Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis

İfakat Tülay ÇAĞATAY; Jon G. H. HICKFORD

Makale detayları

Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis

Yazarlar:

İfakat Tülay ÇAĞATAY | Jon G. H. HICKFORD

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.53-59

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000768536

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain reaction (PCR). On the basis of PCR and DNA sequencing, 6 new D. nodosus isolates were identified and characterized from NZ. These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.

 

Dichelobacter nodosus is an essential causative agent of footrot in ruminants, particularly in sheep, goats and cattle. In this study, more than 100 footrot samples were collected from 4 different farming regions in New Zealand (NZ). Selective media were chosen and isolation and routine growth conditions were optimized for NZ D. nodosus serotypes. Approximately 1000 primary plates were anaerobically subcultured several times and examined with Gram staining in order to detect single colonies of D. nodosus. Both the variable region and a part of the conserved region of fimbrial subunit gene (fimA) were amplified from bacterial DNA using polymerase chain reaction (PCR). On the basis of PCR and DNA sequencing, 6 new D. nodosus isolates were identified and characterized from NZ. These sequences of D. nodosus fimA gene from NZ were genetically different from previously reported strains in the GenBank (NCBI) and commercial vaccine strains.

Makale detayları

Characterization of Footrot Bacteria Dichelobacter nodosus Using PCR Amplification and DNA Sequence Analysis

Yazarlar: İfakat Tülay ÇAĞATAY | Jon G. H. HICKFORD

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.53-59

Belge Tipi: Makale

Yayın Tarihi: 12/2005

Belge Dili: Türkçe

Document ID (Org No.): 2000768536

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Footrot

Dichelobacter nodosus

fimA gene

PCR

DNA sequencing

Sayı

Yazarlar:

İfakat Tülay ÇAĞATAY | Jon G. H. HICKFORD

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2006 Vol.30 Issue 1, pp.53-59

Belge Tipi:

Makale

Yayın Tarihi:

12/2005

Belge Dili:

Türkçe

Document ID (Org No.):

2000768536

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128