Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

Namık Kemal Yücel

Makale detayları

Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

Yazarlar:

Namık Kemal Yücel

Yayıncı:

Selcuk University

Ana Başlıklar:

Sebze Yetiştirme ve Islahı

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 3, pp.571-578

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333560

pISSN:

2458-8377

Anahtar kelimeler:

Özet:

 In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C callus, R regeneration and V3 media recommended by Dumas de Valux et al. 1982 were used as nutrient media. However, as C callus medium, Dumas de Vaulx et al. 1982 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield 36.4 of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. 1982, from 1 mgl 2.4-D 1 mgl Kinetin  120 gl sucrose medium, the highest plant yield 33.8 of the Kemer variety was reported by Dumas de Vaulx et al. 1982, obtained from 5 mgl 2.4-D  5 mgl Kinetin  120 gl sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.

 In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C callus, R regeneration and V3 media recommended by Dumas de Valux et al. 1982 were used as nutrient media. However, as C callus medium, Dumas de Vaulx et al. 1982 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield 36.4 of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. 1982, from 1 mgl 2.4-D 1 mgl Kinetin  120 gl sucrose medium, the highest plant yield 33.8 of the Kemer variety was reported by Dumas de Vaulx et al. 1982, obtained from 5 mgl 2.4-D  5 mgl Kinetin  120 gl sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.

 In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C callus, R regeneration and V3 media recommended by Dumas de Valux et al. 1982 were used as nutrient media. However, as C callus medium, Dumas de Vaulx et al. 1982 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield 36.4 of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. 1982, from 1 mgl 2.4-D 1 mgl Kinetin  120 gl sucrose medium, the highest plant yield 33.8 of the Kemer variety was reported by Dumas de Vaulx et al. 1982, obtained from 5 mgl 2.4-D  5 mgl Kinetin  120 gl sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.

 In plant breeding studies, anther culture is used to breed hybrid varieties, to transform tetraplaid plants that can be obtained through interspecific somatic hybridization into fertile dihaploid plants, and in recent years, to obtain diploid in protoplast fusion of haploid embryogenic calli obtained from anther culture. The frequency of obtaining haploid plants in anther culture depends on many factors such as the variety, the season in which it was taken and the use of suitable media for regeneration. In this study, it was aimed to determine the appropriate regeneration environment in the anther culture of Aydın Siyahı and Kemer eggplant varieties, which are widely grown in our country. In the anther culture study, C callus, R regeneration and V3 media recommended by Dumas de Valux et al. 1982 were used as nutrient media. However, as C callus medium, Dumas de Vaulx et al. 1982 3 different doses of 2,4 D, Kinetin and sucrose of the C medium reported were tested. The highest plant yield 36.4 of the Aydin Siyahı variety was obtained from the modified Dumas de Vaulx et al. 1982, from 1 mgl 2.4-D 1 mgl Kinetin  120 gl sucrose medium, the highest plant yield 33.8 of the Kemer variety was reported by Dumas de Vaulx et al. 1982, obtained from 5 mgl 2.4-D  5 mgl Kinetin  120 gl sucrose medium. Of the 79 plants obtained from anther culture and whose ploidy level was examined, 60 were determined as haploid, 13 as diploid, 4 as triploid, 1 as tetraploid and 1 as mixoploid.

Makale detayları

Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

Yazarlar:

Namık Kemal Yücel

Yayıncı:

Selcuk University

Ana Başlıklar:

Sebze Yetiştirme ve Islahı

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 3, pp.571-578

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333560

pISSN:

2458-8377

Solanum melongena

anther culture

haphoid plant

Sayı

Makale detayları

Obtaining Haploid Plants Via Anther Culture in Some Eggplant Varieties

Yazarlar: Namık Kemal Yücel

Yayıncı: Selcuk University

Ana Başlıklar: Sebze Yetiştirme ve Islahı

Dergi: Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 3, pp.571-578

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001333560

pISSN: 2458-8377

Solanum melongena

anther culture

haphoid plant

Sayı

Yazarlar:

Namık Kemal Yücel

Yayıncı:

Selcuk University

Ana Başlıklar:

Sebze Yetiştirme ve Islahı

Dergi:

Selcuk Journal of Agriculture and Food Sciences Vol.38 Issue 3, pp.571-578

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333560

pISSN:

2458-8377