Redox gene expression of adipose-derived stem cells in response to soft hydrogel

FAHSAI KANTAWONG; THASANEEYA KUBOKI; SATORU KIDOAKI

Makale detayları

Redox gene expression of adipose-derived stem cells in response to soft hydrogel

Yazarlar:

FAHSAI KANTAWONG | THASANEEYA KUBOKI | SATORU KIDOAKI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.682-691

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811839

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Abstract: Adipose-derived stem cells (ADSCs) showed morphological change to a neuron-like shape, and presented neuronal lineage bias when cultured on a very soft surface. To gain basic insight into gene expression relating to neuronal lineage bias in ADSCs, we examined the correlation between the gene expression levels of neuronal markers and redox proteins that were considered to have a close relation with lineage specification in stem cells. ADSCs were cultured on gelatinous soft hydrogel for 1-2 weeks. The time course changes in the expressions of neuronal genes (TUBB3 and NSE) and redox genes (TRX1, SOD1, SOD2, PRX2, GSTT1, and GSTP1) were monitored using real-time PCR. It was found that the TUBB3 gene had significantly upregulated compared to the control condition for tissue culture polystyrene, indicating that the neuronal gene expression of ADSCs could be achieved on soft hydrogel without the addition of any supplement. The expressions of the TRX1 and SOD1 genes were also observed to have significantly upregulated on the soft hydrogels. The results demonstrate that the upregulation of the neural marker of TUBB3 in the ADSCs correlates well with the upregulation of the redox genes of TRX1 and SOD1, when cultured on appropriate soft hydrogel substrates.

 

Abstract: Adipose-derived stem cells (ADSCs) showed morphological change to a neuron-like shape, and presented neuronal lineage bias when cultured on a very soft surface. To gain basic insight into gene expression relating to neuronal lineage bias in ADSCs, we examined the correlation between the gene expression levels of neuronal markers and redox proteins that were considered to have a close relation with lineage specification in stem cells. ADSCs were cultured on gelatinous soft hydrogel for 1-2 weeks. The time course changes in the expressions of neuronal genes (TUBB3 and NSE) and redox genes (TRX1, SOD1, SOD2, PRX2, GSTT1, and GSTP1) were monitored using real-time PCR. It was found that the TUBB3 gene had significantly upregulated compared to the control condition for tissue culture polystyrene, indicating that the neuronal gene expression of ADSCs could be achieved on soft hydrogel without the addition of any supplement. The expressions of the TRX1 and SOD1 genes were also observed to have significantly upregulated on the soft hydrogels. The results demonstrate that the upregulation of the neural marker of TUBB3 in the ADSCs correlates well with the upregulation of the redox genes of TRX1 and SOD1, when cultured on appropriate soft hydrogel substrates.

Makale detayları

Redox gene expression of adipose-derived stem cells in response to soft hydrogel

Yazarlar:

FAHSAI KANTAWONG | THASANEEYA KUBOKI | SATORU KIDOAKI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.682-691

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811839

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Soft hydrogel

neuronal gene

adipose-derived stem cells

oxidative stress

redox gene

Sayı

Makale detayları

Redox gene expression of adipose-derived stem cells in response to soft hydrogel

Yazarlar: FAHSAI KANTAWONG | THASANEEYA KUBOKI | SATORU KIDOAKI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.682-691

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811839

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Soft hydrogel

neuronal gene

adipose-derived stem cells

oxidative stress

redox gene

Sayı

Yazarlar:

FAHSAI KANTAWONG | THASANEEYA KUBOKI | SATORU KIDOAKI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.682-691

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811839

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152