Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes

ANA COSTE; DANA SUTEU; IOAN BACILA; CONSTANTIN DELIU; SERGIU VALIMAREANU; ADELA HALMAGYI

Makale detayları

Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes

Yazarlar:

ANA COSTE | DANA SUTEU | IOAN BACILA | CONSTANTIN DELIU | SERGIU VALIMAREANU | ADELA HALMAGYI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.638-648

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811896

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene ββ\beta-cyclase gene (LCY-B) from leaves. The highest shoot regeneration after cryopreservation was 70% (Pontica) following 24 h osmoprotection in 0.5 M sucrose and 30 min dehydration in plant vitrification solution 2 (PVS2). The data inferred from AFLP showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues compared with the noncryopreserved controls. Although a single nucleotide polymorphism, a G→T transversion, was identified in position 1139 in Capriciu and Darsirius, this was not due to the cryopreservation process itself, since it was detected in both cryopreserved and control samples. Thus, sequencing of LCY-B gene from leaves revealed no DNA damages after cryopreservation and subsequent in vitro regeneration. Our results indicate that cryostorage by droplet vitrification is an efficient and reliable technique to preserve the selected tomato genotypes and to regenerate true-to-type plants.

 

Shoot tips of five tomato (Lycopersicon esculentum Mill.) genotypes were successfully cryopreserved by droplet vitrification. Recovered plants were studied for genetic stability by two different assays: amplified fragment length polymorphism (AFLP) using 4 primer combinations and sequencing of lycopene ββ\beta-cyclase gene (LCY-B) from leaves. The highest shoot regeneration after cryopreservation was 70% (Pontica) following 24 h osmoprotection in 0.5 M sucrose and 30 min dehydration in plant vitrification solution 2 (PVS2). The data inferred from AFLP showed no genetic dissimilarities between in vitro regenerants derived from cryopreserved tissues compared with the noncryopreserved controls. Although a single nucleotide polymorphism, a G→T transversion, was identified in position 1139 in Capriciu and Darsirius, this was not due to the cryopreservation process itself, since it was detected in both cryopreserved and control samples. Thus, sequencing of LCY-B gene from leaves revealed no DNA damages after cryopreservation and subsequent in vitro regeneration. Our results indicate that cryostorage by droplet vitrification is an efficient and reliable technique to preserve the selected tomato genotypes and to regenerate true-to-type plants.

Makale detayları

Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes

Yazarlar:

ANA COSTE | DANA SUTEU | IOAN BACILA | CONSTANTIN DELIU | SERGIU VALIMAREANU | ADELA HALMAGYI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.638-648

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811896

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

AFLP

droplet-vitrification

genetic stability

LCY-B

tomato

Sayı

Makale detayları

Genetic integrity assessment of cryopreserved tomato (Lycopersicon esculentum Mill.) genotypes

Yazarlar: ANA COSTE | DANA SUTEU | IOAN BACILA | CONSTANTIN DELIU | SERGIU VALIMAREANU | ADELA HALMAGYI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.638-648

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811896

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

AFLP

droplet-vitrification

genetic stability

LCY-B

tomato

Sayı

Yazarlar:

ANA COSTE | DANA SUTEU | IOAN BACILA | CONSTANTIN DELIU | SERGIU VALIMAREANU | ADELA HALMAGYI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 4, pp.638-648

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811896

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152