EVALUATION OF AST FAST ES/NF AGAR MEDIUM FOR RAPID DETECTION OF IMIPENEM RESISTANCE IN CLINICAL KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII ISOLATES

Aybala Temel; Yamaç Tekintaş; Mine Hoşgör Limoncu; Feriha Cilli

Makale detayları

EVALUATION OF AST FAST ES/NF AGAR MEDIUM FOR RAPID DETECTION OF IMIPENEM RESISTANCE IN CLINICAL KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII ISOLATES

Yazarlar:

Aybala Temel | Yamaç Tekintaş | Mine Hoşgör Limoncu | Feriha Cilli

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 2, pp.430-437

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282827

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: Resistant gram-negative microorganisms, especially Klebsiella pneumoniae and Acinetobacter baumannii, cause life-threatening infections. Hence, it is critical to determine the antibiotic susceptibility of clinical isolates rapidly and accurately. Nowadays, carbapenem resistance is determined by phenotypicgenotypic methods. For phenotypic methods disk diffusion, microdilution, differences in specificity and sensitivity can often occur. Since molecular methods such as polymerase chain reaction giving relatively more reliable results, are labor-intensive and costly, new fast, reliable and economical methods are needed. Material and Method: The performance of chromogenic AST Fast ESNF agar for the EUCAST standard disk diffusion method was investigated. Four K. pneumoniae and five A. baumannii isolates, which were determined to be carbapenem resistant by molecular method and automated system results, were included in the study. Result and Discussion: The inhibition zone diameters were became measurable within 4-6 hours in AST Fast ESNF and the susceptibility categories were interpreted according to the manufacturers recommendations. The results of disk diffusion test performed with MHA, were largely consistent with the results obtained with the AST Fast ESNF. Considering that the incubation period recommended by EUCAST in the standard disk diffusion test method is 16-20 hours and the sensitivity category is measured at the end of this period, it is thought that the chromogenic medium may provide a significant advantage.

 Objective: Resistant gram-negative microorganisms, especially Klebsiella pneumoniae and Acinetobacter baumannii, cause life-threatening infections. Hence, it is critical to determine the antibiotic susceptibility of clinical isolates rapidly and accurately. Nowadays, carbapenem resistance is determined by phenotypicgenotypic methods. For phenotypic methods disk diffusion, microdilution, differences in specificity and sensitivity can often occur. Since molecular methods such as polymerase chain reaction giving relatively more reliable results, are labor-intensive and costly, new fast, reliable and economical methods are needed. Material and Method: The performance of chromogenic AST Fast ESNF agar for the EUCAST standard disk diffusion method was investigated. Four K. pneumoniae and five A. baumannii isolates, which were determined to be carbapenem resistant by molecular method and automated system results, were included in the study. Result and Discussion: The inhibition zone diameters were became measurable within 4-6 hours in AST Fast ESNF and the susceptibility categories were interpreted according to the manufacturers recommendations. The results of disk diffusion test performed with MHA, were largely consistent with the results obtained with the AST Fast ESNF. Considering that the incubation period recommended by EUCAST in the standard disk diffusion test method is 16-20 hours and the sensitivity category is measured at the end of this period, it is thought that the chromogenic medium may provide a significant advantage.

 Objective: Resistant gram-negative microorganisms, especially Klebsiella pneumoniae and Acinetobacter baumannii, cause life-threatening infections. Hence, it is critical to determine the antibiotic susceptibility of clinical isolates rapidly and accurately. Nowadays, carbapenem resistance is determined by phenotypicgenotypic methods. For phenotypic methods disk diffusion, microdilution, differences in specificity and sensitivity can often occur. Since molecular methods such as polymerase chain reaction giving relatively more reliable results, are labor-intensive and costly, new fast, reliable and economical methods are needed. Material and Method: The performance of chromogenic AST Fast ESNF agar for the EUCAST standard disk diffusion method was investigated. Four K. pneumoniae and five A. baumannii isolates, which were determined to be carbapenem resistant by molecular method and automated system results, were included in the study. Result and Discussion: The inhibition zone diameters were became measurable within 4-6 hours in AST Fast ESNF and the susceptibility categories were interpreted according to the manufacturers recommendations. The results of disk diffusion test performed with MHA, were largely consistent with the results obtained with the AST Fast ESNF. Considering that the incubation period recommended by EUCAST in the standard disk diffusion test method is 16-20 hours and the sensitivity category is measured at the end of this period, it is thought that the chromogenic medium may provide a significant advantage.

