1 and 14, and Group 3 was followed for a total of four weeks with oral saline. At the end of Week 4, the animals were euthanized and the lower extremities were removed by stripping the soft tissues without damaging the callus. Tissue samples of groups were prepared and and stained with hematoxylin-eosin. After staining, histological scoring was performed to evaluate the degree of union. Results: Alendronate sodium group demonstrated a mean histological score of 6.95 1.28. The rhEGF group had a lower mean score of 4.851.66. The placebo group exhibited the least progress in bone healing with a mean score of 4.101.68. The histological score was significantly higher in the alendronate sodium group compared to both the rhEGF and placebo groups. There was also a statistically significant difference between the rhEGF and placebo groups in terms of scores. Conclusion: Alendronate sodium enhanced fracture healing processes in rats. The role of rhEGF in bone healing requires further exploration. As the understanding of bisphosphonates and growth factors in bone healing evolves, the strategies for optimizing patient care in orthopedic settings are expected to be developed.

 Purpose: The objective of this study is to compare the effects of recombinant human epidermal growth factor rhEGF on bone healing with those of alendronate, a bisphosphonate widely used in practice. Materials and Methods: An iatrogenic fracture was created in the tibial shaft of 24 Sprague-Dawley rats with osteotome and fixed with an intramedullary Kirschner wire K-wire. After surgery, Group 1 was given 0.2 mgkgday of oral alendronate sodium on postoperative Day 1 to 28, Group 2 received a single dose of 0.5 mgkg of intraosseous rhEGF on postoperative Days 1 and 14, and Group 3 was followed for a total of four weeks with oral saline. At the end of Week 4, the animals were euthanized and the lower extremities were removed by stripping the soft tissues without damaging the callus. Tissue samples of groups were prepared and and stained with hematoxylin-eosin. After staining, histological scoring was performed to evaluate the degree of union. Results: Alendronate sodium group demonstrated a mean histological score of 6.95 1.28. The rhEGF group had a lower mean score of 4.851.66. The placebo group exhibited the least progress in bone healing with a mean score of 4.101.68. The histological score was significantly higher in the alendronate sodium group compared to both the rhEGF and placebo groups. There was also a statistically significant difference between the rhEGF and placebo groups in terms of scores. Conclusion: Alendronate sodium enhanced fracture healing processes in rats. The role of rhEGF in bone healing requires further exploration. As the understanding of bisphosphonates and growth factors in bone healing evolves, the strategies for optimizing patient care in orthopedic settings are expected to be developed.

 Purpose: The objective of this study is to compare the effects of recombinant human epidermal growth factor rhEGF on bone healing with those of alendronate, a bisphosphonate widely used in practice. Materials and Methods: An iatrogenic fracture was created in the tibial shaft of 24 Sprague-Dawley rats with osteotome and fixed with an intramedullary Kirschner wire K-wire. After surgery, Group 1 was given 0.2 mgkgday of oral alendronate sodium on postoperative Day 1 to 28, Group 2 received a single dose of 0.5 mgkg of intraosseous rhEGF on postoperative Days 1 and 14, and Group 3 was followed for a total of four weeks with oral saline. At the end of Week 4, the animals were euthanized and the lower extremities were removed by stripping the soft tissues without damaging the callus. Tissue samples of groups were prepared and and stained with hematoxylin-eosin. After staining, histological scoring was performed to evaluate the degree of union. Results: Alendronate sodium group demonstrated a mean histological score of 6.95 1.28. The rhEGF group had a lower mean score of 4.851.66. The placebo group exhibited the least progress in bone healing with a mean score of 4.101.68. The histological score was significantly higher in the alendronate sodium group compared to both the rhEGF and placebo groups. There was also a statistically significant difference between the rhEGF and placebo groups in terms of scores. Conclusion: Alendronate sodium enhanced fracture healing processes in rats. The role of rhEGF in bone healing requires further exploration. As the understanding of bisphosphonates and growth factors in bone healing evolves, the strategies for optimizing patient care in orthopedic settings are expected to be developed.

