Limnophila aromatica (Lamk.) Merr.’nın Boğum ve Boğum Arası Eksplantlarından In Vitro Sürgün Rejenerasyonu

Muhammet DOĞAN

Makale detayları

Limnophila aromatica (Lamk.) Merr.’nın Boğum ve Boğum Arası Eksplantlarından In Vitro Sürgün Rejenerasyonu

Yazarlar:

Muhammet DOĞAN

Yayıncı:

Igdir University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Journal of the Institute of Science and Technology 2018 Vol.8 Issue 3, pp.77-84

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000465724

Sıklık:

Üç Aylık

eISSN:

2536-4618

Özet:

 


Limnophila aromatica (Lamk.) Merr. is an important medicinal and aromatic plant widely used in

the traditional medicine. In this study, nodal and internodal explants of L. aromatica (Lamk.) Merr. were cultured

for multiple and rapid production on Murashige and Skoog (MS) medium containing combinations of 0.10 mg

L-1 Gibberellic acid (GA3) and 0.05, 0.10, 0.20, 0.40, 0.80 and 1.60 mg L-1 Thidiazuron (TDZ) for eight weeks.

In general, high shoot regeneration percentages were obtained for both types of explants. In nodal explants, the

maximum number of shoots per explant (21.44) and the highest shoot length (1.68 cm) were determined in MS

medium containing 0.10 mg L-1 GA3 + 0.40 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.10 mg L-1 TDZ, respectively.

In internodal explants, the maximum number of shoots per explant (17.46) and the highest shoot length (1.60 cm)

were obtained in MS medium supplemented with 0.10 mg L-1 GA3 + 0.20 mg L-1 TDZ and 0.10 mg L-1 GA3 + 0.05

mg L-1 TDZ, respectively. With the effect of plant growth regulators, the nodal explants gave more regenerated

shoots than the internodal explants. Regenerated shoots were successfully rooted on MS medium containing 0.25

mg L-1 indole butyric acid (IBA). Successful adaptation of the rooted shoots to the aquarium environment has been

achieved.

.

Makale detayları

Limnophila aromatica (Lamk.) Merr.’nın Boğum ve Boğum Arası Eksplantlarından In Vitro Sürgün Rejenerasyonu

Yazarlar: Muhammet DOĞAN

Yayıncı: Igdir University

Ana Başlıklar: Yaşam bilimleri

Dergi: Journal of the Institute of Science and Technology 2018 Vol.8 Issue 3, pp.77-84

Belge Tipi: Makale

Yayın Tarihi: 9/2018

Belge Dili: Türkçe

Document ID (Org No.): 2000465724

Sıklık: Üç Aylık

eISSN: 2536-4618

Sayı

Yazarlar:

Muhammet DOĞAN

Yayıncı:

Igdir University

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Journal of the Institute of Science and Technology 2018 Vol.8 Issue 3, pp.77-84

Belge Tipi:

Makale

Yayın Tarihi:

9/2018

Belge Dili:

Türkçe

Document ID (Org No.):

2000465724

Sıklık:

Üç Aylık

eISSN:

2536-4618