Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients

Duygu Yolal Ertural; Ümit Çınkır; Nurcan Aras

Makale detayları

Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients

Yazarlar:

Duygu Yolal Ertural | Ümit Çınkır | Nurcan Aras

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Genel Tıp Dergisi Vol.34 Issue 3, pp.332-336

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319222

pISSN:

2602-3741

Anahtar kelimeler:

Özet:

 BackgroundAims: Type 2 diabetes T2D whose prevalance differs in different populations is a multifactorial disease. T2D is describes a group of clinical syndromes resulting from glucose metabolism disorders triggered by genetic or environmental factors. Insulin-like growth factor 2 mRNA binding protein 2 IGF2BP2 gene participates in insulin signaling pathway and is involved in insulin secretion. SNPs in one of these genes, IGF2BP2 gene rs1470579 and rs4402960, have been reported to partially increase the risk of type II diabetes. The aim of this study was to investigate in a Turkish population identified associations of IGF2BP2 variants rs4402960 and rs1470579 with T2D. Methods: We genotyped two SNPs of IGF2BP2 gene, rs1470579 and rs4402960 in 100 healthy individuals and 100 patients. DNA isolation was performed on peripheral blood samples from patients and healthy groups. The molecular analysis of rs1470579 and rs4402960 polymorphisms of IGF2BP2 gene of each individual was performed by using Real-Time PCR Applied Biosystems method. Relationships of genotypes and alleles frequency of IGF2BP2 polymorphisms and T2D were examined by Chi-square or Likelihood ratio tests. Results: As a result of the genotype and allele distributions; there was association between type II diabetes patients and control group for IGF2BP2 rs1470579 AC gene polymorphism p0.0123. The frequency of AC genotype in patients is more than the control group. However, there was no statistically significant difference genotype distribution between the type 2 diabetes patients and control group for IGF2BP2 rs4402960 GT gene polymorphisms. There was no association between the patients and the control group for TT and GGGT genotype distribution p0.8847. Conclusions: The results showed that the IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with T2D in a Turkish population OR  2.002, 95 CI 1.1703.426, p  0.05; OR  1.879, 95 CI 1.1103.182, p 0.05. This is the first study between IGF2BP2 gene polymorphisms and type II diabetes in Turkish population. Keywords: IGF2BP2, T2D, Polymorphism.

 BackgroundAims: Type 2 diabetes T2D whose prevalance differs in different populations is a multifactorial disease. T2D is describes a group of clinical syndromes resulting from glucose metabolism disorders triggered by genetic or environmental factors. Insulin-like growth factor 2 mRNA binding protein 2 IGF2BP2 gene participates in insulin signaling pathway and is involved in insulin secretion. SNPs in one of these genes, IGF2BP2 gene rs1470579 and rs4402960, have been reported to partially increase the risk of type II diabetes. The aim of this study was to investigate in a Turkish population identified associations of IGF2BP2 variants rs4402960 and rs1470579 with T2D. Methods: We genotyped two SNPs of IGF2BP2 gene, rs1470579 and rs4402960 in 100 healthy individuals and 100 patients. DNA isolation was performed on peripheral blood samples from patients and healthy groups. The molecular analysis of rs1470579 and rs4402960 polymorphisms of IGF2BP2 gene of each individual was performed by using Real-Time PCR Applied Biosystems method. Relationships of genotypes and alleles frequency of IGF2BP2 polymorphisms and T2D were examined by Chi-square or Likelihood ratio tests. Results: As a result of the genotype and allele distributions; there was association between type II diabetes patients and control group for IGF2BP2 rs1470579 AC gene polymorphism p0.0123. The frequency of AC genotype in patients is more than the control group. However, there was no statistically significant difference genotype distribution between the type 2 diabetes patients and control group for IGF2BP2 rs4402960 GT gene polymorphisms. There was no association between the patients and the control group for TT and GGGT genotype distribution p0.8847. Conclusions: The results showed that the IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with T2D in a Turkish population OR  2.002, 95 CI 1.1703.426, p  0.05; OR  1.879, 95 CI 1.1103.182, p 0.05. This is the first study between IGF2BP2 gene polymorphisms and type II diabetes in Turkish population. Keywords: IGF2BP2, T2D, Polymorphism.