 Amaç: Bu çalışmanın amacı rekombinant insan epidermal büyüme faktörünün rhEGF kemik iyileşmesi üzerindeki etkilerini pratikte yaygın olarak kullanılan bir bifosfonat olan alendronat ile karşılaştırmaktır. Gereç ve Yöntem: Osteotom ile 24 Sprague-Dawley sıçanın tibial şaftında iyatrojenik kırık oluşturuldu ve intramedüller Kirschner teli K-teli ile tespit edildi. Operasyondan sonra, Grup 1e postoperatif 1 günden 28. güne kadar 0,2 mgkggün oral alendronat sodyum, Grup 2ye postoperatif 1 ve 14. günlerde tek doz 0,5 mgkg intraosseöz rhEGF verilmiş ve Grup 3 ise toplam dört hafta boyunca oral salin ile takip edilmiştir. Dördüncü haftanın sonunda hayvanlara ötenazi uygulandı ve alt ekstremiteler kallusa zarar vermeden yumuşak dokuları sıyrılarak çıkarıldı. Gruplara ait doku örnekleri hazırlandı ve hematoksilen-eozin ile boyandı. Boyama sonrasında kaynama derecesini değerlendirmek için histolojik skorlama yapıldı. Bulgular: Alendronat sodyum grubu ortalama 6.95 1.28 histolojik skor gösterdi. RhEGF grubunun ortalama skoru 4.851.66 ile daha düşüktü. Plasebo grubu 4.101.68 ortalama skor ile kemik iyileşmesinde en az ilerleme gösteren grup olmuştur. Alendronat sodyum grubunda histolojik skor hem rhEGF hem de plasebo gruplarına kıyasla anlamlı derecede yüksekti. Skorlar açısından rhEGF ve plasebo grupları arasında da istatistiksel olarak anlamlı bir fark vardı. Sonuç: Alendronat sodyum sıçanlarda kırık iyileşme süreçlerini geliştirmiştir. rhEGFnin kemik iyileşmesindeki rolü daha fazla araştırma gerektirmektedir. Kemik iyileşmesinde bifosfonatlar ve büyüme faktörlerinin anlaşılması geliştikçe, ortopedik ortamlarda hasta bakımını optimize etmek için stratejilerin geliştirilmesi beklenmektedir.

Makale detayları

Comparison of the effects of recombinant human epidermal growth factor (rhEGF) and alendronate sodium on tibial fracture healing in rats: an experimental study

Yazarlar:

Hüseyin Emre Tepedelenlioğlu | Ferid Abdullaliyev | Asim Ahmadov | Saadet Özen Akarca Dizakar | Candan Özoğul | Erdinç Esen

Yayıncı:

Cukurova University

Ana Başlıklar:

Ortopedi | Histoloji ve Embriyoloji

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.703-711

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327863

pISSN:

2602-3032

bisphosphanate

rhEGF

Fracture

Nonunion

Bone HealingBisfosfonat

rhEGF

Kırık İyileşmesi

Sayı

Makale detayları

Yazarlar: Hüseyin Emre Tepedelenlioğlu | Ferid Abdullaliyev | Asim Ahmadov | Saadet Özen Akarca Dizakar | Candan Özoğul | Erdinç Esen

Yayıncı: Cukurova University

Ana Başlıklar: Ortopedi | Histoloji ve Embriyoloji

Dergi: Cukurova Medical Journal Vol.49 Issue 3, pp.703-711

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001327863

pISSN: 2602-3032

Sayı

Yazarlar:

Hüseyin Emre Tepedelenlioğlu | Ferid Abdullaliyev | Asim Ahmadov | Saadet Özen Akarca Dizakar | Candan Özoğul | Erdinç Esen

Yayıncı:

Cukurova University

Ana Başlıklar:

Ortopedi | Histoloji ve Embriyoloji

Dergi:

Cukurova Medical Journal Vol.49 Issue 3, pp.703-711

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001327863

pISSN:

2602-3032