Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey

İştar DOLAPÇI; Birsel ERDEM; Alper TEKELİ; Ebru US; Mehseti BAYRAMOVA; Begüm SARAN; Fikret ŞAHİN

Makale detayları

Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey

Yazarlar:

İştar DOLAPÇI | Birsel ERDEM | Alper TEKELİ | Ebru US | Mehseti BAYRAMOVA | Begüm SARAN | Fikret ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.447-458

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755095

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To investigate the characteristics of Salmonella serotype Typhi and Salmonella serotype Paratyphi B, which still remain significant in Turkey.   Materials and methods: Eighteen S. Typhi and 42 S. Paratyphi B strains isolated from clinical samples were investigated by plasmid profile analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and pulsed field gel electrophoresis (PFGE) methods.   Results: Plasmid profile analysis showed that 10 out of 18 (56%) S. Typhi isolates harbored 1 to 3 plasmids, and 11 out of 42 (26.2%) S. Paratyphi B isolates harbored 1 to 4 plasmids. PFGE performed with restriction enzyme AvrII grouped both S. Typhi and S. Paratyphi B isolates into 14 clusters; with restriction enzyme XbaI, S. Typhi and S. Paratyphi B isolates were grouped into 13 and 12 clusters, respectively.  Conclusion: These results showed that plasmid profile analysis and PFGE are reliable and discriminative methods complementing the phenotypic characters of S. Typhi and S. Paratyphi B isolates, and may contribute to the epidemiologic investigation of typhoid or paratyphoid diseases.  The study presented here is the first report on S. Typhi and S. Paratyphi B isolates from Turkey investigated by plasmid profile analysis, ERIC-PCR, and PFGE methods.

 

To investigate the characteristics of Salmonella serotype Typhi and Salmonella serotype Paratyphi B, which still remain significant in Turkey.   Materials and methods: Eighteen S. Typhi and 42 S. Paratyphi B strains isolated from clinical samples were investigated by plasmid profile analysis, enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR), and pulsed field gel electrophoresis (PFGE) methods.   Results: Plasmid profile analysis showed that 10 out of 18 (56%) S. Typhi isolates harbored 1 to 3 plasmids, and 11 out of 42 (26.2%) S. Paratyphi B isolates harbored 1 to 4 plasmids. PFGE performed with restriction enzyme AvrII grouped both S. Typhi and S. Paratyphi B isolates into 14 clusters; with restriction enzyme XbaI, S. Typhi and S. Paratyphi B isolates were grouped into 13 and 12 clusters, respectively.  Conclusion: These results showed that plasmid profile analysis and PFGE are reliable and discriminative methods complementing the phenotypic characters of S. Typhi and S. Paratyphi B isolates, and may contribute to the epidemiologic investigation of typhoid or paratyphoid diseases.  The study presented here is the first report on S. Typhi and S. Paratyphi B isolates from Turkey investigated by plasmid profile analysis, ERIC-PCR, and PFGE methods.

Makale detayları

Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey

Yazarlar:

İştar DOLAPÇI | Birsel ERDEM | Alper TEKELİ | Ebru US | Mehseti BAYRAMOVA | Begüm SARAN | Fikret ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.447-458

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755095

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Salmonella serotype Typhi

Salmonella serotype Paratyphi B

plasmid profiles

ERIC-PCR

PFGE

Turkey

Sayı

Makale detayları

Molecular analyses of Salmonella serotype Typhi and Salmonella serotype Paratyphi B strains isolated in Turkey

Yazarlar: İştar DOLAPÇI | Birsel ERDEM | Alper TEKELİ | Ebru US | Mehseti BAYRAMOVA | Begüm SARAN | Fikret ŞAHİN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.447-458

Belge Tipi: Makale

Yayın Tarihi: 2/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000755095

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Salmonella serotype Typhi

Salmonella serotype Paratyphi B

plasmid profiles

ERIC-PCR

PFGE

Turkey

Sayı

Yazarlar:

İştar DOLAPÇI | Birsel ERDEM | Alper TEKELİ | Ebru US | Mehseti BAYRAMOVA | Begüm SARAN | Fikret ŞAHİN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2010 Vol.40 Issue 3, pp.447-458

Belge Tipi:

Makale

Yayın Tarihi:

2/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000755095

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144