Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

SEVİL KÖSE; FATİMA AERTS KAYA; EMİR BAKİ DENKBAŞ; PETEK KORKUSUZ; FAHRİYE DUYGU ÇETİNKAYA

Makale detayları

Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

Yazarlar:

SEVİL KÖSE | FATİMA AERTS KAYA | EMİR BAKİ DENKBAŞ | PETEK KORKUSUZ | FAHRİYE DUYGU ÇETİNKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.410-419

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811730

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Polyhydroxybutyrate (PHB) is a polymer used to restore tissues or regenerate bones. However, its compatibility with human bone marrow-derived mesenchymal stem cells (MSCs) has not been examined. PHB membranes of random (r-PHB) or aligned (a-PHB) electrospun nanofibers that generate bone and spinal axon scaffolds were combined with human MSCs. The adhesion and proliferation of cells on these membranes were examined. The orientation of cells on PHB membranes was analyzed by confocal microscopy and scanning electron microscopy (SEM). The MSCs maintained their characteristic properties on the membranes, adhered to the membranes, and preserved their viability. The cell morphology was different when they were grown on differentially designed matrices. Cells expanded on the a-PHB membrane and showed fibroid-like morphology. Conversely, cells interacting with the r-PHB membrane were located homogeneously and demonstrated polygonal morphology. The adhesion and proliferation of human MSCs was higher on the a-PHB membrane than on the randomly oriented one. Fiber orientation influenced the phenotype and biological behavior of human MSCs. This property may be useful in the selection of specifically designed scaffolds for the desired tasks. The results of this preliminary study indicated that PHB membranes designed for bone or nerve tissue engineering are compatible with human MSCs.

Makale detayları

Evaluation of biocompatibility of random or aligned electrospun polyhydroxybutyrate scaffolds combined with human mesenchymal stem cells

Yazarlar: SEVİL KÖSE | FATİMA AERTS KAYA | EMİR BAKİ DENKBAŞ | PETEK KORKUSUZ | FAHRİYE DUYGU ÇETİNKAYA

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.410-419

Belge Tipi: Makale

Yayın Tarihi: 3/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811730

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

SEVİL KÖSE | FATİMA AERTS KAYA | EMİR BAKİ DENKBAŞ | PETEK KORKUSUZ | FAHRİYE DUYGU ÇETİNKAYA

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.410-419

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811730

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152