 BackgroundAims: Type 2 diabetes T2D whose prevalance differs in different populations is a multifactorial disease. T2D is describes a group of clinical syndromes resulting from glucose metabolism disorders triggered by genetic or environmental factors. Insulin-like growth factor 2 mRNA binding protein 2 IGF2BP2 gene participates in insulin signaling pathway and is involved in insulin secretion. SNPs in one of these genes, IGF2BP2 gene rs1470579 and rs4402960, have been reported to partially increase the risk of type II diabetes. The aim of this study was to investigate in a Turkish population identified associations of IGF2BP2 variants rs4402960 and rs1470579 with T2D. Methods: We genotyped two SNPs of IGF2BP2 gene, rs1470579 and rs4402960 in 100 healthy individuals and 100 patients. DNA isolation was performed on peripheral blood samples from patients and healthy groups. The molecular analysis of rs1470579 and rs4402960 polymorphisms of IGF2BP2 gene of each individual was performed by using Real-Time PCR Applied Biosystems method. Relationships of genotypes and alleles frequency of IGF2BP2 polymorphisms and T2D were examined by Chi-square or Likelihood ratio tests. Results: As a result of the genotype and allele distributions; there was association between type II diabetes patients and control group for IGF2BP2 rs1470579 AC gene polymorphism p0.0123. The frequency of AC genotype in patients is more than the control group. However, there was no statistically significant difference genotype distribution between the type 2 diabetes patients and control group for IGF2BP2 rs4402960 GT gene polymorphisms. There was no association between the patients and the control group for TT and GGGT genotype distribution p0.8847. Conclusions: The results showed that the IGF2BP2 gene rs1470579 and rs4402960 polymorphisms were associated with T2D in a Turkish population OR  2.002, 95 CI 1.1703.426, p  0.05; OR  1.879, 95 CI 1.1103.182, p 0.05. This is the first study between IGF2BP2 gene polymorphisms and type II diabetes in Turkish population. Keywords: IGF2BP2, T2D, Polymorphism.

 Amaç: Prevalansı farklı toplumlarda farklılık gösteren Tip 2 Diyabet T2D multifaktöriyel bir hastalıktır. T2D, genetik veya çevresel faktörler tarafından tetiklenen glikoz metabolizması bozukluklarından kaynaklanan bir grup klinik sendromu tanımlamaktadır. İnsülin benzeri büyüme faktörü 2 mRNA bağlayıcı protein 2 IGF2BP2 geni, insülin sinyal yolağına katılır ve insülin salgılanmasında rol oynar. Bu gendeki polimorfizmlerin rs1470579 ve rsS4402960 diyabet riskini kısmen artırdığı bildirilmiştir. Bu çalışmanın amacı, Türk toplumunda rs4402960 ve rs1470579 IGF2BP2 varyantlarının T2D ile olan ilişkilerini araştırmaktır. Gereç ve Yöntem: IGF2BP2 geninin iki SNPsini, rs1470579 ve rs4402960, 100 sağlıklı bireyde ve 100 hastada genotiplendirdik. DNA izolasyonu hasta ve sağlıklı gruplardan alınan periferik kan örnekleri üzerinde gerçekleştirilmiştir. Her bireyin IGF2BP2 genine ait rs1470579 ve rs4402960 polimorfizmlerinin moleküler analizi Real-Time PCR Applied Biosystems yöntemi kullanılarak gerçekleştirilmiştir. IGF2BP2 polimorfizmlerinin genotip ve alel sıklığı ile T2D arasındaki ilişkiler Ki-kare veya Olabilirlik oranı testleri ile incelenmiştir. Bulgular: Genotip ve alel dağılımları sonucunda; IGF2BP2 rs1470579 AC gen polimorfizmi için tip II diyabet hastaları ile kontrol grubu arasında ilişki bulunmuştur p0.0123. Hastalarda AC genotipinin sıklığı kontrol grubundan daha fazladır. Ancak, IGF2BP2 rs4402960 GT gen polimorfizmleri için T2D hastaları ve kontrol grubu arasında genotip dağılımı açısından istatistiksel olarak anlamlı bir fark bulunmamıştır. TT ve GGGT genotip dağılımı için hastalar ve kontrol grubu arasında bir ilişki bulunmamıştır p0.8847. Sonuçlar: Sonuçlar, IGF2BP2 geni rs1470579 ve rs4402960 polimorfizmlerinin Türk toplumunda T2D ile ilişkili olduğunu göstermiştir OR  2.002, 95 CI 1.170-3.426, p  0.05; OR  1.879, 95 CI 1.110-3.182, p  0.05. Bu çalışma, Türk toplumunda IGF2BP2 gen polimorfizmleri ile T2D arasındaki ilk çalışmadır. Anahtar Kelimeler: IGF2BP2, T2D, Polimorfizm.

Makale detayları

Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients

Yazarlar:

Duygu Yolal Ertural | Ümit Çınkır | Nurcan Aras

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Genel Tıp Dergisi Vol.34 Issue 3, pp.332-336

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319222

pISSN:

2602-3741

IGF2BP2

T2D

PolymorphismIGF2BP2

Tip 2 Diyabet

Polimorfizm

Sayı

Makale detayları

Investigation of Insulin-Like Growth Factor 2 mRNA Binding Protein 2 Gene Polymorphisms in Type 2 Diabetes Patients

Yazarlar: Duygu Yolal Ertural | Ümit Çınkır | Nurcan Aras

Yayıncı: Selcuk University

Ana Başlıklar: Tıbbi Genetik (Kanser Genetiği hariç)

Dergi: Genel Tıp Dergisi Vol.34 Issue 3, pp.332-336

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001319222

pISSN: 2602-3741

IGF2BP2

T2D

PolymorphismIGF2BP2

Tip 2 Diyabet

Polimorfizm

Sayı

Yazarlar:

Duygu Yolal Ertural | Ümit Çınkır | Nurcan Aras

Yayıncı:

Selcuk University

Ana Başlıklar:

Tıbbi Genetik (Kanser Genetiği hariç)

Dergi:

Genel Tıp Dergisi Vol.34 Issue 3, pp.332-336

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001319222

pISSN:

2602-3741