 Amaç: Klebsiella pneumoniae ve Acinetobacter baumannii başta olmak üzere dirençli gram negatif mikroorganizmalar yaşamı tehdit eden enfeksiyonlara neden olmaktadır. Bu nedenle klinik izolatların antibiyotik duyarlılıklarının en kısa sürede ve doğru tespiti kritik öneme sahiptir. Günümüzde karbapenem direnci fenotipik veveya genotipik yöntemlerle belirlenmektedir. Fenotipik yöntemlerde disk difüzyon, mikrodilüsyon hem özgüllük hem de duyarlılık açısından farklılıklar sıklıkla ortaya çıkabilmektedir. Nispeten daha güvenilir sonuçlar veren polimeraz zincir reaksiyonu gibi moleküler metotlar ise iş gücü gerektiren ve yüksek maliyetli yöntemler olduğundan, antibiyotik direncini saptamaya yönelik hızlı, güvenilir ve ekonomik yeni yöntemlere ihtiyaç duyulmaktadır. Gereç ve Yöntem: Çalışmada kromojenik AST Fast ESNF besiyerinin EUCAST standart disk difüzyon metoduyla antibiyotik duyarlılıklarının saptanmasında kullanımının uygunluğu araştırılmıştır. Karbapenem dirençli olduğu moleküler yöntemotomatize sistemle belirlenen dört K.pneumoniae ve beş A.baumannii izolatıyla çalışılmıştır. Sonuç ve Tartışma: Çalışma sonucunda AST Fast ESNF besiyerinde inhibisyon zon çaplarının 4-6 saat içerisinde ölçülebilir duruma geldiği belirlenmiş ve üretici firmanın önerilerine uygun şekilde izolatların duyarlılıkları belirlenmiştir. Mueller Hinton Agar kullanılarak gerçekleştirilen disk difüzyon test sonuçlarının, AST Fast ESNF agar kromojenik besiyeriyle elde edilen sonuçlar ile büyük oranda uyumlu olduğu belirlenmiştir. Standart disk difüzyon test yönteminde önerilen inkübasyon süresinin 16-20 saat olduğu ve ancak bu süre sonunda duyarlılık kategorisinin belirlendiği düşünüldüğünde kromojenik besiyerinin zaman açısından önemli bir avantaj sağlayabileceği düşünülmektedir.

Makale detayları

EVALUATION OF AST FAST ES/NF AGAR MEDIUM FOR RAPID DETECTION OF IMIPENEM RESISTANCE IN CLINICAL KLEBSIELLA PNEUMONIAE AND ACINETOBACTER BAUMANNII ISOLATES

Yazarlar:

Aybala Temel | Yamaç Tekintaş | Mine Hoşgör Limoncu | Feriha Cilli

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 2, pp.430-437

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282827

pISSN:

1015-3918

Acinetobacter baumanni

AST fast agar

chromogenic medium

disk diffusion method

Klebsiella pneumoniae

Acinetobacter baumanni

AST fast agar

disk difüzyon metodu

kromojenik besiyeri

Klebsiella pneumoniae

Sayı

Makale detayları

Yazarlar: Aybala Temel | Yamaç Tekintaş | Mine Hoşgör Limoncu | Feriha Cilli

Yayıncı: Ankara University

Ana Başlıklar: Eczacılık ve İlaç Bilimleri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 2, pp.430-437

Belge Tipi: Makale

Yayın Tarihi: 5/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001282827

pISSN: 1015-3918

Sayı

Yazarlar:

Aybala Temel | Yamaç Tekintaş | Mine Hoşgör Limoncu | Feriha Cilli

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 2, pp.430-437

Belge Tipi:

Makale

Yayın Tarihi:

5/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282827

pISSN:

1015-3918